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Biotechnology Letters

, Volume 14, Issue 9, pp 759–764 | Cite as

A novel oligonucleotide cassette for the overproduction ofEscherichia coli aspartate transcarbamylase inBacillus stearothermophilus

  • Issay Narumi
  • Kazumi Sawakami
  • Toshiyuki Kimura
  • Shinya Nakamoto
  • Noriyuki Nakayama
  • Tadashi Yanagisawa
  • Nobutaka Takahashi
  • Hiroshi Kihara
Article

Summary

A novel double-stranded oligonucleotide cassette was devised in order to express heterologous genes inBacillus stearothermophilus. By linking the cassette to the upstream ofEscherichia coli aspartate transcarbamylase gene, this enzyme was overexpressed inB. stearothermophilus cells.

Keywords

Enzyme Aspartate Organic Chemistry Bioorganic Chemistry Heterologous Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bradford, M. M. (1976)Anal. Biochem. 72, 248–254.Google Scholar
  2. Brückner, R., Zyprian, E., and Matzura, H. (1984)Gene 32, 151–160.Google Scholar
  3. Davis, B. J. (1964).Ann. N. Y. Acad. Sci. 121, 404–427.Google Scholar
  4. Dower, W. J., Miller, J. F., and Ragsdale, C. W. (1988)Nucl. Acids Res. 16, 6127–6145.Google Scholar
  5. Ehrlich, S. D. (1978)Proc. Natl. Acad. Sci. U.S.A. 75, 1433–1436.Google Scholar
  6. Fujii, M., Imanaka, T., and Aiba, S. (1982).J. Gen. Microbiol. 128, 2997–3000.Google Scholar
  7. Gerhart, J. C., and Pardee, A. B. (1961).J. Biol. Chem. 237, 891–896.Google Scholar
  8. Gerhart, J. C., and Holoubek, H. (1967).J. Biol. Chem. 242 2886–2892.Google Scholar
  9. Gold, L., Pribnow, D., Schneider, T., Shinedling, S., Singer, B. S., and Stormo, G. (1981)Annu. Rev. Microbiol. 35, 365–403.Google Scholar
  10. Imanaka, T., Fujii, M., Aramori, I., and Aiba, S. (1982).J. Bacteriol. 149, 824–830.Google Scholar
  11. Kreft, J., Bernhard, K., and Goebel, W. (1978)Mol. Gen. Genet. 162, 59–67.Google Scholar
  12. Laemmli, U. K. (1970)Nature (London) 227, 680–685.Google Scholar
  13. Liao, H., McKenzie, T., and Hageman, R. (1986)Proc. Natl. Acad. Sci. U.S.A. 83, 576–580.Google Scholar
  14. Loprasert, S., Negoro, S., and Okada, H. (1989)J. Bacteriol. 171 4871–4875.Google Scholar
  15. Matsumura, M., and Aiba, S. (1985)J. Biol. Chem. 260, 15298–15303.Google Scholar
  16. Moran, C. P., Lang, N., LeGrice, S. F. J., Lee, G., Stephens, M., Sonenshein, A. L., Pero, J., and Losick, R. (1982)Mol. Gen. Genet. 186, 339–346.Google Scholar
  17. Nakajima, R., Imanaka, T., and Aiba, S. (1985)J. Bacteriol. 163, 401–406.Google Scholar
  18. Nakamura, K., and Imanaka, T. (1989)Appl. Environ. Microbiol. 55, 3208–3213.Google Scholar
  19. Narumi, I., Sawakami, K., Nakamoto, S., Nakayama, N., Yanagisawa, T., Takahashi, N., and Kihara, H. (1992)Biotechnol. Techniques 6, 83–86.Google Scholar
  20. Pastra-Landis, S. C., Foote, J., and Kantrowitz, E. R. (1981)Anal. Biochem. 118, 358–363.Google Scholar
  21. Pauza, C. D., Karels, M. J., Narve, M., and Schachman, H. K. (1982)Proc. Natl. Acad. Sci. U.S.A. 79, 4020–4024.Google Scholar
  22. Roland, K. L., Powell, F. E., and Turnbough, C. L. (1985)J. Bacteriol. 163, 991–999.Google Scholar
  23. Roof, W. D., Foltermann, K. F., and Wild, J. R. (1982)Mol. Gen. Genet. 187, 391–400.Google Scholar
  24. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989)Molecular Cloning: A Laboratory Manual 2nd ed., N. Y.: Cold Spring Harbor Laboratory Press.Google Scholar
  25. Seibenlist, U., Simpson, R. B., and Gilbert, W. (1980)Cell 20, 269–281.Google Scholar
  26. Soutschek-Bauer, E., and Staudenbauer, W. L. (1987)Mol. Gen. Genet. 208, 537–541.Google Scholar
  27. Sprague, K., Steitz, J., Grenley, R., and Stocking, C. (1977)Nature (London) 267, 462–465.Google Scholar
  28. Takagi, M., Imanaka, T., and Aiba, S. (1985)J. Bacteriol. 163, 824–831.Google Scholar
  29. Tinoco, I., Borer, P. N., Dengler, B., Levine, M. D., Uhlenbeck, O. C., Crothers, D. M. and Gralla, J. (1973)Nature New Biol. 246, 40–41.Google Scholar
  30. Turnbough, C. L., Hicks K. L., and Donahue, J. P. (1983)Proc. Natl. Acad. Sci. U.S.A. 80, 368–372.Google Scholar
  31. Van Charldorp, R., Van, Kimmenade, A. M. A., and Van Knippenberg, P. H. (1981)Nucl. Acids Res. 9, 4909–4917.Google Scholar
  32. Weber, K. (1968)Nature (London) 218, 1116–1119.Google Scholar
  33. Wiley, D. C., and Lipscomb, W. N. (1968)Nature (London) 218, 1119–1121.Google Scholar
  34. Wu, L., and Welker, N. E. (1989)J. Gen. Microbiol. 135, 1315–1324.Google Scholar
  35. Zhang, M., Nakai, H., and Imanaka, T. (1988)Appl. Environ. Microbiol. 54 3162–3164.Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Issay Narumi
    • 1
  • Kazumi Sawakami
    • 1
  • Toshiyuki Kimura
    • 1
  • Shinya Nakamoto
    • 2
  • Noriyuki Nakayama
    • 2
  • Tadashi Yanagisawa
    • 1
  • Nobutaka Takahashi
    • 3
  • Hiroshi Kihara
    • 4
  1. 1.Department of Agricultural ChemistryUtsunomiya UniversityUtsunomiya, TochigiJapan
  2. 2.NEC corporationKawasaki, KanagawaJapan
  3. 3.The Institute of Physical and Chemical ResearchWako, SaitamaJapan
  4. 4.Jichi Medical School, School of NursingMinamikawachi, TochigiJapan

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