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A novel oligonucleotide cassette for the overproduction ofEscherichia coli aspartate transcarbamylase inBacillus stearothermophilus

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Summary

A novel double-stranded oligonucleotide cassette was devised in order to express heterologous genes inBacillus stearothermophilus. By linking the cassette to the upstream ofEscherichia coli aspartate transcarbamylase gene, this enzyme was overexpressed inB. stearothermophilus cells.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, Utsunomiya University, 350 Minemachi, 321, Utsunomiya, Tochigi, Japan

    Issay Narumi, Kazumi Sawakami, Toshiyuki Kimura & Tadashi Yanagisawa

  2. NEC corporation, 4-1-1 Miyazaki, Miyamae-ku, 216, Kawasaki, Kanagawa, Japan

    Shinya Nakamoto & Noriyuki Nakayama

  3. The Institute of Physical and Chemical Research, 2-1 Hirosawa, 351-01, Wako, Saitama, Japan

    Nobutaka Takahashi

  4. Jichi Medical School, School of Nursing, 3311-159 Yakushiji, 329-04, Minamikawachi, Tochigi, Japan

    Hiroshi Kihara

Authors
  1. Issay Narumi
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  2. Kazumi Sawakami
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  3. Toshiyuki Kimura
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  4. Shinya Nakamoto
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  5. Noriyuki Nakayama
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  6. Tadashi Yanagisawa
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  7. Nobutaka Takahashi
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  8. Hiroshi Kihara
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Narumi, I., Sawakami, K., Kimura, T. et al. A novel oligonucleotide cassette for the overproduction ofEscherichia coli aspartate transcarbamylase inBacillus stearothermophilus . Biotechnol Lett 14, 759–764 (1992). https://doi.org/10.1007/BF01029134

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  • DOI: https://doi.org/10.1007/BF01029134

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