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Evidence from lectin-binding studies for abnormal glycosylation ofβ-hexosaminidase in the leukaemic cell-line CCRF/CEM

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Abstract

The lectin affinities of β-N-acetyl-d-hexosaminidase (E.C.3.2.1.52) from an acute lymphoblastic leukaemic cell-line (CCRF/CEM), a non-malignant lymphoblastic cell-line (SM1) and normal human fibroblasts were studied for both mature and precursor forms of the enzyme. Four immobilised lectins concanavalin A-Sepharose wheat germ agglutinin-Agarose,Ricinus communis agglutinin I-Agarose,Phaseolus vulgaris erythroagglutinin-Agarose and a column of serotonin-Sepharose were used. The activities of β-hexosaminidase from fibroblasts and SM1 cells generally behaved similarly while the CCRF/CEM enzyme exhibited different binding patterns. Differences were also noted between precursor and mature enzyme from each cell type consistent with changes in glycosylation between the precursor form and the mature form appearing in the lysosome. These results suggest that changes in the glycosylation of β-hexosaminidase, and possibly other lysosomal enzymes, may be associated with malignancy.

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Abbreviations

Con A:

concanavalin A-Sepharose

RCA-I:

Ricinus communis agglutinin I-Agarose

WGA:

wheat germ agglutinin-Agarose

PHA-E:

Phaseolus vulgaris erythroagglutinin-Agarose

SER:

serotonin-Sepharose: non-T

non-B ALL:

non-T, non-B cell acute lyphoblastic leukaemia

4-MU-GLcNAc:

4-methylumbelliferyl 2-acetamido-2-deoxy-β-D-glucopyranoside

References

  1. Ellis RB, Rapson NT, Patrick AD, Greaves MF (1978) N Engl J Med 298:476–80.

    Article  CAS  PubMed  Google Scholar 

  2. Besley GTN, Broadhead DM, Bain, AD, Dewar AE, Eden OB (1978) Lancet II:1311.

    Article  Google Scholar 

  3. Tanaka T, Kobayashi M, Saito O, Kamada N, Kuramoto A, Usui T (1981) Clin Chim Acta 117:121–31.

    Article  CAS  PubMed  Google Scholar 

  4. Broadhead DM, Besley GTN, Moss SE, Bain AD, Eden OB Sainsbury CPQ (1981) Leuk Res 5:29–40.

    Article  CAS  PubMed  Google Scholar 

  5. Besley GTN, Moss SE, Bain AD, Dewar AE (1983) J Clin Pathol 36:1000–4.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Drexler HG, Gaedicke G, Minowada J (1984) J Natl Cancer Inst 6:1283–98.

    Google Scholar 

  7. Dewji N, Rapson N, Greaves M, Ellis R (1981) Leuk Res 5:19–27.

    Article  CAS  PubMed  Google Scholar 

  8. Fiddler MB, Ben-Yoseph Y, Nadler HL (1979) Biochem J 177:175–80.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Moss SE, Elder PA, Besley GTN (1985) Dis Markers, in press.

  10. Sturgeon RJ, Sturgeon CM (1982) Carbohydr Res 103:213–19.

    Article  CAS  PubMed  Google Scholar 

  11. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) J Biol Chem 193:265–75.

    CAS  PubMed  Google Scholar 

  12. Deane DL, Cohen BB, Morton JE, Steel CM (1984) Int J Cancer 34:459–62.

    Article  CAS  PubMed  Google Scholar 

  13. Besley GTN, Bain AD (1976) J Med Genet 13:195–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Lisman JJW, Overdijk B (1978) Hoppe-Seylers Z Physiol Chem 359:1019–22.

    CAS  PubMed  Google Scholar 

  15. van Elsen AF, Leroy JG (1979) in Models for the Study of Inborn Errors of Metabolism, ed. Hommes FA, Elsevier, Amsterdam, p 329–32.

    Google Scholar 

  16. Swallow DM, West LF, van Elsen A (1984) Ann Hum Genet 48:215–21.

    Article  CAS  PubMed  Google Scholar 

  17. Joziasse DH, van den Eijnden DH, Lisman JJW, Hooghwinkel GJM (1981) Biochim Biophys Acta 660:174–85.

    Article  CAS  PubMed  Google Scholar 

  18. Overdijk B, van Steijn G, Wolf JH, Lisman JJW (1982) Int J Biochem 14:25–31.

    Article  CAS  PubMed  Google Scholar 

  19. Hasilik A, von Figura K (1981) Eur J Biochem 121:125–9.

    Article  CAS  PubMed  Google Scholar 

  20. Cummings RD, Kornfeld S (1982) J Biol Chem 257:11235–40.

    CAS  PubMed  Google Scholar 

  21. Gallagher JT (1984) Biosci Rep 4:621–32.

    Article  CAS  PubMed  Google Scholar 

  22. Narita M, Taniguchi N, Makita A, Kodama T, Araki E, Oikawa K (1983) Cancer Res 43:5037–42.

    CAS  PubMed  Google Scholar 

  23. Alhadeff JA, Prorok JJ, Dura PA, Plucinsky, MC, Beesburg RY (1984) Cancer Res 44:5422–26.

    CAS  PubMed  Google Scholar 

  24. Miller AL, Kress BC, Stein R, Kinnon C, Kern H, Schneider JA, Harms E (1981) J Biol Chem 256:9352–62.

    CAS  PubMed  Google Scholar 

  25. Vladutiu GD, Rattazzi MC (1978) Biochim Biophys Acta 539:31–36.

    Article  CAS  PubMed  Google Scholar 

  26. Rousson R, Ben-Yoseph Y, Fiddler MB, Nadler HL (1979) Biochem J 180:501–5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Kress BC, Hirani S, Freeze HH, Little L, Miller AL (1982) Biochem J 207:421–28.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Rossowski W, Srivastava BIS (1983) Eur J Cancer Clin Oncol 19:1431–37.

    Article  CAS  PubMed  Google Scholar 

  29. Campbell C, Stanley P (1984) J Biol Chem 261:13370–8.

    Google Scholar 

  30. Overdijk B, van Steijn GJ, Trippelvitz LAW, Lisman JJW, van Halbeek H, Vliegenthart JFG (1983) in Proc 7th Int, Symp Glycoconjugates, eds. Chester MA, Heinegård D, Lundblad A, Svensson S, Secretariat, Lund p 18–9.

    Google Scholar 

  31. Mizuochi T, Nishimura R, Derappe C, Taniguchi T, Hamamoto T, Mochizuki M, Kobata A (1983) J Biol Chem 258:14126–29.

    CAS  PubMed  Google Scholar 

  32. Santer UV, Gilbert F, Glick MC (1984) Cancer Res 44:3730–5.

    CAS  PubMed  Google Scholar 

  33. Berger EG, Buddecke, E, Kamerling JP, Kobata A, Paulson JC, Vliegenthart JFG (1982) Experientia 38:1129–258.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pathology, Royal Hospital for Sick Children and University of Edinburgh, EH9 1LF, Edinburgh, UK

    Stephen E Moss & Guy T N Besley

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  1. Stephen E Moss
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  2. Guy T N Besley
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Moss, S.E., Besley, G.T.N. Evidence from lectin-binding studies for abnormal glycosylation ofβ-hexosaminidase in the leukaemic cell-line CCRF/CEM. Glycoconjugate J 2, 267–277 (1985). https://doi.org/10.1007/BF01049273

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  • DOI: https://doi.org/10.1007/BF01049273

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