Summary
Suspension cultures ofCinchona ledgeriana Moens have been developed which exhibit good growth in shake flasks with dry weight yields of approximately 9.0 g.l−1. Cultures have been scaled up for growth in a 7 l air-lift bioreactor. A typical growth curve in the fermenter is shown with similar growth rates but a reduced biomass levels when compared to shake flasks. The analysis of both flask and bioreactor grown suspension cultures indicated the presence of quinidine and low levels of quinine.
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Allan, E.J., Scragg, A.H. Comparison of the growth ofCinchona ledgeriana Moens suspension cultures in shake flasks and 7 litre air-lift bioreactors. Biotechnol Lett 8, 635–638 (1986). https://doi.org/10.1007/BF01025970
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DOI: https://doi.org/10.1007/BF01025970