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Analytik von Gangliosiden unter besonderer Berücksichtigung der Milch

Analysis of gangliosides with particular reference to milk

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Abstract

Gangliosides are sialic acid containing lipid compounds of a very complex nature, which are involved in many physiological processes. Gangliosides are very important constituents of membrane material in animal tissue, where they occur at very low concentrations. Improved analytical procedures are therefore required to study their behaviour, their role in living systems and their isolation for further investigations. In this paper, all analytical methods which may be important in the analysis of gangliosides are summarized and discussed. These include extraction, purification, enrichment and chromatographic procedures. In bovine milk, three gangliosides of known structure are present. Their isolation, structure and analytical importance (buttermilk) are also reported.

Zusammenfassung

Ganglioside sind Sialinsäure enthaltende Lipide (N-Acetylneuraminsäure), die als funktionell wichtige Bausteine besonders in Membranen anzutreffen sind. Sie spielen eine wichtige Rolle in vielen elementaren physiologischen Vorgängen in tierischem Gewebe. Um ihre weite Bedeutung näher zu untersuchen bzw. die komplexe Struktur zu charakterisieren, sind aufwendige analytische Verfahren notwendig, die in dieser kurzen Übersicht dargestellt werden, d. h. Extraktion, Reinigung und Anreicherung Bowie chromatographische Methoden. In der Milch (Milchfettkügelchenmembran) wurden bisher drei Ganglioside in ihrer Struktur aufgeklärt. Sie werden bei der Verbutterung in der Buttermilch angereichert und könnten so als wichtiges analytisches Hilfsmittel dienen.

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  1. Lehrstuhl für Lebensmittelchemie und -technologie der Bergischen Universität, Gesamthochschule Wuppertal, Postfach 100 127, D-5600, Wuppertal 1, Bundesrepublik Deutschland

    Uwe Dirks & Ernst H. Reimerdes

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  1. Uwe Dirks
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Dirks, U., Reimerdes, E.H. Analytik von Gangliosiden unter besonderer Berücksichtigung der Milch. Z Lebensm Unters Forch 186, 99–107 (1988). https://doi.org/10.1007/BF01042701

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