Abstract
A rapid and specific method for assaying 4-aminobutyrate-2-oxoglutarate aminotransferase was developed. The method was based on the selectivity of ion exchange resin and the speed of vacuum filtration. With this new method, the aminotransferase activity in various tissues has been determined as follows: brain, 10.2; spinal cord, 11.8; liver, 5.7; kidney, 4.6; heart, 0.5; lung, 0.4 nmol glutamate formed/min/mg. No activity could be detected in muscle preparations. When the aminotransferases were tested with the antibody, against the purified 4-aminobutyrate aminotransferase from brain, no difference could be detected among brain, spinal cord, and kidney preparations as judged from the results of immunodiffusion, inhibition of enzyme activity by antibody, and microcomplement fixation. It is concluded that 4-aminobutyrate aminotransferases from various tissues of the mouse are probably identical or closely related.
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This work was supported in part by Grant No. NS 13224 and P01 NS 12116 from the National Institutes of Health, U.S.A. and Grant from Huntington's Chorea Foundation.
All correspondence should be addressed to Dr. Wu.
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Wu, JY., Moss, L.G. & Chude, O. Distribution and tissue specificity of 4-aminobutyrate-2-oxoglutarate aminotransferase. Neurochem Res 3, 207–219 (1978). https://doi.org/10.1007/BF00964061
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DOI: https://doi.org/10.1007/BF00964061