Summary
The binding of ingested kidney bean (Phaseolus vulgaris) lectins to the luminal surface of the rat gut was investigated by an indirect immunofluorescence method in which the primary antiserum was monospecific for kidney bean globulin lectins. The major lectin-positive sites were found to be the non-crypt regions of villi in the proximal region of the small intestine; exactly the same regions displayed extensive disruption of microvilli. Lectins were not localized in the brush border regions of ileal villi and no microvillus abnormalities were detected in this region of the gut. The luminal surface of the caecum was strongly lectin-positive and here also microvillus disruption was evident. Low levels of lectins were detected on the luminal surface of the colon; no microvillus abnormalities were detected in this region.
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References
Brady, P. G., Vannier, A. M. &Banwell, J. G. (1978) Identification of the dietary lectin, wheat germ agglutinin, in human intestinal contents.Gastroenterology 75, 236–9.
Essner, E., Schreiber, J. &Griewski, R. A. (1978) Localization of carbohydrate components in rat colon with fluoresceinated lectins.J. Histochem. Cytochem. 26, 452–8.
Etzler, M. &Branstrator, M. L. (1974) Differential localization of cell surface and secretory components in rat intestinal epithelium by use of lectins.J. Cell Biol.. 62, 329–43.
Freeman, H. J., Etzler, M. E., Garrido, A. B. &Kim, Y. S. (1978) Alterations in cell surface membrane components of adapting rat small intestinal epithelium. Studies with lectins after massive proximal jejunoileal resection and jejunoileal transposition.Gastroenterology 75, 1066–72.
King, T. P., Pusztai, A. & Clarke, E. M. W. (1980) Kidney bean (Phaseolus vulgaris) lectin induced lesions in rat small intestine.J. comp. Path. Ther. 90 (in press).
Miller, D. S. &Bender, A. E. (1955) The determination of the net utilisation of protein by a shortened method.Br. J. Nutr. 9, 382–8.
Pusztai, A. &Palmer, R. (1977) Nutritional evaluation of kidney beans (Phaseolus vulgaris): the toxic principle.J. Sci. Fd Agric. 28, 620–3.
Pusztai, A. &Stewart, J. C. (1978) Isolectins ofPhaseolus vulgaris, physicochemical studies.Biochim. biophys. Acta 536, 38–49.
Pusztai, A. &Watt, W. B. (1974) Isolectins ofPhaseolus vulgaris, a comprehensive study of fractionation.Biochim. biophys. Acta 365, 57–71.
Pusztai, A., Grant, G. &Palmer, R. (1975) Nutritional evaluation of kidney beans (Phaseolus vulgaris): the isolation and partial characterisation of toxic constituents.J. Sci. Fd Agric. 26, 149–56.
Roth, J. (1978) Compensatory membrane biogenesis and exocytosis as a result of concanavalin A-induced membrane internalization.Exp. Cell Res. 114, 31–8.
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King, T.P., Pusztai, A. & Clarke, E.M.W. Immunocytochemical localization of ingested kidney bean (Phaseolus vulgaris) lectins in rat gut. Histochem J 12, 201–208 (1980). https://doi.org/10.1007/BF01024550
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DOI: https://doi.org/10.1007/BF01024550