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Long-term osmotic regulation of amino acid transport systems in mammalian cells

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Summary

Mammalian cells accumulate organic osmolytes, either to adapt to permanent osmotic changes or to mediate cell volume increase in cell cycle progression. Amino acids may serve as osmolytes in a great variety of cells. System A, a transport system for neutral amino acids, is induced after hypertonic shock by a mechanism which requires protein synthesis and gene transcription. Indirect evidence supports the view that system A activity increases due to the interaction of pre-existing A carriers with putative activating proteins. The intracellular accumulation of most neutral amino acids after hypertonic shock depends, exclusively, on the increase in system A activity. Long-term activation of system A is dependent on the integrity of cytoskeletal structures, but in a different way depending on whether cells are polarized or not.

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Authors and Affiliations

  1. Departament de Bioquímica i Biologia Molecular, Facultat de Biologia, Universitat de Barcelona, Diagonal 645, E-08028, Barcelona, Spain

    M. Pastor-Anglada, A. Felipe, F. J. Casado, A. Ferrer-Martínez & M. Gómez-Angelats

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  1. M. Pastor-Anglada
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  2. A. Felipe
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  3. F. J. Casado
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  4. A. Ferrer-Martínez
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  5. M. Gómez-Angelats
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Pastor-Anglada, M., Felipe, A., Casado, F.J. et al. Long-term osmotic regulation of amino acid transport systems in mammalian cells. Amino Acids 11, 135–151 (1996). https://doi.org/10.1007/BF00813857

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  • DOI: https://doi.org/10.1007/BF00813857

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