Summary
Substance P-like immunoreactivity in the alimentary canal of the frogRana esculenta L. was studied by means of the indirect immunoperoxidase method. In all segments of the gastrointestinal tract, immunoreactivity was revealed in both the myenteric and the submucosa plexus. Stained nerve cells were observed in the myenteric plexus but not in the submucous plexus. The proximal part of the oesophagus and hindgut were free of immunoreactive perkarya. The stained nerve cells were of the Dogiel type I category in the foregut, and type II in the midgut. Both the musculature and gastrointestinal glands were supplied with immune-positive fibres. These results indicate that substance P may play similar roles in the frog gut, as described previously in mammals and fish.
Similar content being viewed by others
References
Barthó, L. &Holzer, P. (1985) Search for a physiological role of substance Pin gastrointestinal motility.Neuroscience 16, 1–32.
Barthó, L., Holzer, P., Donnerer, J. &Lembeck, F. (1982) Effects of substance P, cholecystokinin octapeptide, bombesin and neurotensin on the peristaltic reflex of the guinea-pig ileum in the absence and in the presence of atropin.Naunyn-Schniedibergs arch. Pharmac. 321, 321–8.
Bjenning, C. &Holmgern, S. (1983) Neuropeptides in the fish gut.Histochemistry 88, 156–63.
Costa, M., Furness, J. B., Llewellyn-Smith, I. J. &Cuello, A. C. (1981) Projections of substance P-containing neurons within the guinea-pig small intestine.Neuroscience 5, 411–24.
Furness, J. B. &Costa, M. (1987)The enteric nervous system. London: Churchill Livingstone.
Furness, J. B., Costa, M. &Keast, J. R. (1984) Cholineacetyltransferase and peptide immunoreactivity of submucous neurons in the small intestine of guinea-pig.Cell Tissue Res. 237, 328–36.
Furness, J. B., Lleweliyn-Smith, I. J., Bornstein, J. C. &Costa, M. (1988) Chemical neuroanatomy and the analysis of neuronal circuitry in the enteric nervous system. InThe peripheral nervous system (edited byBjörklund, A., Hökfelt, T. &Owman, C.).Handbook of Chemical Neuroanatomy, Vol.6, pp. 161–218. Amsterdam, New York, Oxford: Elsevier.
Gábriel, R. &Eckert, M. (1989). Demonstration of GABA-like immunoreactivity in myenteric plexus of frog stomach.Histochemistry 91, 523–5.
Gábriel, R., &Halasy, K. &Csoknya, M. (1987) Cytochemical detection of NADH-diaphorase positive nerve cells in the intestinal canal of frog.Acta Biol. Szeged. 33, 85–95.
Gábriel, R., Halasy, K. &Csoknya, M. (1988) Visualization of neurons by NADH-diaphorase staining in the myenteric plexus of some invertebrate and vertebrate species.Z. mikr.-anat. Forsch. 102, 760–8.
Gábriel, R., Halasy, K., Fekete, É., Eckert, M. & Benedeczky, I. (1990) Distribution of GABA-like immunoreactivity in frog and chicken.Histochemistry, in press.
Gintzler, A. R. &Scalisi, I. A. (1982) Effects of opioids on non-cholinergic excitatory responses of the guinea-pig isolated ileum: inhibition of release of enteric substance P.Br. J. Pharmacol. 75, 199–205.
Gunn, M. (1951) A study of the enteric plexuses in some amphibians.Quart. J. Microsc. Sci. 92, 55–77.
Holzer, P. (1984) Characterization of the stimulus-induced release of immunoreactive substance P from the myenteric plexus of the guinea-pig small intestine.Brain Res. 287, 127–36.
Jensen, J., Holmgreen, S. &Jönsson, A.-C. (1987) Substance P-like immunoreactivity and the effects of tachkinins in the intestine of atlantic cod,Gadus morlua.J. Auton, Nerv. Syst.,20, 25–33.
Junquera, C., Azanza, M. J., Parra, P., Peg, M. T., Aisa, D., Romero, L. M. &Garin, P. (1988) Intrinsic and extrinsic innervation of the amphibians esophagic myenteric plexuses.Histol. Histopathol. 3, 115–24.
Katayama, Y. &North, R. A. (1978) Does substance P mediate slow synaptic excitation within the myenteric plexus?Nature 274, 387–8.
Katayama, Y., North, R. A. &Wileiams, J. T. (1979) The action of substance P on neurons of the myenteric plexus of the guinea-pig small intestine.Proc. R. Soc. B. 206, 191–208.
Monier, S. &Kitabgi, P. (1980) Substance P-induced autodesensitization inhibits atropin-resistant neurotensin-stimulated contractions in guinea-pig ileum.Eur. J. Pharmacol. 65, 461–2.
Niel, J. P., Bywater, R. A. R. &Taylor, G. S. (1983) Effect of substance P on non-cholinergic fast and slow poststimulus depolarization in the guinea-pig ileum.J. Auton. Nerv. Syst. 9, 573–84.
Nilsson, S. &Holmgren, S. (1989) Novel neurotransmitters in the autonomic nervous system of nonmammalian vertebrates.Pharmac. Ther. 41, 257–87.
North, R. A. (1982) Electrophysiology of the enteric nervous system.Neuroscience 7, 315–25.
Pearse, A. G. E. &Polak, J. (1975) Immunocytochemical localization of substance P in mammalian intestine.Histochemistry 41, 373–5.
Saffrey, M. J., Polak, J. M. &Burnstock, G. (1982) Distribution of vasoactive intestinal polypeptide-, substance P-, enkephalin- and neurotensin-like immunoreactive neurves in the chicken gut during development.Neuroscience 7, 279–93.
Smith, T. K., Furness, J. B., Costa, M. &Bronstein, J. C. (1988) An electrophysiological study of the projections of motor neurons that mediate non-cholinergic excitation in the circular muscle of the guinea-pig small intestine.J. Auton. Nerv. Syst. 22, 115–28.
Sundler, F., Hakanson, E., Larsson, L.-T., Brodin, E. &Nilsson, G. (1977) Substance P in the gut: an immunochemical and immunohistochemical study of its distribution and development. In Substance P (edited byVon Euler, S. S. &Pernow, B.), pp. 59–65. New York: Raven Press.
Tanaka, C., Taniyama, K. (1985) Substance P-provoked α-aminobutyric acid release from the myenteric plexus of the guinea-pig small intestine.J. Physiol. 362, 319–29.
Wood, J. D. (1984) Enteric neurophysiology.Am. J. Physiol. 247 (Gastrointest. Liver Physiol. 10), G585-G598.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gábriel, R. Distribution of substance p-like immunoreactivity in nerves of the gastrointestinal tract of the frogRana esculenta L. . Histochem J 22, 560–566 (1990). https://doi.org/10.1007/BF01005979
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01005979