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Atypical Acetylcholine Receptors on the Neurons of the Turkish Snail

  • BIOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
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Abstract

Using electrophysiology, the effect of nicotinic acetylcholine receptor (nAChR) ligands on acetylcholine-induced depolarization in the neurons of Helix lucorum snail was studied. It was found that the α-conotoxin PnIA [R9, L10], a selective antagonist of α7 nAChR, and α-cobratoxin (antagonist of α7 and muscle-type nAChR) suppressed neuronal depolarization. Fluorescence microscopy showed staining of the neurons with fluorescently labeled α-bungarotoxin; this staining was reduced by pretreatment with α-cobratoxin. Induced depolarization was also suppressed by α-conotoxin RgIA, a selective inhibitor of α9 nAChR. In contrast to Lymnaea stagnalis nAChR, which are weakly sensitive to neurotoxin II and α-conotoxin GI, antagonists of muscle-type nAChR, H. lucorum receptors were most effectively inhibited by these antagonists. The results obtained, as well as the previously found sensitivity of the receptors studied in this work to muscarinic receptor ligands, indicate an unusual atypical pharmacological profile of H. lucorum nAChR.

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REFERENCES

  1. Kawashima, K., Misawa, H., Moriwaki, Y., et al., Ubiquitous expression of acetylcholine and its biological functions in life forms without nervous systems, Life Sci., 2007, vol. 80, no. 24, pp. 2206–2209. https://doi.org/10.1016/j.lfs.2007.01.059

    Article  CAS  PubMed  Google Scholar 

  2. Papke, R.L., Merging old and new perspectives on nicotinic acetylcholine receptors, Biochem. Pharmacol., 2014, vol. 89, no. 1, pp. 1–11. https://doi.org/10.1016/j.bcp.2014.01.029

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Zoli, M., Pucci, S., Vilella, A., et al., Neuronal and extraneuronal nicotinic acetylcholine receptors, Curr. Neuropharmacol., 2018, vol. 16, no. 4, pp. 338–349. https://doi.org/10.2174/1570159X15666170912110450

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Kanemoto, Y., Ishibashi, H., Doi, A., et al., An electrophysiological study of muscarinic and nicotinic receptors of rat paratracheal ganglion neurons and their inhibition by Z-338, Br. J. Pharmacol., 2002, vol. 135, no. 6, pp. 1403–1414. https://doi.org/10.1038/sj.bjp.0704610

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Finkel, A.S., A cholinergic chloride conductance neurons of Helix aspersa, J. Physiol., 1983, vol. 344, pp. 119–135. https://doi.org/10.1113/jphysiol.1983.sp014928

    Article  CAS  Google Scholar 

  6. Pivovarov, A.S. and Drozdova, E.I., Identification of cholinergic receptors on the soma of PPa3 and LPa3 neurons of the Turkish snail, Neirofiziologiya, 1992, vol. 24, no. 1, pp. 77–86.

    CAS  Google Scholar 

  7. Pivovarov, A.S. and Saganelidze, G.N., Identification of nicotinic and muscarinic cholinergic receptor in the soma of the PPa4 neuron of the Turkish snail, Neirofiziologiya, 1988, vol. 20, no. 2, pp. 203–212.

    CAS  Google Scholar 

  8. Dutertre, S., Nicke, A., and Tsetlin, V.I., Nicotinic acetylcholine receptor inhibitors derived from snake and snail venoms, Neuropharmacology, 2017, vol. 127, pp. 196–223. https://doi.org/10.1016/j.neuropharm.2017.06.011

    Article  CAS  Google Scholar 

  9. Verbitsky, M., Rothlin, C.V., Katz, E., et al., Mixed nicotinic-muscarinic properties of the alpha9 nicotinic cholinergic receptor, Neuropharmacology, 2000, vol. 39, no. 13, pp. 2515–2524.https://doi.org/10.1016/s0028-3908(00)00124-611044723

    Article  CAS  PubMed  Google Scholar 

  10. Vulfius, C.A., Krasts, I.V., Utkin, Y.N., et al., Nicotinic receptors in Lymnaea stagnalis neurons are blocked by alpha-neurotoxins from cobra venoms, Neurosci. Lett., 2001, vol. 309, no. 3, pp. 189–192. https://doi.org/10.1016/s0304-3940(01)02081-x

    Article  CAS  PubMed  Google Scholar 

  11. Utkin, Y.N., Kukhtina, V.V., Kryukova, E.V., et al., “Weak toxin” from Naja kaouthia is a nontoxic antagonist of alpha 7 and muscle-type nicotinic acetylcholine receptors, J. Biol. Chem., 2001, vol. 276, no. 19, pp. 15810–15815. https://doi.org/10.1074/jbc.M100788200

    Article  CAS  PubMed  Google Scholar 

  12. Gerzanich, V., Peng, X., Wang, F., et al., Comparative pharmacology of epibatidine: a potent agonist for neuronal nicotinic acetylcholine receptors, Mol.Pharmacol., 1995, vol. 48, no. 4, pp. 774–782.

    CAS  PubMed  Google Scholar 

  13. Hone, A.J., Servent, D., and McIntosh, J.M., α9-Containing nicotinic acetylcholine receptors and the modulation of pain, Br. J. Pharmacol., 2018, vol. 175, no. 11, pp. 1915–1927. https://doi.org/10.1111/bph.13931

    Article  CAS  PubMed  Google Scholar 

  14. Colquhoun, L., Holden-Dye, L., and Walker, R.J., The pharmacology of cholinoceptors on the somatic muscle cells of the parasitic nematode Ascaris suum,J. Exp. Biol., 1991, vol. 158, pp. 509–530.

    CAS  PubMed  Google Scholar 

  15. Wustenberg, D.G. and Grunewald, B., Pharmacology of the neuronal nicotinic acetylcholine receptor of cultured Kenyon cells of the honeybee, Apis mellifera,J. Comp. Physiol. A: Neuroethol. Sens. Neural Behav. Physiol., 2004, vol. 190, no. 10, pp. 807–821. https://doi.org/10.1007/s00359-004-0530-7

    Article  CAS  Google Scholar 

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Funding

This work was supported by the Russian Foundation for Basic Research (project nos. 17-00-00063 and 17-00-00052).

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Authors and Affiliations

  1. Moscow State University, 119991, Moscow, Russia

    A. S. Pivovarov, T. A. Palikhova, G. M. Nikolaev, A. N. Velikanov & N. A. Vasilieva

  2. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    I. E. Kasheverov, Yu. N. Utkin &  V. I. Tsetlin

Authors
  1. A. S. Pivovarov
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  2. T. A. Palikhova
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  3. G. M. Nikolaev
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  4. A. N. Velikanov
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  5. N. A. Vasilieva
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  6. I. E. Kasheverov
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  7. Yu. N. Utkin
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  8. V. I. Tsetlin
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Corresponding author

Correspondence to Yu. N. Utkin.

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Conflict of interest. The authors declare that they have no conflict of interest.

Statement on the welfare of animals. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Translated by M. Batrukova

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Pivovarov, A.S., Palikhova, T.A., Nikolaev, G.M. et al. Atypical Acetylcholine Receptors on the Neurons of the Turkish Snail. Dokl Biochem Biophys 491, 81–84 (2020). https://doi.org/10.1134/S1607672920020118

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  • DOI: https://doi.org/10.1134/S1607672920020118

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