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Chemical Fixation, Immunofluorescence, and Immunogold Labeling of Electron Microscopical Sections

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Plant Cell Morphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1992))

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Abstract

Knowledge about the spatiotemporal distribution patterns of proteins and other molecules of the cell is essential for understanding their function. A widely used technique is immunolabeling which uses specific antibodies to reveal the distribution of molecular components at various structural levels. Immunofluorescence gives an overview about the distribution of molecules at the level of the fluorescence or confocal laser scanning microscope. Electron microscopy offers the highest resolution of morphological techniques and is thus an indispensable tool for the analysis of molecule distribution patterns at the subcellular level. In this chapter we describe selected routine methods for immunofluorescence and for labeling ultrathin sections of resin-embedded material with antibodies conjugated to colloidal gold, including protocols for chemical fixation, embedding, and sectioning.

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Acknowledgments

This work was supported by the Austrian Science Fund (project no. P 27536-B16 to IF).

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Authors and Affiliations

  1. Department of Biosciences, University of Salzburg, Salzburg, Austria

    Ilse Foissner & Margit Hoeftberger

Authors
  1. Ilse Foissner
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  2. Margit Hoeftberger
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Corresponding author

Correspondence to Ilse Foissner .

Editor information

Editors and Affiliations

  1. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Fatima Cvrčková

  2. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Viktor Žárský

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Foissner, I., Hoeftberger, M. (2019). Chemical Fixation, Immunofluorescence, and Immunogold Labeling of Electron Microscopical Sections. In: Cvrčková, F., Žárský, V. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1992. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9469-4_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9469-4_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9468-7

  • Online ISBN: 978-1-4939-9469-4

  • eBook Packages: Springer Protocols

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