Skip to main content
SpringerLink
Log in

Alteration of polymorphonuclear leukocyte surface charge by endogenous and exogenous chemotactic factors

  • Original Articles
  • Published:
Inflammation Aims and scope Submit manuscript

Abstract

We investigated the effects of chemotaxins on the surface charge of isolated human PMN. Chemotaxis was ascertained using Boyden chambers. Surface charge was calculated using data derived from cell mobility as measured in a cytophorometer. The electrophoretic mobility of cells exposed to all chemotactic agents studied was altered. Endotoxin-activated serum containing C5a, PMN lysosomal extracts from “resting” and from crystal-stimulated cells, and Gly-His-Gly, a synthetic tripeptide, all significantly reduced the net negative surface charge of isolated neutrophils. Only chemotactically active substances effected this change; controls including heat-inactivated serum, other subcellular fractions, and an inert tripeptide, Gly-Gly-Gly, did not alter cell mobility in an electric field. These findings are consistent with studies by others on the effects of chemotactic factors on cation fluxes and cell aggregation and suggest a possible mechanism by which PMN directional migration is regulated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Harris, H. 1954. Role of chemotaxis in inflammation.Physiol. Rev. 34:529.

    Google Scholar 

  2. Ward, P. A. 1974. Leukotaxis and leukotactic disorders.Am. J. Pathol. 77:520.

    Google Scholar 

  3. Gallin, J. I., andS. M. Wolff. 1975. Leucocyte chemotaxis: Physiological considerations and abnormalities.Clin. Haematol. 4:567.

    Google Scholar 

  4. Zigmond, S. H. 1978. Chemotaxis by polymorphonuclear leukocytes.J. Cell Biol. 77:269.

    Google Scholar 

  5. Gallin, J. I., J. R. Durocher andA. P. Kaplan. 1975. Interaction of leukocyte chemotactic factors with the cell surface.J. Clin. Invest. 55:967.

    Google Scholar 

  6. O'flaherty, J. T., D. L. Kreutzer, andP. A. Ward. 1978. The influence of chemotactic factors on neutrophil adhesiveness.Inflammation 3:37.

    Google Scholar 

  7. Gallin, J. L., andA. S. Rosenthal. 1974. The regulatory role of divalent cations in human granulocyte chemotaxis.J. Cell Biol. 62:594.

    Google Scholar 

  8. Showell, H. J., andE. L. Becker. 1976. The effects of external K+ and Na+ on the chemotaxis of rabbit peritoneal neutrophils.J. Immunol 116:99.

    Google Scholar 

  9. Borel, J. F., H. U. Keller, andE. Sorkin. 1969. Studies on chemotaxis. XI. Effect on neutrophils of lysosomal and other subcellular fractions from leukocytes.Int. Arch. Allergy Appl. Immunol. 35:194.

    Google Scholar 

  10. Zigmond, S. H., andJ. G. Hirsch. 1973. Leukocyte locomotion and chemotaxis.J. Exp. Med. 137:387.

    Google Scholar 

  11. Phelps, P. 1969. Polymorphonuclear leukocyte mobility in vitro. III. Possible release of a chemotactic substance after phagocytosis of urate crystals by polymorphonuclear leukocytes.Arthritis Rheum. 12:197.

    Google Scholar 

  12. Spilberg, I., B. Mandell, andR. D. Wochner. 1974. Studies on crystal-induced chemotactic factor. I. Requirement for protein synthesis and neutral protease activity.J. Lab. Clin. Med. 83:56.

    Google Scholar 

  13. Spilberg, I., A. Gallacher, J. M. Mehta, andB. Mandell, 1976. Urate crystal-induced chemotactic factor.J. Clin. Invest. 58:815.

    Google Scholar 

  14. Showell, H. J., R. J. Freer, S. H. Zigmond, E. Schiffmann, S. Aswanikumar, B. Corcoran, andE. L. Becker. 1976. The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal enzyme secretion for neutrophils.J. Exp. Med. 143:1154.

    Google Scholar 

  15. Spilberg, I., B. Mandell, J. Mehta, T. Sullivan, andL. Simchowitz. 1978. Dissociation of the neutrophil functions of exocytosis and chemotaxis.J. Lab. Clin. Med. 92:297.

    Google Scholar 

  16. Takeuchi, A., R. H. Persellin. 1979. Cellular augmentation of polymorphonuclear leukocyte chemotaxis.Am. J. Physiol. 236:C22.

    Google Scholar 

  17. Altman, P. L., andD. D. Katz, editors. 1976. Cell Biology. Federation of American Societies for Experimental Biology, Bethesda, Maryland. 104–110.

    Google Scholar 

  18. Lowry, O. H., N. J. Rosebrough, A. L. Farr, andR. J. Randall. 1951. Protein measurement with the folin phenol reagent.J. Biol. Chem. 193:365.

    Google Scholar 

  19. Gallin, E. K., andJ. I. Gallin. 1977. Interaction of chemotactic factors with human macrophages.J. Cell Biol. 75:277.

    Google Scholar 

  20. Northrop, J. H. 1922. The stability of bacterial suspensions.J. Gen. Physiol. 4:629.

    Google Scholar 

  21. Weissmann, G. 1967. Structure and function of lysosomes.In Rheumatology-An Annual Review, J. Rotstein, editor. S. Karger, New York. 1–28.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Rheumatology, Department of Medicine, University of Texas Health Science Center at San Antonio, USA

    Terry M. Schaack, Akiteru Takeuchi, Isaias Spilberg & Robert H. Persellin

  2. Department of Medicine, Washington University School of Medicine, USA

    Terry M. Schaack, Akiteru Takeuchi, Isaias Spilberg & Robert H. Persellin

  3. John Cochran Veterans Administration Hospital, St. Louis, Missouri

    Terry M. Schaack, Akiteru Takeuchi, Isaias Spilberg & Robert H. Persellin

Authors
  1. Terry M. Schaack
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akiteru Takeuchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Isaias Spilberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robert H. Persellin
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schaack, T.M., Takeuchi, A., Spilberg, I. et al. Alteration of polymorphonuclear leukocyte surface charge by endogenous and exogenous chemotactic factors. Inflammation 4, 37–44 (1980). https://doi.org/10.1007/BF00914101

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00914101

Keywords

Search

Navigation