Summary
In thepars intermedia ofRana esculenta, two zones may be distinguished: an external zone with normal glandular cells and an internal zone with large globular cells.
The innervation is assumed by a hypothalamic nervous tract which divides in two distinct bundles: The first group of terminals innervates the capillaries in the rostro-ventral corner of thepars intermedia. The second one penetrates the glandular parenchyma where they are numerous in the internal zone and less numerous in the external zone.
On the basis of their morphological and cytological characteristics three kinds of fibres are distinguished: neurosecretory axones with granules of about 1,000 to 1,800 Å in diameter (type 1) terminals with fine granules of 550 to 950 Å, (type 2) and terminals without granules (type 3).
After injection of DL noradrenaline H3 and autoradiography on electron microscopy, the type 2 terminals with fine granules are strongly labeled while type 1 and type 3 fibres are devoid of labeling. The fixation of DL noradrenaline H3 demonstrates the aminergic fibres the function of which is discussed.
Résumé
L'hypophyse intermédiaire deRana esculenta, se compose de deux zones distinctes: une zone externe formée de cellules glandulaires d'aspect normal, une zone interne constituée de cellules à globules.
Son innervation est assurée par un faisceau d'axones d'origine hypothalamique qui se subdivise dans l'angle rostro-ventral en deux groupes de fibres distinctes. Le premier groupe se termine sur les capillaires de cette zone, les fibres du deuxième groupe se terminent au contact des cellules glandulaires. Elles sont nombreuses dans la zone interne, moins abondantes dans la zone externe. D'après leurs caractères morphologiques et cytologiques, on distingue 3 types de fibres: des axones neurosécréteurs à grains d'environ 1000 à 1800 Å de diamètre (type 1), des terminaisons à grains de 550 à 950 Å de diamètre (type 2) et des terminaisons pratiquement dépourvues de grains (type 3).
Après l'injection de DL noradrénaline H3 et étude autoradiographique au microscope électronique, les terminaisons de type 2 à fines granulations se marquent intensément tandis que les fibres du type 1 et du type 3 ne présentent pas de marquage. Cette méthode met en évidence les fibres aminergiques dont le rôle est discuté.
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Avec la collaboration technique de Mme Y. Rohr et de M. C. Chevalier.
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Doerr-Schott, J., Follenius, E. Innervation de l'hypophyse intermédiaire deRana esculenta, et identification des fibres aminergiques par autoradiographie au microscope électronique. Z.Zellforsch 106, 99–118 (1970). https://doi.org/10.1007/BF01027720
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DOI: https://doi.org/10.1007/BF01027720