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Supra-additive activation of guinea-pig superior cervical ganglion adenylate cyclase by PGE2 andd-Ala2-Met-enkephalinamide: Role of GTP

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Abstract

The effects of guanine nucleotides were tested on basal and agonist-modulated adenylate cyclase in guinea-pig superior cervical ganglion crude membrane preparations. GTPγS and Gpp(NH)p dose-dependently stimulate, while GDPβS inhibits, both the basal and the prostaglandin E2-stimulated enzyme activity. Low GTP doses, up to 10−5M, stimulate, while higher doses inhibit, the ganglionic adenylate cyclase. The GTP-induced diphasic pattern is maintained also in the presence of prostaglandin E2,d-Ala2-Met-enkephalinamide, or a combination of the two drugs. However, the opioid inhibits the enzyme activity, but only at high GTP doses, while the prostaglandin stimulates the enzyme at all GTP concentrations. The effect is potentiated by a combination of prostaglandin and enkephalin. The enhancing effect of the prostaglandin and of the combination with enkephalin is maximally expressed at high, almost physiological, GTP doses.

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Authors and Affiliations

  1. Institute of General Physiology, University of Ferrara, Via L. Borsari n. 46, 44100, Ferrara, Italy

    Carla Biondi, Pier Giorgio Borasio, Maria Enrica Ferretti & Maria Cristina Pareschi

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  1. Carla Biondi
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  2. Pier Giorgio Borasio
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  3. Maria Enrica Ferretti
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  4. Maria Cristina Pareschi
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Biondi, C., Borasio, P.G., Ferretti, M.E. et al. Supra-additive activation of guinea-pig superior cervical ganglion adenylate cyclase by PGE2 andd-Ala2-Met-enkephalinamide: Role of GTP. Neurochem Res 15, 785–789 (1990). https://doi.org/10.1007/BF00968555

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  • DOI: https://doi.org/10.1007/BF00968555

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