Abstract
In this chapter, we present the preparation of gangliosides isotopically labelled with 3H or 14C. The methods do not present specific difficulties and can be used in any radiochemical laboratory. Some procedures can be applied to both gangliosides and neutral glycosphingolipids.
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References
Chigorno V, Tettamanti G, Sonnino S (1996) Metabolic processing of gangliosides by normal and Salla human fibroblasts in culture. A study performed by administering radioactive GM3 ganglioside. J Biol Chem 271(36):21738–21744
Radsak K, Schwarzmann G, Wiegandt H (1982) Studies on the cell association of exogenously added sialo-glycolipids. Hoppe-Seyler's Zeitschrift fur physiologische Chemie 363(3):263–272
Schwarzmann G, Hoffmann-Bleihauer P, Schubert J, Sandhoff K, Marsh D (1983) Incorporation of ganglioside analogues into fibroblast cell membranes. A spin-label study. Biochemistry 22(21):5041–5048
Chigorno V, Pitto M, Cardace G, Acquotti D, Kirschner G, Sonnino S, Ghidoni R, Tettamanti G (1985) Association of gangliosides to fibroblasts in culture: a study performed with GM1 [14C]-labelled at the sialic acid acetyl group. Glycoconj J V2(3):279–291
Orlando P, Cocciante G, Ippolito G, Massari P, Roberti S, Tettamanti G (1979) The fate of tritium labeled GM1 ganglioside injected in mice. Pharmacol Res Commun 11(9):759–773
Trinchera M, Ghidoni R, Sonnino S, Tettamanti G (1990) Recycling of glucosylceramide and sphingosine for the biosynthesis of gangliosides and sphingomyelin in rat liver. Biochem J 270(3):815–820
Ghidoni R, Tettamanti G, Zambotti V (1977) Labeling of natural substrates for the radiochemical assay of enzymes involved in lipid storage diseases: a general procedure for tritiation of gangliosides. Biochem Exp Biol 13(1):61–69
Leskawa KC, Dasgupta S, Chien JL, Hogan EL (1984) A simplified procedure for the preparation of tritiated GM1 ganglioside and other glycosphingolipids. Anal Biochem 140(1):172–177
Fra AM, Masserini M, Palestini P, Sonnino S, Simons K (1995) A photo-reactive derivative of ganglioside GM1 specifically cross-links VIP21-caveolin on the cell surface. FEBS Lett 375(1–2):11–14
Sonnino S, Cantu L, Corti M, Acquotti D, Venerando B (1994) Aggregative properties of gangliosides in solution. Chem Phys Lipids 71(1):21–45
Sonnino S, Acquotti D, Kirschner G, Uguaglianza A, Zecca L, Rubino F, Tettamanti G (1992) Preparation of lyso-GM1 (II3Neu5AcGgOse4-long chain bases) by a one-pot reaction. J Lipid Res 33(8):1221–1226
Taketomi T, Kawamura N (1970) Preparation of lysohematoside from hematoside of equine erythrocyte and its chemical and hemolytic properties. J Biochem 68(4):475–485
Tayot JL, Tardy M (1980) Isolation of cholera toxin by affinity chromatography on porous silica beads with covalently coupled ganglioside GM1. Adv Exp Med Biol 125:471–478
Higashi H, Basu S (1982) Specific 14C labeling of sialic acid and N-acetylhexosamine residues of glycosphingolipids after hydrazinolysis. Anal Biochem 120(1):159–164
Neuenhofer S, Schwarzmann G, Egge H, Sandhoff K (1985) Synthesis of lysogangliosides. Biochemistry 24(2):525–532
Sonnino S, Kirschner G, Ghidoni R, Acquotti D, Tettamanti G (1985) Preparation of GM1 ganglioside molecular species having homogeneous fatty acid and long chain base moieties. J Lipid Res 26(2):248–257
Ghidoni R, Sonnino S, Masserini M, Orlando P, Tettamanti G (1981) Specific tritium labeling of gangliosides at the 3-position of sphingosines. J Lipid Res 22(8):1286–1295
Sonnino S, Ghidoni R, Gazzotti G, Kirschner G, Galli G, Tettamanti G (1984) High performance liquid chromatography preparation of the molecular species of GM1 and GD1a gangliosides with homogeneous long chain base composition. J Lipid Res 25(6):620–629
Gazzotti G, Sonnino S, Ghidoni R, Orlando P, Tettamanti G (1984) Preparation of the tritiated molecular forms of gangliosides with homogeneous long chain base composition. Glycoconj J 1(2):111–121. https://doi.org/10.1007/bf01213725
Negroni E, Chigorno V, Tettamanti G, Sonnino S (1996) Evaluation of the efficiency of an assay procedure for gangliosides in human serum. Glycoconj J 13(3):347–352
Schwarzmann G (1978) A simple and novel method for tritium labeling of gangliosides and other sphingolipids. Biochim Biophys Acta 529(1):106–114
Valiente O, Mauri L, Casellato R, Fernandez LE, Sonnino S (2001) Preparation of deacetyl-, lyso-, and deacetyl-lyso-GM(3) by selective alkaline hydrolysis of GM3 ganglioside. J Lipid Res 42(8):1318–1324
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Mauri, L., Prioni, S., Ciampa, M.G., Sonnino, S. (2018). Radioactive Gangliosides for Biological Studies. In: Sonnino, S., Prinetti, A. (eds) Gangliosides. Methods in Molecular Biology, vol 1804. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8552-4_15
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DOI: https://doi.org/10.1007/978-1-4939-8552-4_15
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