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Recombinant human granulocytemacrophage colony-stimulating factor induces granule exocytosis from human polymorphonuclear neutrophils

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Abstract

Exposure of human polymorphonuclear neutrophils (PMNs) to recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) resulted in a time- and concentration-dependent (3–100 units/ml) extracellular release of a specific (vitamin B12-binding protein) but not azurophil granule constituent (myeloperoxidase). Negligible granule exocytosis occurred if PMNs were not preincubated with cytochalasin B prior to contact with GM-CSF. The extent of degranulation elicited with GM-CSF was reduced but not abolished when PMNs were incubated with EGTA in calcium-free medium. GM-CSF did not stimulate a rise in the cytosolic-free calcium concentration ([Ca2+],), and it had no effect on PMN protein kinase C (PKC) activity.

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  1. Department of Hypersensitivity Diseases Research, The Upjohn Company, 49008, Kalamazoo, Michigan

    Robert J. Smith, James M. Justen & Laurel M. Sam

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Smith, R.J., Justen, J.M. & Sam, L.M. Recombinant human granulocytemacrophage colony-stimulating factor induces granule exocytosis from human polymorphonuclear neutrophils. Inflammation 14, 83–92 (1990). https://doi.org/10.1007/BF00914032

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