Biotechnology Letters

, Volume 14, Issue 9, pp 759–764 | Cite as

A novel oligonucleotide cassette for the overproduction ofEscherichia coli aspartate transcarbamylase inBacillus stearothermophilus

  • Issay Narumi
  • Kazumi Sawakami
  • Toshiyuki Kimura
  • Shinya Nakamoto
  • Noriyuki Nakayama
  • Tadashi Yanagisawa
  • Nobutaka Takahashi
  • Hiroshi Kihara


A novel double-stranded oligonucleotide cassette was devised in order to express heterologous genes inBacillus stearothermophilus. By linking the cassette to the upstream ofEscherichia coli aspartate transcarbamylase gene, this enzyme was overexpressed inB. stearothermophilus cells.


Enzyme Aspartate Organic Chemistry Bioorganic Chemistry Heterologous Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Issay Narumi
    • 1
  • Kazumi Sawakami
    • 1
  • Toshiyuki Kimura
    • 1
  • Shinya Nakamoto
    • 2
  • Noriyuki Nakayama
    • 2
  • Tadashi Yanagisawa
    • 1
  • Nobutaka Takahashi
    • 3
  • Hiroshi Kihara
    • 4
  1. 1.Department of Agricultural ChemistryUtsunomiya UniversityUtsunomiya, TochigiJapan
  2. 2.NEC corporationKawasaki, KanagawaJapan
  3. 3.The Institute of Physical and Chemical ResearchWako, SaitamaJapan
  4. 4.Jichi Medical School, School of NursingMinamikawachi, TochigiJapan

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