Synopsis
A histochemical azo-coupling method for localizing folic acidin situ is described. Cat and rat liver, kidney, bone marrow and brain were found to be rich in folic acid; stomach, intestine, salivary glands, and blood contained less. Folic acid was localized in the cytoplasm of tissues having a very active metabolism, but in the nucleus of highly specialized cells such as neurons.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baugh, C. M. &Krumdieck, C. L. (1971). Naturally occurring folates.Ann. N. Y. Acad. Sci. 186, 7–28.
Blakley, T. L. (1969).The biochemistry of folic acid and related pteridines, pp. 8–57. Amsterdam, London: North-Holland.
Bratton, A. C. &Marshall, E. K. Jr. (1939). A new coupling compound for sulfanilamide determination.J. biol. Chem. 128, 537–40.
Bruemmer, N. Carver, M. J. &Thomas, L. E. (1957). A tryptophan histochemical method.J. Histochem. Cytochem. 5, 140–4.
Danielli, J. F. (1947). A study of techniques for the cytochemical demonstration of nuclei acids and some components of proteins.Symp. Soc. exp. Biol. 1, 101–13.
Diculescu, I., Onicescu &Mischiu, L. (1964). A histochemical analysis of dehydrogenase variety in the different types of muscular tissues.J. Histochem. Cytochem. 12, 145–52.
Diculescu, I. &Onicescu, D. (1965). A histochemical method for beta-hydroxyacyl CoA.Naturwiss. 52, 36–7.
Glenner, G. G. &Lillie, R. D. (1957). The histochemical demonstration of indole derivatives by the post-coupled-dimethyl-aminobenzyliden reaction.J. Histochem. Cytochem. 5, 279–96.
Grossowicz, N., Rachmilewitz, M. &Izak, G. (1963). Folate activity in rat tissue before and after pteroylglutamic acid load.Proc. Soc. exp. Biol. Med. 112, 486–9.
Grossowicz, N., Izak, G. &Rachmilewitz, M. (1964). Folate activity in tissues of rats on folate-free diets.Proc. Soc. exp. Biol. Med. 115, 953–7.
Hutchings, B. L., Stokstad, E. L. R., Boothe, J. H., Mowat, J. H., Waller, C. W., Angier, R. B., Semb, J. &Subbarow, Y. (1947). A chemical method for the determination of pteroylglutamic acid and related compounds.J. biol. Chem. 168, 705–8.
Hutchings, B. L., Stokstad, E. L. R., Mowat, J. H., Boothe, J. H., Waller, C. W., Angier, R. B., Semb, J. &Subbarow, Y. (1948). The isolation of the fermentation Lactobacillus casei factor.J. Am. chem. Soc. 70, 10–14.
Lillie, R. D. (1957). The xanthydrol reaction for pyrroles and indoles in histochemistry: zymogen granules, lens, enterochromaffin and melanins.J. Histochem. Cytochem. 5, 188–95.
Onicescu, D., Taşcă, L., &Trutza, E. (1970). A histochemical method for dihydrofolate reductase.Histochem. J. 2, 289–95.
Onicescu, D., Popescu, M., Taşcă, L. &Stoica, I. (1972). On the specificity of a histochemical method for localizing dihydrofolate reductase activity.Histochem. J. 4, 87–90.
Pearse, A. G. E. (1961). Proteins and amino-acids. In:Histochemistry, Theoretical and applied, pp. 98–9. London: Churchill.
Pearse, A. G. E. (1968). Proteins and amino-acids. In:Histochemistry. Theoretical and applied, Vol. 1. pp. 136–7, 165. London: Churchill.
Stoward, P. J. (1963). D. Phil. Thesis. University of Oxford.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Onicescu, D., Popescu, M. & Taşcå, L. Thein situ localization of folic acid. Histochem J 7, 427–434 (1975). https://doi.org/10.1007/BF01003880
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01003880