Skip to main content
SpringerLink
Log in

Effect of motilin on the lower esophageal sphincter of the opossum

  • Original Articles
  • Published:
The American Journal of Digestive Diseases Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

We have studied the effect of Yajima's synthetic motilin on the lower esophageal sphincter (LES) of the opossum and compared its potency to that of gastrin and its interaction with secretin. Dose-response curves for the LES were constructed from the intravenous bolus injection of graded doses of motilin and gastrin alone and motilin given against a background infusion of secretin. The smallest dose of motilin that elicited a significant response was 0.05 μg/kg, and the highest response was observed with the highest dose used in this study (1.0 μg/kg). Over a wide dose range, both motilin and gastrins were found to be equally potent in stimulating the LES of the opossum, and secretin caused inhibition of the LES pressure response to motilin. Therefore, we conclude that (1) motilin is a potent stimulant of the LES; (2) this response is dose dependent; (3) motilin's potency is similar to that of gastrin; and (4) secretin counteracts the effect of motilin on the LES.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Brown JC, Mutt V, Dryburgh JR: The further purification of motilin, a gastric motor activity stimulating polypeptide, from the mucosa of the small intestine of hogs. Can J Physiol Pharmacol 49:399–405, 1971

    Google Scholar 

  2. Brown JC, Parkes CO: Effect on fundic pouch motor activity of stimulatory and inhibitory fractions separated from pancreozymin. Gastroenterology 53:731–736, 1967

    Google Scholar 

  3. Brown JC: Presence of a gastric motor-stimulating property in duodenal extracts. Gastroenterology 52:225–229, 1967

    Google Scholar 

  4. Brown JC, Cook MA, Dryburgh JR: Motilin, a gastric motor activity-stimulating polypeptide: Final purification, amino acid composition and C-terminal residues. Gastroenterology 62:401–404, 1972

    Google Scholar 

  5. Brown JC, Cook MA, Dryburgh JR: Motilin, a gastric motor activity stimulating polypeptide: The complete amino acid sequence. Can J Biochem 51:533–537, 1973

    Google Scholar 

  6. Schubert H, Brown JC: Correction to the amino acid sequence of porcine motilin. Can J Biochem 52:7–8, 1974

    Google Scholar 

  7. Wunsch E, Brown JC, Deimer KH, Drees F, Musiol J, Scharf R, Stocker H, Thamm P, Wendlberger G: Zursynthese von norleucine-13-motilin. Z Naturforsch 28c:235–240, 1973

    Google Scholar 

  8. Yajima H, Kai Y, Kawatani H: Synthesis of the docosapeptide corresponding to the entire amino-acid sequence of porcine motilin. J C S Chem Commun 159–160, 1975

  9. Pearse AGE, Polak JM, Bloom SR, Adams C, Dryburgh JR, Brown JR: Enterochromaffin cells of the mammalian small intestine and the source of motilin. Virchows Arch (B) 16:111–120, 1974

    Google Scholar 

  10. Polak JM, Pearse AGE, Heath CM: Complete identification of endocrine cells in the gastrointestinal tract using semithin sections to identify motilin cells in human and animal intestine. Gut 16(3):225–229, 1975

    Google Scholar 

  11. jennewein HM, Hummelt H, Siewert R, Waldeck F: The motor stimulating effect of natural motilin on the lower esophageal sphincter, fundus, antrum and duodenum in dogs. Digestion 13:246–250, 1975

    Google Scholar 

  12. Lee KY, Hendricks J, Chey WY: Effects of gut hormones on motility of the antrum and duodenum in dogs. Gastroenterology 66:729, 1974 (abstract)

    Google Scholar 

  13. Ruppin H, Domschke S, Domschke W, Wunsch E, Jaeger E, Demling L: Effects of 13-Nle-motilin in man. Inhibition of gastric evacuation and stimulation of pepsin secretion. Scand J Gastroenterol 10:199–202, 1975

    Google Scholar 

  14. Castrasana M, Lee KY, Chey WY: Actions of synthetic porcine motilin octapeptide of cholecystokinin (CCK), gastric inhibitory polypeptide (GIP) and secretin on myoelectrical activity of gastric antrum and duodenum. Fed Proc 35:218, 1976 (abstract)

    Google Scholar 

  15. Christensen J, Lund GF: Esophageal responses to distension and electrical stimulation. J Clin Invest 48:408–419, 1969

    Google Scholar 

  16. Cohen S: The hormonal regulation of lower esophageal sphincter competence. Digestion 6:231–240, 1972

    Google Scholar 

  17. Castell DO: The lower esophageal sphincter—physiologic and clinical aspects. Ann Intern Med 83:390–401, 1975

    Google Scholar 

  18. Pope CE II: Pathophysiology and diagnosis of reflux esophagitis. Gastroenterology 70:445–454, 1976

    Google Scholar 

  19. Lipshutz W, Cohen S: Physiological determinants of lower esophageal sphincter function. Gastroenterology 61:16–24, 1971

    Google Scholar 

  20. Higgs RH, Smyth RD, Castell DO: Gastric alkalinization-effect of lower esophageal sphincter pressure and serum gastrin. N Engl J Med 291:486–490, 1974

    Google Scholar 

  21. McCallum RW, Kline MM, Sturdevant RAL: Studies on the mechanism of the lower esophageal sphincter pressure response to alkali ingestion in man. Gastroenterology 68:948, 1975 (abstract)

    Google Scholar 

  22. Brown JC, Dryburgh JR: Current status of motilin. Gastrointestinal Hormones Symposium. JC Thompson (ed), Austin, University of Texas Press, 1975, pp 549–554

    Google Scholar 

  23. Cohen S, Lipshutz W: Hormonal regulation of human lower esophageal sphincter competence: Interactions of gastrin and secretin. J Clin Invest 50:449–454, 1971

    Google Scholar 

  24. Morris DW, Schoen H, Brooks FP, Cohen S: Relationship of serum gastrin and lower esophageal sphincter pressure in normals and patients with antrectomy. Gastroenterology 66:750, 1974 (abstract)

    Google Scholar 

  25. Rosch W, Lux G, Domschke S, Domschke W, Wunsch E, Jaeger E, Demling L: Effect of 13-Nle-motilin on lower esophageal sphincter (LES) pressure in man. Gastroenterology 70:931, 1976 (abstract)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Isaac Gordon Center for Digestive Diseases and the Department of Medicine, Genesee Hospital, 224 Alexander Street, 14607, Rochester, New York

    Jorge G. Gutierrez MD, Krishan D. Thanik MD, William Y. Chey MD & Harauki Yajima PhD

  2. University of Rochester School of Medicine and Dentistry, Rochester, New York

    Jorge G. Gutierrez MD, Krishan D. Thanik MD, William Y. Chey MD & Harauki Yajima PhD

  3. Kyoto University, Kyoto, Japan

    Jorge G. Gutierrez MD, Krishan D. Thanik MD, William Y. Chey MD & Harauki Yajima PhD

Authors
  1. Jorge G. Gutierrez MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Krishan D. Thanik MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. William Y. Chey MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Harauki Yajima PhD
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gutierrez, J.G., Thanik, K.D., Chey, W.Y. et al. Effect of motilin on the lower esophageal sphincter of the opossum. Digest Dis Sci 22, 402–405 (1977). https://doi.org/10.1007/BF01071885

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01071885

Keywords

Search

Navigation