Abstract
Nine Swedish populations, 1–5 individuals/population, and one cultivated individual of the rare speciesVicia pisiformis were investigated for genetic variation. In hybridizations with two rDNA probes using 8 restriction enzymes, only two individuals belonging to one population were polymorphic. A map of the rDNA gene cluster was constructed for four of the restriction enzymes used. Two of the polymorphic sites were mapped and were found to be located outside regions coding for rRNA, presumably caused by single point mutations or small deletions. The repeat length of the rDNA region was c. 10,000 bp, which corresponds well with the size found for other species belonging toFabaceae. No length polymorphism was found in the intergenic spacer, contrary to the situation found for most other plant species investigated for rDNA variation. The haplotype diversity for the species (Hsp Shannon) was very low (0.055). Within-population values (Hpop) was 0 for all populations except the variable one, which had 0.301. PCR amplification with 6 random primers also revealed very low levels of genetic diversity. A polymorphism was observed in a limited number of individuals for four populations. Hsp was 0.065 and\(\bar Hpop\) was 0.050. The average D value (Wetton) for the PCR haplotypes was 0.99.
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Arnold, M. L., Bennett, B. D., Zimmer, E. A., 1990: Natural hybridization betweenIris fulva andIris hexagona: pattern of ribosomal DNA variation. — Evolution44: 1512–1521.
Brauner, S., Crawford, D. J., Stuessy, T. F., 1992: Ribosomal DNA and RAPD variation in the rare plant familyLactoridaceae. — Amer. J. Bot.79: 1436–1439.
Capesius, I., 1991: Nucleotide sequence of an rRNA gene fromSinapis alba. — Pl. Mol. Biol.16: 1093–1094.
Capossela, A., Silander, J. A. Jr., Jansen, R. K., Bergen, B., Talbot, D. R., 1992: Nuclear ribosomal DNA variation among ramets and genets of white clover. — Evolution46: 1240–1247.
Chalmers, K. J., Waugh, R., Sprent, J. I., Simons, A. J., Powell, W., 1992: Detection of genetic variation between and within populations ofGliricidia sepium andG. maculata using RAPD markers. — Heredity69: 465–472.
Databanken för hotade arter, naturvårdsverket (Threatened Species Unit, Swedish Environmental Protection Agency), 1991: Hotade växter i Sverige 1990. (Sweden's red list on vascular plants, bryophytes, lichens, and macrofungi). — Lund: Svensk Bot. Tidskr. In Swedish with English abstract.
Demeke, T., Adams, R. P., Chibbar, R., 1992: Potential taxonomic use of random amplified DNA (RAPD) — a case study inBrassica. — Theor. Appl. Genet.84: 990–994.
Doyle, J. J., Doyle, J. J., 1987: A rapid DNA isolation procedure for small amounts of fresh leaf tissue. — Phytochem. Bull.19: 11–15.
, 1985: An intergenic hybrid in theSaxifragaceae: evidence from ribosomal RNA genes. — Amer. J. Bot.72: 1388–1391.
Gustafsson, L., 1991: Ärtvicker,Vicia pisiformis, i Sverige. (Vicia pisiformis in Sweden). — Svensk Bot. Tidskr.85: 21–32. In Swedish with English abstract.
Hamrick, J. L., Godt, M. J. W., 1990: Allozyme diversity in plant species. — InBrown, A. H. D., Clegg, M. T., Kahler, A. L., Weir, B. S., (Eds): Plant population genetics, breeding and genetic resources, pp. 43–63. — Sunderland, Mass.: Sinauer.
Hanelt, P., Mettin, D., 1989: Biosystematics of the genusVicia L. (Leguminosae). — Annu. Rev. Ecol. Syst.20: 199–223.
Hu, J., Quiros, C. F., 1991: Identification of broccoli and cauliflower with RAPD markers. — Pl. Cell Reports10: 505–511.
Hultén, E., Fries, M., 1986: Atlas of North European vascular plants north of the Tropic of Cancer. II. — Königstein: Koeltz.
Jorgensen, R. A., Cuellar, R. E., Thompson, W. F., Kavanagh, T. A., 1987: Structure and variation in ribosomal RNA genes of pea. — Pl. Mol. Biol.8: 3–12.
Kato, A., Nakajima, T., Yamashita, J., Yakura, K., Tanifuji, S., 1990: The structure of the large spacer region of the rDNA inVicia faba andPisum sativum. — Pl. Mol. Biol.14: 983–993.
King, L. M., Schaal, B. A., 1989: Ribosomal-DNA variation and distribution inRudbeckia missouriensis. — Evolution43: 1117–1119.
Kiss, T., Szkukálek, A., Solymosy, F., 1989: Nucleotide sequence of a 17 S (18 S) rRNA gene from tomato. — Nucleic Acids Res.17: 2127.
Learn, G. H., Schaal, B. A., 1987: Population subdivision for ribosomal DNA repeat variants inClematis fremontii. — Evolution41: 433–438.
Lesica, P., Leary, R. F., Allendorf, F. W., Bilderback, D. E., 1988: Lack of genic diversity within and among populations of an endangered plant,Howellia aquatilis. — Conserv. Biol.2: 275–282.
Lewontin, R. C., 1972: The apportionment of human diversity. — Evol. Biol.6: 381–398.
Nybom, H., Schaal, B. A., 1990: DNA “fingerprints” reveal genotypic distributions in natural populations of blackberries and raspberries (Rubus, Rosaceae). — Amer. J. Bot.77: 883–888.
, 1990: Genetic variation detected by use of the M 13 “DNA fingerprint” probe inMalus, Prunus, andRubus (Rosaceae). — Theor. Appl. Genet.79: 153–156.
Rathgeber, J., Cepesius, I., 1989: Nucleotide sequence of the 18 S–25 S spacer region from mustard DNA. — Nucleic Acids Res.17: 7522.
Rieseberg, L. H., Soltis, D. E., Palmer, J. E., 1988: A molecular reexamination of introgression betweenHelianthus annuus andH. bolanderi (Compositae). — Evolution42: 227–238.
Rogers, S. O., Bendich, A. J., 1986: Variation in the ribosomal RNA genes among individuals ofVicia faba. — Pl. Mol. Biol.6: 339–345.
, 1987a: Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. — Pl. Mol. Biol.9: 509–520.
, 1987b: Heritability and variability in ribosomal RNA genes ofVicia faba. — Genetics117: 285–295.
Saghai-Maroof, M. A., Soliman, K. M., Jorgensen, R. A., Allard, R. W., 1984: Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. — Proc. Natl. Acad. Sci. USA81: 8014–8018.
, 1990: Genetic diversity and ecogeographical differentiation among ribosomal alleles in wild and cultivated barley. — Proc. Natl. Acad. Sci. USA87: 8486–8490.
Schaal, B. A., Leverich, W. J., Nieto-Sotelo, J., 1987: Ribosomal DNA variation in the native plantPhlox divaricata. — Mol. Biol. Evol.4: 611–621.
, 1988: Ribosomal DNA variation within and among plant populations. — Ann. Missouri Bot. Gard.75: 1207–1216.
, 1991: DNA variation in plant populations. — Trends Ecol. Evol.6: 329–333.
Simon, J. P., Bergeron, Y., Gagnon, D., 1986: Isozyme uniformity in populations of red pine (Pinus resinosa) in the Abitibi Region, Quebec. — Canad. J. For. Res.16: 1133–1135.
Smith, R. L., Sytsma, K. J., 1990: Evolution ofPopulus nigra (sect.Aigeiros): Introgressive hybridization and the chloroplast contribution ofPopulus alba (sect.Populus). — Amer. J. Bot.77: 1176–1187.
Unfried, I., Gruendler, P., 1990: Nucleotide sequence of the 5.8 S and 25 S rRNA genes and of the internal transcribed spacers fromArabidopsis thaliana. — Nucleic Acids Res.18: 4011.
, 1989: Nucleotide sequence of the 18 S rRNA gene fromArabidopsis thaliana Co 10. — Nucleic Acids Res.17: 7513.
Vierling, R. A., Nguyen, H. T., 1992: Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes. — Theor. Appl. Genet.84: 835–838.
Waller, D. M., O'Malley, D. M., Gawler, S. C., 1987: Genetic variation in the extreme endemicPedicularis furbishiae (Scrophulariaceae). — Conserv. Biol.1: 335–340.
Welsh, J., McClelland, M., 1990: Fingerprinting genomes using PCR with arbitrary primers. — Nucleic Acids Res.18: 7213–7218.
Wetton, J. H., Carter, R. E., Parkin, D. T., Walters, D., 1987: Demographic study of a wild house sparrow population by DNA fingerprinting. — Nature327: 147–149.
Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., Tingey, S. V., 1990: DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. — Nucleic Acids Res.18: 6531–6535.
Wilson, E. O., 1988: The current state of biodiversity. — InWilson, E. O., (Ed.): Biodiversity, pp. 3–18. — Washington D.C.: National Academy Press.
Yakura, K., Tanifuji, S., 1983: Molecular cloning and restriction analysis inVicia faba. — Pl. Cell Physiol.22: 1105–1111.
, 1984: Length heterogeneity of the large spacer ofVicia faba rDNA is due to the differing number of a 325 bp repetitive sequence element. — Mol. Gen. Genet.193: 400–405.
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Gustafsson, L., Gustafsson, P. Low genetic variation in Swedish populations of the rare speciesVicia pisiformis (Fabaceae) revealed with rflp (rDNA) and RAPD. Pl Syst Evol 189, 133–148 (1994). https://doi.org/10.1007/BF00939722
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DOI: https://doi.org/10.1007/BF00939722