Advertisement

Inflammation

, Volume 6, Issue 1, pp 1–11 | Cite as

Effects of supernatants of polymorphonuclear neutrophils recruited by different inflammatory substances on mitogen responses of lymphocytes

  • Mary L. Rodrick
  • Ira B. Lamster
  • Stephen T. Sonis
  • Suzanne G. Pender
  • Amy B. Kolodkin
  • John E. Fitzgerald
  • Richard E. Wilson
Original Articles

Abstract

Two different substances, glycogen and thioglycollate, were used to recruit early peritoneal exudate cells (4 h). In the acute phase of the inflammatory response the cellular infiltrate is large, and the predominant cell (>95%) is the polymorphonuclear neutrophil. Supernatant had differing effects on lymphocyte responses to the mitogens PHA and LPS, also carried out in serum-free media, depending on recruiting substance and time of culture. While glycogen-recruited PMN supernatant (GPMN-S) always enhanced splenocyte responses to PHA, thioglycollate-recruited cells (TPMN-S) did not produce an enhancing factor until the cells had been in culture for 24 h. Whereas GPMN-S enhanced the splenocyte response to LPS only after 1 or 4 h of culture, TPMN-S failed to have any significant effect. Thymocyte responses to PHA were facilitated by all supernatants. Dilution of the soluble PMN factors resulted in a suppressive effect on splenocyte responses to both PHA and LPS, regardless of whether PMN were recruited by the thioglycollate or glycogen or of the time of cell incubation. These results indicate that PMN-rich cell populations of different types of activity are recruited by glycogen and thioglycollate and that these cells produce factors capable of potentiating, enhancing, or suppressing responses to T- or B-cell mitogens by normal syngeneic lymphocytes.

Keywords

Inflammatory Response Cell Population Acute Phase Suppressive Effect Cell Incubation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Yoshinaga, M., S. Nakamura, andH. Hayashi. 1975. Interaction between lymphocytes and inflammatory exudate cells. I. Enhancement of thymocyte response to PHA by product(s) of polymorphonuclear leukocytes and macrophages.J. Immunol. 115:533.Google Scholar
  2. 2.
    Yoshinaga, M., K. Nishime, S. Nakamura, andF. Goto. 1980. APMN-derived factor that enhanced DNA-systhesis in PHA or antigen-stimulated lymphocytes.J. Immunol. 124:94.Google Scholar
  3. 3.
    Hsu, C., Y. B. Wu, andJ. Rivera-Arcilla. 1979. Inhibition of lymphocyte reactivity in vitro by autologous polymorphonuclear cells (PMN).Cell. Immunol. 48:375.Google Scholar
  4. 4.
    Pastore, J., andR. Panush. 1977. Modulation of human lymphocyte responses by neutrophil-derived substances.Clin. Res. 25:24A.Google Scholar
  5. 5.
    Vischer, T., U. Bretz, andM. Baggiolini. 1976. In vitro stimulation of lymphocytes by neutral proteases from human polymorphonuclear leukocyte granules.J. Exp. Med. 144:863.Google Scholar
  6. 6.
    Nakamura, S., M. Yoshinaga, andH. Hayashi. 1976. Interaction between lymphocytes and inflammatory exudate cells. II. A proteolytic enzyme released by PMN as a possible mediator of enhancement of thymocyte response.J. Immunol. 117:1.Google Scholar
  7. 7.
    Tchorzewski, H., Z. Sulowska, andA. Denys. 1973. A new lymphocyte transforming factor derived from the lysosomes from polymorphonuclear leukocytes.Experientia 29:481.Google Scholar
  8. 8.
    Tchorzewski, H., andZ. Sulowska. 1980. The mitogenic and enzymatic activities of polymorphonuclear leucocyte (PMNL) lysosomal proteins examined in vitro.Arch. Immunol. Ther. Exp. 28:1.Google Scholar
  9. 9.
    Lamster, I., S. Sonis, D. Mirando, andR. Wilson. 1979. Influence of supernatant from polymorphonuclear leukocytes on blastogenesis of syngeneic and allogeneic murine splenocytes.Clin. Exp. Immunol,36:285.Google Scholar
  10. 10.
    Lamster, I., S. Sonis, D. Mirando, A. Kolodkin, M. Rodrick, andR. Wilson. 1980. Modification of in vitro and in vivo immune function by acute inflammatory cells.Transplantation 30:244.Google Scholar
  11. 11.
    Rodrick, M., I. Lamster, S. Sonis, andR. Wilson. 1980. Effect of neutrophils on antibody synthesis in mice.J. Dent. Res. 19:460.Google Scholar
  12. 12.
    Coutinho, A., E. Gronowicz, G. Moller, andH. Lemke. 1976. Polyclonal B cell activators.In Mitogens in Immunobiology, Polyclonal B-Cell Activators (PBA).J. Oppenheim andD. Rosenstreich, eds. Academic Press, New York, 173–190.Google Scholar
  13. 13.
    Henson, R. B. Zanolari, N. Schwartzman, andS. Hong. 1978. Intracellular control of human neutrophil secretion. I. C5a-induced stimulus-specific desensitization and the effects of cytochalasain B.J. Immunol. 121:851.Google Scholar
  14. 14.
    English, L. S. 1978. Immune response regulation by lymphocyte products. Potentiation and suppressor factors detected during responses to concanavalin-A.E. coli lipopolysaccharide and dinitrophenylated bovine serum albumin.Cell. Immunol. 37:349.Google Scholar
  15. 15.
    Tchorzewski, H., J. Czernicki, Z. Sulowska, B. Rzetelski, andJ. Pacezesniak. 1980. Subcellular distribution of lymphocyte-stimulating factors in human granulocytes.Immunol. Lett. 1:311.Google Scholar
  16. 16.
    Broxmeyer, H., P. Ralph, J. Bognacki, P. Kincade, andM. Desousa. 1980. A subpopulation of human polymorphonuclear neutrophils contain an active form of lactoferrin capable of binding to human monocytes and inhibiting production of granulocytemacrophage colony stimulatory activities.J. Immunol. 125:903.Google Scholar
  17. 17.
    Nathan, C, H. Murray, andZ. Cohn. 1980. Current concepts: The macrophage as an effector cell.N. Engt. J. Med. 303:622.Google Scholar
  18. 18.
    Klebanoff, S., andR. Clark, eds. 1978. The Neutrophil: Function and Clinical Disorders. North-Holland Publishing Co., New York.Google Scholar
  19. 19.
    Knop, J. 1980. Influence of various macrophage populations in Con-A-induced T-cell proliferation.Immunology 41:379.Google Scholar
  20. 20.
    Okuda, K., K. Toni, Y. Ishigatosubo, S. Yokota, andC. David. 1980. Antigen-pulsed neutrophils bearing Ia antigens can induce T lymphocyte proliferative response to the syngeneic or semisyngeneic antigen-primed T lymphocytes.Transplantation 30:368.Google Scholar
  21. 21.
    Aarden, L. T., J. C. Brunner, J. M. Cerottini, A. Dayer, C. De Weck, G. Dinarello, J. Di Sabato, I. Farrar, S. Gery, S. Gillis, R. Handschumacher, C. Henney, M. Hoffman, W. Koopman, S. Krane, L. Lachman, I. Lifkowits, R. Mishell, S. Mizel, J. Oppenheim, V. Paetkau, J. Plate, M. Rollinghoff, D. Rosenstreich, A. Rosenthal, L. Rosenwasser, A. Shimpl, H. Shin, P. Simon, K. Smith, H. Wagner, J. Watson, E. Wecker, andD. Wood. 1979. Revised nomenclature for antigen-nonspecific T-cell proliferation and helper factorsJ. Immunol. 123:2928.Google Scholar

Copyright information

© Plenum Publishing Corporation 1982

Authors and Affiliations

  • Mary L. Rodrick
    • 1
  • Ira B. Lamster
    • 1
  • Stephen T. Sonis
    • 1
  • Suzanne G. Pender
    • 1
  • Amy B. Kolodkin
    • 1
  • John E. Fitzgerald
    • 1
  • Richard E. Wilson
    • 1
  1. 1.Department of SurgeryPeter Bent Brigham Hospital and Harvard Medical SchoolBoston

Personalised recommendations