Skip to main content
SpringerLink
Log in

Influence of the nitrogen source on growth and ethanol production byPichia stipitis NRRL Y-7124

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Growth and ethanol production byPichia stipitis in media containing different nitrogen sources were compared. Ammonium sulphate (plus vitamins and trace elements) increased ethanol productivity 57% and ethanol-biomass yield 113%, compared to yeast extract. Consumption of ammoniu, amino-acids and total nitrogen was determined. Two ammonium effects, low uptake in complex media and fermentation stimulation in defined media are discussed in terms of ammonium- ammonia futile cycling and maintenance energy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Borst Pauwels GWFH (1981)Biochem. Biophys. Acta 650: 88–127.

    Google Scholar 

  • Bridson EY & Brecker A (1970) Design of media. In:Methods in Microbiology, Norris JR & & Ribbons DW, eds. Vol3A pp 255–256. Academic Press, London.

    Google Scholar 

  • Buurman ET, Texeira de Mattos JM & Neijssel OM (1989).FEMS Microbiol. Lett. 58: 229–232.

    Google Scholar 

  • Chaney AL & Marbach EP (1962)Clin. Chem. 8: 130–132.

    Google Scholar 

  • Groojten DRJ, van der Lans RGM & Luyben KCh (1990)Enzyme Microb. Technol. 12: 20–23.

    Google Scholar 

  • Guebel DV, Cordenons A, Nudel BC & Giulietti AM (1991a)J. Ind. Microbiol. 7: 287–292.

    Google Scholar 

  • Guebel DV, Nudel BC & Giulietti AM (1991b)Biotechnol. Techniques, 5: 427–430.

    Google Scholar 

  • Jones RP, Pamment N & Greenfield PF (1981)Process Biochem. Apr–May, 42–49.

  • Jones RP, & Gadd (1990)Enzyme Microb. Technol. 12: 402–418.

    Google Scholar 

  • Kleiner D (1985)FEBS 187: 237–239.

    Google Scholar 

  • Lison L (1968) In: Statistique appliqué a la biologie experimentale pp 115–122 Gautier-Villars, Paris.

    Google Scholar 

  • Peña A, Pardo JP & Ramirez J (1987)Arch. Biochem. Biophys. 253: 431–438.

    Google Scholar 

  • Prior BA, Kilian SG & duPressz JCh (1989)Process Biochem. Feb:21–32.

  • Rao P & Pattabiraman TN (1989)Anal. Biochem. 181: 18–22.

    Google Scholar 

  • Roels JA (1983) In:Energetics, and Kinetics in Biotechnology pp 30–31 Elsevier Science Publishers, The Netherlands.

    Google Scholar 

  • Slavik J (1982)FEBS Lett. 140: 22–25.

    Google Scholar 

  • Slininger PJ, Bolen PL & Kurtzman CP (1987)Enzyme Microb. Technol. 9: 5–15.

    Google Scholar 

  • Soumalainen H & Oura E (1975) In:The Yeast, Rose AH & Harrison JS, eds, Vol 2 pp 37–67 Academic Press, London.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Cátedra de Biotecnología. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956. (1113) Capital, Buenos Aires, Argentina

    Daniel V. Guebel, Alfredo Cordenons, Osvaldo Cascone, Ana M. Giulietti & Clara Nudel

Authors
  1. Daniel V. Guebel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alfredo Cordenons
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Osvaldo Cascone
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ana M. Giulietti
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Clara Nudel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guebel, D.V., Cordenons, A., Cascone, O. et al. Influence of the nitrogen source on growth and ethanol production byPichia stipitis NRRL Y-7124. Biotechnol Lett 14, 1193–1198 (1992). https://doi.org/10.1007/BF01027027

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01027027

Keywords

Search

Navigation