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Molecular cloning and expression of s-(2-aminoethyl)-L-cysteine resistant aspartokinase gene ofCorynebacterium glutamicum

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Summary

A 2.9 kb DNA fragment encoding s-(2-aminoethyl)-l-cysteine (AEC) resistant aspartokinase ofCorynebacterium glutamicum was cloned on theC. glutamicum/E. coli shuttle vector pECCG117. A recombinant plasmid, designated pAK12, conferred the AEC resistance, the ability to excrete lysine and threonine, and the 3–5 fold increased specific activity of aspartokinase to host strain.

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Authors and Affiliations

  1. R&D Center, Cheil Foods & Chemicals Inc., 522 Majang, Dokpyong, Ichon, Korea

    Jong-Kwon Han, Jong-Won Oh, Hyune-Hwan Lee, Stephen Chung, Hyung-Hwan Hyun & Jae-Heung Lee

  2. Eugene Tech International, 1000 C, Lake street, 07446, Ramsey, NJ, USA

    Stephen Chung

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  1. Jong-Kwon Han
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  2. Jong-Won Oh
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  3. Hyune-Hwan Lee
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  4. Stephen Chung
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  5. Hyung-Hwan Hyun
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  6. Jae-Heung Lee
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Han, JK., Oh, JW., Lee, HH. et al. Molecular cloning and expression of s-(2-aminoethyl)-L-cysteine resistant aspartokinase gene ofCorynebacterium glutamicum . Biotechnol Lett 13, 721–726 (1991). https://doi.org/10.1007/BF01088176

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  • DOI: https://doi.org/10.1007/BF01088176

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