Summary
Double mutants ofE. coli hyperproducing D-serine deaminase and β-galactosidase were isolated by two successive selection procedures in the chemostat. The specific activity of D-serine deaminase is 10 times higher and β-galactosidase 5 times higher compared with the fully induced original strain B 28.
Similar content being viewed by others
References
Bloom F. R. (1975)J. Bacteriol., 121, 1085–1091
Lederberg J. (1950)J. Bacteriol., 60, 381–392
McFall E. (1973)J. Bacteriol., 113, 781–785
Monod J., Cohen-Bazire D. and Cohn, M. (1951)Biochim. Biophys. Acta, 7, 585–599
Novick A. and Horiuchi T. (1961)Cold Spring Harbor Symp. Quant. Biol., 26, 239–245
Pardee A. B. and Prestidge L. S. (1955)J. Bacteriol., 70, 667–674
Pavlasová E., Stejskalová E., Novotný C. and Sikyta B. (1980a)Folia Microbiol 25, 16–23
Pavlasová E., Stejskalová E. and Sikyta B. (1980b)Biotechnology Letters, 2, 385–390
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pavlasová, E., Stejskalová, E. & Sikyta, B. Isolation ofEscherichia coli mutants with simultaneous hyperproduction of d-serine deaminase and β-galactosidase. Biotechnol Lett 3, 455–458 (1981). https://doi.org/10.1007/BF01134108
Issue Date:
DOI: https://doi.org/10.1007/BF01134108