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Methode zur Ochratoxin-A-Bestimmung in Lebens- und Futtermitteln mittels Hochdruckflüssigkeitschromatographie (HPLC)

Method for quantitative determination of ochratoxin A in foods and feeds by means of High-pressure Liquid Chromatography (HPLC)

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Summary

A rapid, specific HPLC-method has been developed for quantitative determination of Ochratoxin A in food and feedstuffs as well as in mixed-feed.

After preliminary clean-up of the McOH/Chloroform extract the ochratoxin is analyzed using HPLC with a Spherisorb S5 ODS column McOH/water/acetic acid as eluent and a fluorescence detector.

The average recovery was about 50% in the range for 50–500 ppb with a small variation (coefficient of variation 10.24% and 23.650. The detection limit for Ochratoxin A is 5 ppb.

Zusammenfassung

Eine schnelle, spezifische HPLC-Methode zum quantitativen Nachweis von Ochratoxin A sowohl in Lebens- und Futtermitteln als auch im Mischfutter wird vorgestellt.

Ochratoxin A wird nach Vorreinigung im Schütteltrichter durch die mobile Phase Methanol/dest. Wasser (70+30)+1%. Essigsäure 100% von der Spherisorb S5 ODS-HPLC-Trennsäule eluiert und im Fluorescenzdetektor nachgewiesen.

Im Bereich von 50 und 500 ppb weisen die mittleren Auffindungsraten rund 50%, bei nur geringer Schwankungsbreite (Variationskoeffizient 10,24% und 23,650 auf. 5 ppb können eindeutig erfaßt werden.

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Authors and Affiliations

  1. Institut für Ernährung, Vet.-Med. Univ. Wien, Wien

    Hans Schweighardt, Josef Böhm & Josef Leibetseder

  2. IL Med. Univ.-Klinik für Klauentiere, Vet.-Med. Univ. Wien, Linke Bahngasse 11, A-1030, Wien, Österreich

    Maximilian Schuh

  3. Angehöriger des Institutes für Tierproduktion, Fakultät für Bodenkultur, Universität Mansoura, Mansoura, Ägypten

    Abdelhamid Mohamed Abdelhamid

Authors
  1. Hans Schweighardt
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  2. Maximilian Schuh
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  3. Abdelhamid Mohamed Abdelhamid
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  4. Josef Böhm
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  5. Josef Leibetseder
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Schweighardt, H., Schuh, M., Abdelhamid, A.M. et al. Methode zur Ochratoxin-A-Bestimmung in Lebens- und Futtermitteln mittels Hochdruckflüssigkeitschromatographie (HPLC). Z Lebensm Unters Forch 170, 355–359 (1980). https://doi.org/10.1007/BF01042973

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  • DOI: https://doi.org/10.1007/BF01042973

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