Abstract
The immunodepressive effect of cyclophosphamide (CP) was studied on mice of three strains (BALB/c, CBA, DBA/2) immunized with sheep's red cells (RBC). When the optimal immunizing dose of antigen was used (5×108 RBC) the strongest immunodepression was observed in the DBA/2 mice, but when a large dose of RBC was used (6.2×109) the strongest effect was observed in DBA/2 and CBA mice. The action of CP was shown to depend on the dose of antigen injected: In BALB/c mice the decrease in the number of antibody-forming cells was the same with both doses of RBC, in the DBA/2 mice an increase in the dose of antigen led to weakening of immunodepression, but in CBA mice immunodepression was intensified (provided that sufficiently large doses of CP were used). Determination of the rate of oxidative hydroxylation of CP by the mouse liver microsomes showed it to be comparatively low in DBA/2 and CBA mice and much higher in BALB/c mice. It is suggested that the differences in the immunodepressive action of CP thus revealed could be due to differences in the sensitivity of the target cells and (or) differences in its metabolism in mice of different strains.
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Pevnitskii, L.A., Telegin, L.Y. & Bol'shev, V.N. Immunodepressive action of cyclophosphamide on different strains of mice. Bull Exp Biol Med 83, 512–515 (1977). https://doi.org/10.1007/BF00807492
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DOI: https://doi.org/10.1007/BF00807492