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Dynamics of amino acid composition of the medium during culture of isolated liver and kidneys by the controlled perfusion method

  • Biochemistry and Biophysics
  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

Abstract

The dynamics of the amino acid composition of the medium was investigated during perfusion of the dog liver and kidney for 6 h with a mixture of autogenous plasma and medium No. 199 in the ratio of 2∶3. During culture of the kidney for 6 h the histidine concentration in the medium increased by 2.2 times compared with initially, the concentration of glutamic acid by 1.7 times, and of alanine and lysine by 1.6 times, whereas the concentrations of arginine, serine, and aspartic acid fell by 3.3 times and those of glutamine with threonine by 2.5 times. During perfusion of the liver the concentration of glutamic acid rose by 2.9 times, of alanine by 2.3 times, cystine by 2.0 times, and glycine by 1.5 times. The concentration of tyrosine fell by half, and that of phenylalanine and serine by 1.4 times. The arginine concentration fell so quickly during perfusion of the liver that by the second hour after the beginning of perfusion no arginine could be found in the medium. The method of amino acid analysis during organ culture as described can be used as a method of developing and correcting culture media.

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Authors
  1. V. A. Barashkov
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  2. I. I. Gitel'zon
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  3. V. P. Nefedov
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  4. I. N. Trubachev
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Barashkov, V.A., Gitel'zon, I.I., Nefedov, V.P. et al. Dynamics of amino acid composition of the medium during culture of isolated liver and kidneys by the controlled perfusion method. Bull Exp Biol Med 80, 1305–1307 (1975). https://doi.org/10.1007/BF00835170

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  • DOI: https://doi.org/10.1007/BF00835170

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