Skip to main content
SpringerLink
Log in

Enhancement of oxygen transfer in Hybridoma cell culture by using a perfluorocarbon as an oxygen carrier

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

In order to increase the oxygen transfer in a bioreactor for Hybridoma cell culture, a perfluorocarbon, FlutecR ppll was used in a modified CelligenTM system. There was no harmful effect of ppll on the cell growth and on the production of monoclonal antibody.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Adlercreutz, P., and Mattiasson, B. (1982)Eur. J. Appl. Microbiol. Biotechnol. 16, 165–170.

    Google Scholar 

  • Boraston, R., Thompson, P.W., Garland, S., and Birch, J.R. (1984)Develop. Biol. Stand.,55, 103–111

    Google Scholar 

  • Brink, L.E.S., and Tramper, J. (1985)Biotech. Bioeng. 27, 1258–1269.

    Google Scholar 

  • Bucala, R., Kawakami, M., and Cerami, A. (1983)Science 220, 965–967.

    Google Scholar 

  • Chandler, D., Davey, M.R., Lowe, K.C., and Mulligan, B.J. (1987)Bioechnol. Letters 9(3), 195–200.

    Google Scholar 

  • Chibata, I., Yamada, S., Wada, M., Izuo, N., and Yamaguchi, T. (1974)U.S. Patent No. 3 850753.

    Google Scholar 

  • Cho, M.H. (1988)Ph. D. Thesis, Rutgers University, New Brunswick, N.J.,

  • Damiano, D. and Wang, S.S. (1985)Biotechnol. Letters 7(2), 81–86.

    Google Scholar 

  • Glacken, M.W., Fleischaker, R.J., and Sinskey, A.J. (1983)Trends in Biotechnology 1(4), 102–108.

    Google Scholar 

  • Hertl, W., and Ramsay, W.S. (1979)U.S. Patent No. 4 166 006.

  • Lowe, K.C., Mcnaughton, D.C., and Moore, J.P. (1984)Brit. J. Pharmac. 82, 276P.

    Google Scholar 

  • Martin, N., Brennan, A., Denome, L., and Shaevitz, J. (1987)Bio/Technology 5. 838–840.

    Google Scholar 

  • Mattiasson, B., and Adlercreutz, P. (1983)Annal N.Y. Acad. Sci. 413, 545–546.

    Google Scholar 

  • Mattiasson, B., and Adlercreutz, P. (1987)Trends in Biotechnology 5, 250–254.

    Google Scholar 

  • Patterson, M.K., Jr. (1979).Method. Enzymol,58, 141

    Google Scholar 

  • Reuveny, S., Zheng, Z.-B., and Eppstein, L. (1986)American Biotechnol. Lab. Jan./Feb. 28–36.

    Google Scholar 

  • Spier, R.E., and Griffiths, B. (1984)Develop. bio. Standard 55, 81–92.

    Google Scholar 

  • Tyo, M.A., and Spier, R.E. (1987)Enzyme Microb. Technol. 9, 514–520.

    Google Scholar 

  • Wise, W.S. (1951)J. Gen. Microbiol. 5, 167–177.

    Google Scholar 

Download references

Author information

Author notes

  1. Moo H. Cho

    Present address: Department of Chemical Engineering, Yeungnam University, Gyongean, Korea

Authors and Affiliations

  1. Department of Chemical and Biochemical Engineering, Rutgers, The State University of New Jersey, PO BOX 909, 08854, Piscataway, NJ, USA

    Moo H. Cho & Shaw S. Wang

Authors
  1. Moo H. Cho
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shaw S. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cho, M.H., Wang, S.S. Enhancement of oxygen transfer in Hybridoma cell culture by using a perfluorocarbon as an oxygen carrier. Biotechnol Lett 10, 855–860 (1988). https://doi.org/10.1007/BF01026995

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01026995

Keywords

Search

Navigation