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Characteristics of chloride-dependent effects of acetylcholine and neutral aminoacids on neurons of the snail (Achatina)

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The response was investigated of neurons composing the cerebral ganglia inAchatina fulica (the Giant African snail) to application of acetylcholine (ACh), gamma-aminobutyric acid (GABA), and glycine (Gly). Chloride-dependent currents induced by these transmitters in 1 1/2-month old siblings were inhibited by dibutyryl-cAMP and strychnine. Inhibition of ACh response produced 10−8 M GABA was mimicked by application of dibutyryl-cAMP and isobutylmethylxanthine. Complete cross-desensitization was characteristic of both GABA- and Bly-induced response, but this effect did not occur when ACh and GABA (or Gly) were applied. A conclusion was reached on the basis of the pharmacological relationship between GABA- and Gly-induced response that these amino acids act on a single receptor — channel complex in the neurons of infant snails, whereas ACh-, GABA-, and Gly-induced chloride currents were not so related in cells of 4 year-oldAchatina.

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Literature cited

  1. L. A. Berezhnaya and P. M. Balaban, "Monosynaptic connections between identified mollusk neurons," Zh. Vyssh. Nervn. Deyat.,39, No. 2, 329–333 (1989).

    Google Scholar 

  2. S. A. Gapon, "Action of transmitter substances on identified neurons of the gastropod molluskPlanorbarius corneus," Zh. Evol. Biokhim. Fiziol.,19, No. 1, 38–45 (1983).

    Google Scholar 

  3. S. S. Dadalyan, K. V. Azatyan, and S. N. Airapetyan, "Action of low transmitter concentrations on counter-gradient transport of sodium ions and on intracellular level of cyclic nucleotides inHelix pomatia neurons," Neirokhimiya,7, No. 1, 18–25 (1988).

    Google Scholar 

  4. V. A. Dyatlov, "Action of serotonin on desensitization processes at receptors of gammaaminobutyric acid in mollusk neurons," Dokl. Akad. Nauk. SSSR,305, No. 2, 238–240 (1989).

    Google Scholar 

  5. V. A. Dyatlov, "Serotonin-induce modulation of glycine-induced ionic currents in mollusk neurons," Neirofiziologiyta,21, No. 3, 413–416 (1989).

    Google Scholar 

  6. B. V. Safronov, K. V. Baev, I. V. Batyeva, et al., "Effects of glycine and gamma aminobutyric acid on spinal cord neurons in the lamprey," Neirofiziologiya,20, No. 6, 823–825 (1988).

    Google Scholar 

  7. V. I. Skok, A. A. Selyanko, and V. A. Derkach, Neuronal cholinoreceptors [in Russian], Nauka, Moscow (1987).

    Google Scholar 

  8. T. M. Turpaev, O. P. Yurchenko, and K. Sh.-Rozha, "Two patterns of interaction between serotonin and acetylcholine in identified cerebral neurons ofHelix pomatia," Dokl. Akad. Nauk. SSSR,270, No. 6, 1505–1508 (1983).

    Google Scholar 

  9. A. R. Akopyan, N. K. Chemeris, V. I. Iljin, and B. N. Veprintev, "Serotonan, dopamine and intracellular cyclic AMP inhibit the responses of nicotinic cholinergic membrane in snail neurons," Brain Res.,201, No. 2, 480–484 (1980).

    Google Scholar 

  10. A. J. Bokisch and R. J. Walker, "The ionic mechanism associated with the action of Putative transmitters on identified neurons of the snail,Helix aspersa," Comp. Biochem. Physiol., Ser. C.,84, No. 2, 231–241 (1986).

    Google Scholar 

  11. S. Homma and C. M. Rovainen, "Conductance increases produced by glycine and gamma-aminobutyric acid in lamprey interneurons," J. Physiol.,279, No. 2, 231–252 (1978).

    Google Scholar 

  12. J. W. Johnson and P. Ascher, "Glycine potentiates the NMDA response in cultured mouse brain neurons," J. Physiol.,325, No. 6104, 529–531 (1987).

    Google Scholar 

  13. J. S. Kehoe, "Three acetylcholine receptors inAplysia neurones," J. Physiol.,225, No. 1, 115–146 (1972).

    Google Scholar 

  14. B. S. Ku, K. Isobe, and H. Takeuchi, "Pharmacological characteristics of four giant neurons identified in the cerebral ganglia of an African giant snail (Achatina fulica Ferussac)," Comp. Biochem. Physiol., Ser. C.,80, No. 1, 123–128 (1985).

    Google Scholar 

  15. Y. Oomura, T. Kita, and J. Maruhashi, "Chemical modification of several amino acid residues in the glycine receptors ofOnchidium neurons," Jpn. J. Physiol.,32, No. 5, 783–805 (1982).

    Google Scholar 

  16. H. Takeuchi, Y. Morimasa, M. Kohsaka, et al., "Concentrations des ions inorganiques dans l'hemolymphe de l'Escargot geant Africain (Achatina fulica Ferussac) selon l'etat de nutrition," C. R. S. Biol.,168, No. 4, 648–652 (1973).

    Google Scholar 

  17. P. J. Yarowsky and D. O. Carpenter, "A comparison of similar ionic responses to gammaaminobutyric acid acetylcholine," J. Neurophysiol.,41, No. 3, 531–541 (1978).

    Google Scholar 

  18. P. J. Yarowsky and D. O. Carpenter, "Receptors for gammoaminobutyric acid (GABA) onAplysia neurons," Brain Res.,144, No. 1, 75–94 (1978).

    Google Scholar 

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  1. V. A. Dyatlov
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A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 23, No. 1, pp. 35–43, January–February, 1991.

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Dyatlov, V.A. Characteristics of chloride-dependent effects of acetylcholine and neutral aminoacids on neurons of the snail (Achatina). Neurophysiology 23, 28–35 (1991). https://doi.org/10.1007/BF01052286

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