Abstract
The binding of five radiolabelled lectins (Vicia graminea, peanut,Phaseolus vulgaris isolectins E-PHA and L-PHA,Evonymus europaeus) to untreated and desialylated K562 cells and human erythrocytes was compared. The number of glycophorin A receptors recognized on the K562 cells by anti-blood group NV. graminea lectin was comparable to that found on the MN or NN erythrocyte surface. However, K562 cells had a severalfold higher number of oligosaccharide chains (presumablyO-glycosidic) which after desialylation became high-affinity receptors for peanut agglutinin, and of complex typeN-glycosidic chains available for the reaction with E-PHA and also with L-PHA (the latter lectin was not bound to erythrocytes). Moreover, K562 cells not treated with neuraminidase had a significant amount of extremely low affinity receptors for peanut agglutinin, whereas binding of this lectin to untreated erythrocytes was undetectable. On the other hand, the untreated K562 cells did not bind anti-blood group B and HE. europaeus lectin, but a small amount of binding by the desialylated cells was observed. Some other differences observed in the mode of lectin binding to K562 cells and erythrocytes are discussed.
Similar content being viewed by others
References
Lozzio BB, Lozzio CB, Ichiki AT, Kalmaz GD, Bamberger EG (1983) in Hematopoietic Stem Cells, eds. Killman SA, Cronkite EP, Muller-Beret CN, Munksgaard, Copenhagen. p. 323–37.
MacDougall SL, Shustik O, Sullivan AK (1983) Cell Immunol 76:143–47.
Andersson LC, Nilsson K, Gahmberg CG (1979) Int J Cancer 23:143–47.
Gahmberg CG, Jokinen M, Andersson LC (1979) J Biol Chem 254:7442–48.
Turco SJ, Rush JS, Jane RA (1980) J Biol Chem 255:3266–69.
Fukuda M (1980) Nature 285:405–7.
Testa U, Henri A, Bettaieb A, Tileux M, Vainchenker W, Tonthat H, Docklear MC, Rochant H (1982) Cancer Res 42:4694–4700.
Kannagi R, Papayannopoulou T, Nakamoto B, Cochran VA, Yokochi T, Stamatoyannopoulos G, Hakomori S (1983) Blood 62:1230–41.
Benz EJ Jr, Murnane MJ, Tonkonow BL, Berman BW, Mazur EM, Cavallesco C, Jenko T, Snyder El, Forget BG, Hoffman R (1980) Proc Natl Acad Sci, USA 77:3509–13.
Horton HA, Cedar SH, Edwards PAW (1981) Scand J Haematol 27:231–40.
Suzuki A, Karol RA, Kundu SK, Marcus DM (1981) Int J Cancer 28:271–76.
Yoshima H, Shiraishi N, Matsumoto A, Maeda B, Sugiyama T, Kobata A (1982) J. Biochem (Tokyo) 91:233–46.
Gerwitz AM, Burger D, Rado TA, Benz EJ, Hoffman R (1982) Blood 60:785–89.
O'Keefe D, Ashman L (1982) Clin Exp Immunol 48:329–38.
Sadler JE, Paulson C, Hill RL (1979) J Biol Chem 254:2112–19.
Jourdian GW, Dean L, Roseman S (1971) J Biol Chem 246:430–35.
Lisowska E, Szeliga W, Duk M (1976) FEBS Lett 72:327–30.
Duk M, Lisowska E (1981) Eur J Biochem 118:131–36.
Starościk K, Waśniowska K, Syper D, Lisowska E (1981) Arch Immunol Ther Exp (Warsz) 31:127–33.
Petryniak J, Janusz M, Markowska E, Lisowska E (1981) Acta Biochim Pol 28:267–73.
Felsted RL, Leavitt RD, Bachur NR (1975) Biochim Biophys Acta 405:72–81.
Leavitt RD, Felsted RL, Bachur RN (1977) J Biol Chem 252:2961–66.
Greenwood FC, Hunter WM, Glover JS (1963) Biochem J 89:114–23.
Duk M, Lisowska E, Kordowicz M, Waśniowska K (1982) Eur J Biochem 123:105–12.
Pereira MEA, Kabat EA, Lotan R, Sharon N (1976) Carbohydr Res 51:107–18.
Cummings RD, Kornfeld S (1982) J Biol Chem 257:11230–34.
Hammarström S, Hammarström ML, Sundblad G, Arnarp J, Lönngren J (1982) Proc Natl Acad Sci, USA 79:1611–15.
Petryniak J, Pereira MEA, Kabat EA (1977) Arch Biochem Biophys 178:118–34.
Prigent MJ, Blanchard D, Cartron JP (1983) Arch Biochem Biophys 222:231–44.
Dahr W, Uhlenbruck G, Janssen E, Schmalisch R (1977) Hum Genet 35:335–43.
Waśniowska K, Drzeniek Z, Lisowska E (1977) Biochem Biophys Res Commun 76:385–90.
Furthmayr H (1978) Nature 271:519–24.
Carter WG, Sharon N (1977) Arch Biochem Biophys 180:570–82.
Egorin MJ, Bachur SM, Felsted RL, Leavitt RD, Bachur NR (1979) J Biol Chem 254:894–98.
Petryniak J, Petryniak B, Waśniowska K, Krotkiewski H (1980) Eur J Biochem 105:335–41.
Yoshima H, Furthmayr H, Kobata A (1980) J Biol Chem 254:3700–03.
Irimura T, Tsuji T, Tagami S, Yamamoto K, Osawa T (1981) Biochemistry 20:560–66.
Prujanski A, Ravid A, Sharon N (1978) Biochim Biophys Acta 508:137–46.
Reisner Y, Lis H, Sharon N (1978) Exp Cell Res 97:445–48.
Ketis NV, Grant CWM (1982) Biochim Biophys Acta 689:194–202.
Duk M, Lisowska E (1984) Eur J Biochem, in press.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Duk, M., Duś, D., Gawlikowski, W. et al. Evaluation of glycoconjugates on the K562 cell surface by means of lectin binding — comparison with human erythrocytes. Glycoconjugate J 1, 81–93 (1984). https://doi.org/10.1007/BF01875415
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01875415