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Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture

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Kidney Organogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1926))

Abstract

Some aspects of renal physiology, in particular transport across tubular epithelia, are highly relevant to pharmacokinetics and to drug toxicity. The use of animals to model human renal physiology is limited, but human-derived renal organoids offer an alternative, relevant system in culture. Here, we explain how the activity of specific transport systems can be assessed in renal organoid and organ culture, using a system illustrated mainly for mouse but that can be extended to human organoids.

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Author information

Authors and Affiliations

  1. Deanery of Biomedical Sciences, University of Edinburgh, Edinburgh, UK

    Melanie L. Lawrence, Mona Elhendawi & Jamie A. Davies

  2. Faculty of Medicine, Clinical Pathology Department, Mansoura University, El-Mansoura, Egypt

    Mona Elhendawi

Authors
  1. Melanie L. Lawrence
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  2. Mona Elhendawi
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  3. Jamie A. Davies
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Corresponding author

Correspondence to Jamie A. Davies .

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Editors and Affiliations

  1. Biocenter Oulu, Laboratory of Developmental Biology, Oulu University, Oulu, Finland

    Seppo Vainio

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Lawrence, M.L., Elhendawi, M., Davies, J.A. (2019). Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture. In: Vainio, S. (eds) Kidney Organogenesis. Methods in Molecular Biology, vol 1926. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9021-4_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9021-4_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9020-7

  • Online ISBN: 978-1-4939-9021-4

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