Abstract
81 wild forms and 110 cultivated cowpea,Vigna unguiculata, accessions from 21 countries of Africa were screened for variability in seed storage proteins. Total seed proteins, albumin and globulin fractions were investigated by means of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and isoelectric focusing (IEF) of nonreduced and/or reduced samples in one- and two-dimensional procedures. The globulin fraction is heterogeneous in molecular weight and contains both legumin-like components and three to six nondisulfide-linked subunits. Three globulin subunits, with molecular weights 110, 76, and 41 kD were found to be composed of disulfide-linked polypeptides. In the nondisulfide-linked fraction, both cultivated and wild forms exhibited patterns of four types (A–D). This fraction contains polypeptide subunits of molecular weights 62, 56, and 52 kD for A type, 62, 56, 54, and 52 kD for B type, 62, 56, 52, and 50 kD for C type, and at least 62, 56, 54, 52, 50, and 49 kD for D type. These subunits present similar multiple charge forms but C and D types possess more basic specific 50 and 49 kD nondisulfide linked components. Major albumin fraction contains subunits of 94, 86, 32, and 24kD. No infraspecific variation was observed in albumin or legumin-like fractions. The discussion is focussed on the relations between genetic variability assessed by storage protein coding genes and phenotypic variability.
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Bhatty, R. S., 1982: Albumin proteins of eight edible grain legume species: electrophoretic patterns and amino acid composition. — J. Agric. Food Chem.30: 620–622.
Blagrove, R. J., Gillepsie, J. M., 1978: Variability of the seed globulins of winged beanPsophocarpus tetragonolobus (L.) DC. — Austral. J. Pl. Physiol.5: 371–375.
Bressani, R., 1985: Nutritive value of cowpea. — InSingh, S. R., Rachie, K. O., (Eds): Cowpea research, production, and utilization, pp. 353–359. — Chichester: Wiley.
Brown, J. W. S., Ma, Y., Bliss, F. A., Hall, T. C., 1981: Genetic variation in the subunit of globulin-1 storage protein of french bean. — Theor. Appl. Genet.59: 83–88.
Carasco, J. F., Croy, R., Derbyshire, E., Boulter, D., 1978: The isolation and characterization of the major polypeptides of the seed globulin of Cowpea [Vigna unguiculata (L.)Walp.] and their sequential synthesis in developing seeds. — J. Exper. Bot.29: 309–323.
Chevalier, A., 1944: La dolique de Chine en Afrique. — Rev. Bot. Appl. Agric. Trop.24: 128–152.
Dalgalarrondo, M., Raymond, J., Azanza, J. L., 1984: Sunflower seed proteins: characterization and subunit composition of the globulin fraction. — J. Exper. Bot.35: 1618–1628.
—, —, —, 1985: Sunflower seed protein: size and charge heterogeneity in subunits of the globulin fraction. — Biochimie67: 629–632.
Davies, C. S., Coates, J. B., Nielsen, N. C., 1985: Inheritance and biochemical analysis of four electrophoretic variants of beta-conglycinin from soybean. — Theor. Appl. Genet.71: 351–358.
Debouck, D. G., Maquet, A., Posso, C. E., 1989: Biochemical evidence for two different gene pool in lima beans,Phaseolus lunatus L. — Annu. Rept. Bean Improvement Coop.32: 58–59.
Derbyshire, E., Wright, D. J., Boulter, D., 1976: Legumin and vicilin, storage proteins of legume seeds. — Phytochemistry15: 3–24.
Derchef-Hamey, S., Mimouni, B., Raymond, J., Azanza, J. L., 1990: Partial characterization of polypeptide components of sunflower (Helianthus annuus L.) seed albumin fraction. — Die Nahrung34: 387–398.
Evans, I. M., Boulter, D., 1974: Chemical methods suitable for screening for protein content and quality in cowpea (Vigna unguiculata) meals. — J. Sci. Food Agric.30: 948–958.
Gepts, P., Bliss, F. A., 1985: F1 hybrids weakness in the common bean. — J. Heredity76: 447–450.
—, —, 1986: Phaseolin variability among wild and cultivated common beans (Phaseolus vulgaris) from Columbia. — Econ. Bot.40: 469–478.
—, 1986: Phaseolin-protein variability in wild forms and landraces of the common bean (Phaseolus vulgaris): evidence for multiple center of domestication. — Econ. Bot.40: 451–468.
—, 1988: Dissemination pathways of common bean (Phaseolus vulgaris, Fabaceae) deduced from Phaseolin electrophoretic variability. The Americas. — Econ. Bot.42: 73–85.
Hernandez, I. M., Truong, V. D., Mendoza, E. M. T., 1983: Characterization of seed proteins ofVigna unguiculata (L.)Walp. Kalikasan, Philip. — J. Biol.12: 115–126.
Hymowitz, T., Kaisuma, N., 1979: Dissemination of soybeans (Glycine max.): seed protein electrophoresis profiles among japanese cultivars. — Econ. Bot.33: 311–319.
—, —, 1981: Soybean seed protein electrophoresis profiles from 15 Asian countries or regions: hypothesis on paths of dissemination of soybeans from China. — Econ. Bot.35: 10–23.
Khan, M. R. I., Gatehouse, J. A., Boulter, D., 1980: The seed proteins of cowpea [Vigna unguiculata (L.)Walp.]. — J. Exper. Bot.31: 1599–1611.
Kitamura, K., Davies, C. S., Nielsen, N. C., 1984: Inheritance of alleles for CGY 1 and Gy 4 storage protein genes in soybean. — Theor. Appl. Genet.68: 253–257.
Kortt, A., 1983: Comparative studies on the storage proteins and anti-nutritional factors from seeds ofPsophocarpus tetragonolobus (L.) DC. from five south-east asian countries. — Qual. Pl. Foods Human Nutr.33: 29–40.
Krishna, T. G., Mitra, R., 1988: The probable genome donors toArachis hypogea L. based on arachin seed storage protein. — Euphytica37: 47–52.
Krochko, J. E., Bowley, S. R., Bewley, J. D., 1990: A comparison of seed storage proteins in subspecies and cultivars ofMedicago sativa. — Canad. J. Bot.68: 940–948.
Ladizinsky, G., Hymowitz, T., 1979: Seed protein electrophoresis in taxonomic and evolutionary studies. — Theor. Appl. Genet.54: 145–151.
—, 1980: Seed protein profiles of pigeon pea (Cajanus cajan) and someAtylosia species. — Euphytica29: 313–317.
Laemmli, U. K., 1970: Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. — Nature227: 681–685.
Loveless, M. D., Hamrick, J. L., 1984: Ecological determinants of genetic structures in plant populations. — Annu. Rev. Ecol. Syst.15: 65–95.
Maquet, A., Gutierrez, A., Debouck, D. G., 1990: Further evidence for the existence of two gene pools in lima beans. — Annu. Rept. Bean Improvement Coop.33: 128–129.
Mohamed, M. A., Lerro, K. A., Prestwich, G. D., 1989: Polyacrylamide gel miniaturization improves protein visualization and autoradiographic detection. — Anal. Biochem.177: 187–290.
Murray, D. R., Mackenzie, K. F., Vairinhos, F., Peoples, M. B., Atkins, C. A., 1983: Electrophoresis studies of the seed proteins of cowpea [Vigna unguiculata (L.)Walp.]. — Z. Pflanzenphysiol.109: 363–370.
Ologhoho, A. D., Fetuga, B. L., 1982: Chemical composition of promising cowpea (Vigna unguiculata) varieties. — Nutr. Rep. Intern.25: 913–916.
Paino D'Urzo, M., Pedalino, M., Grillo, S., Rao, R., Tucci, M., 1990: Variability in major seed proteins in differentVigna species. — InNg, N. Q., Monti, L. M., (Eds): Cowpea genetic resources, pp. 90–100. — Ibadan: IITA.
Pasqualini, S., Lluch, C., Antonielli, M., 1991: Seed storage proteins in several genetic lines ofVicia faba. — Pl. Physiol. Biochem.29: 507–515.
Pasquet, R. S., 1992: Classification infraspécifique des formes spontanées deVigna unguiculata (L.)Walp. (Fabaceae) à partir de données morphologiques. — Bull. Jard. Bot. Nat. Belg.62: 127–173.
—, 1993: Variation at isoenzyme loci in wildVigna unguiculata (L.)Walp. (Fabaceae, Phaseoleae). — Pl. Syst. Evol.186: 157–173.
Pedalino, M., Paino D'Urzo, M., Costa, A., Grillo, S., Rao, R., 1990: Biochemical characterization of cowpea seed proteins. — InNg, N. Q., Monti, L. M., (Eds): Cowpea genetic resources, pp. 81–89. — Ibadan: IITA.
Raymond, J., Inquello, V., Azanza, J. L., 1991: The seed proteins of sunflower: comparative studies of cultivars. — Phytochemistry30: 2849–2856.
Romero-Andreas, J., Bliss, F. A., 1985: Heritable variation in the phaseolin protein of non-domesticated common bean,Phaseolus vulgaris. — Theor. Appl. Genet.71: 478–480.
Schinkel, C., Gepts, P., 1988: Phaseolin diversity in the tepary bean,Phaseolus acutifolius A. Gray. — Pl. Breed.101: 292–301.
Schmit, V., Debouck, D. G., 1991: Observations on the origin ofPhaseolus polyanthus Greenman. — Econ. Bot.45: 345–364.
Sefah-Debeh, S., Stanley, D., 1979: Cowpea proteins. Characterization of water extractable proteins. — J. Agr. Food Chem.27: 1244–1247.
Tomooka, N., Lairungreang, C., Nakeeraks, P., Egawa, Y., Thavarasook, C., 1992: Center of genetic diversity and dissemination pathways in mung bean deduced from seed protein electrophoresis. — Theor. Appl. Genet.83: 289–293.
Towbin, H., Staehelin, T., Gordon, J., 1979: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. — Proc. Natl. Acad. Sci. USA76: 4350–4354.
Tsukada, Y., Kitamura, K., Harada, K., Kaizuma, N., 1986: Genetic analysis of subunits of two major storage proteins (beta-conglycinin) of soybean seeds. — Japan J. Pl. Breed.36: 390–400.
Tucci, M., Capparelli, R., Costa, A., Rao, R., 1991: Molecular heterogeneity and genetics ofVicia faba seed storage proteins. — Theor. Appl. Genet.81: 50–58.
Vidovic, M., Murray, D. R., 1984: Mobilization of nitrogen during imbibition and germination of seeds ofPhaseolus vulgaris andVigna unguiculata. — Z. Pflanzenphysiol.113: 117–128.
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Fotso, M., Azanza, JL., Pasquet, R. et al. Molecular heterogeneity of Cowpea (Vigna unguiculata Fabaceae) seed storage proteins. Pl Syst Evol 191, 39–56 (1994). https://doi.org/10.1007/BF00985341
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DOI: https://doi.org/10.1007/BF00985341