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The monoclonal antibody, anti-asialoglycophorin from human erythrocytes, specific forβ-d-Gal-1-3-α-d-GalNAc-chains (Thomsen-Friedenreich receptors)

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Abstract

The monoclonal antibody 22.19 of IgM class obtained after immunization of BALB/c mice with asialoglycophorin of human erythrocyte membranes is described. The specificity of this antibody for β-d-Gal-1-3-α-d-GalNAc- disaccharide chains (Thomsen-Friedenreich receptors) was established by studying its reactivity against various erythrocytes, glycoproteins and oligosaccharides and by comparison with two lectins, peanut agglutinin andVicia graminea lectin, which recognize these disaccharide chains.

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Abbreviations

PNA:

peanut agglutinin

VgL:

Vicia graminea lectin

TF:

Thomsen-Friedenreich

HSA:

human serum albumin

MoAb:

monoclonal antibody

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Authors and Affiliations

  1. Department of Tumor Immunology, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Czerska 12, 53-114, Wroclaw, Poland

    Iga Steuden & Czeslaw Radzikowski

  2. Department of Immunochemistry, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Czerska 12, 53-114, Wroclaw, Poland

    Maria Duk, Marcin Czerwiński & Elwira Lisowska

Authors
  1. Iga Steuden
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  2. Maria Duk
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  3. Marcin Czerwiński
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  4. Czeslaw Radzikowski
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  5. Elwira Lisowska
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Steuden, I., Duk, M., Czerwiński, M. et al. The monoclonal antibody, anti-asialoglycophorin from human erythrocytes, specific forβ-d-Gal-1-3-α-d-GalNAc-chains (Thomsen-Friedenreich receptors). Glycoconjugate J 2, 303–314 (1985). https://doi.org/10.1007/BF01049276

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  • DOI: https://doi.org/10.1007/BF01049276

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