Summary
We have shown that if the medium supplemented with foetal calf serum (FCS) is continuously dialysed against a large volume of medium without FCS, the growth of human diploid fibroblasts on microcarriers is more rapid and yields are more consistent. This approach allows a considerable saving of serum.
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References
-Derynck et al. (1980). Nature285 542.
- Goeddel D.V. (1981). Communication at the 2nd Annual Intern. Congress for Interferon Research, San Francisco.
-Hitzeman, et al. (1981). Nature293 717.
-Lawrence J., Reitzer et al. (1979). The Journal of Biological Chemistry, vol. 254 N.8 pp. 2669–2676.
- Morandi M., Bandinelli L., and Valeri A. (1982). Experientia (in press).
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Morandi, M., Valeri, A. Continuous dialysis of cultures of human diploid cells (MRC 5) grown on microcarriers. Biotechnol Lett 4, 465–468 (1982). https://doi.org/10.1007/BF01134596
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DOI: https://doi.org/10.1007/BF01134596