Skip to main content
SpringerLink
Log in

Effect of tryptophan on rat hepatic nuclear poly(A)polymerase activity

  • Published:
Amino Acids Aims and scope Submit manuscript

Summary

Addition of poly(A) to hnRNA in the cell nucleus is a post-transcriptional event and is presumed to be brought about by a specific poly(A)polymerase. Since it is known that tryptophan rapidly increases the cytoplasmic levels of polyadenylated mRNA, it was of interest to investigate whether the essential amino acid, tryptophan, affects the enzyme responsible for polyadenylation. Tryptophan (300 mg/kg body wt.) tube-fed for 10 min elevated the hepatic nuclear enzymatic activities of both the chromatin-bound nuclear poly(A)polymerase (44%, n = 7) as well as that of the free solubilized form (48%, n = 7). Hepatic nuclear proteins separated under denaturing conditions, transferred to nitrocellulose sheets, and then probed with antibody raised against hepatic nuclear poly(A)polymerase showed no differences between the hepatic nuclei of control and tryptophan-treated rats.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Agutter PS, McArles HJ, McCaldin B (1976) Evidence for involvement of nuclear envelope nucleoside triphosphatase in nucleocytoplasmic translocation of ribonucleoprotein. Nature 263: 165–167

    Google Scholar 

  • Anderson GM, von der Decken A (1975) Deoxyribonucleic acid-dependent ribonucleic acid polymerase activity in rat liver after protein restriction. Biochem J 148: 49–56

    Google Scholar 

  • Blobel G, Potter VR (1966) Nuclei from rat liver: Isolation method that combines purity with high yield. Science 154: 1662–1665

    Google Scholar 

  • Fleck A, Shepherd J, Munro HN (1965) Protein synthesis in rat liver. Influence of amino acids in diet on microsomes and polysomes. Science 150: 628–629

    Google Scholar 

  • Garrett CT, Cairns V, Murty CN, Verney E, Sidransky H (1984) Effect of tryptophan on informosomal and polyribosome-associated messenger RNA in rat liver. J Nutr 114: 50–57

    Google Scholar 

  • Henderson AR (1970) The effect of feeding with a tryptophan-free amino acid mixture on rat liver magnesium ion-activated deoxyribonucleic acid-dependent ribonucleic aciddependent polymerase. Biochem J 120: 205–214

    Google Scholar 

  • Jacob ST, Roe FJ, Rose KM (1976a) Chromatin-bound and free forms of poly(adenylic acid)polymerase in rat hepatic nuclei. Biochem J 1530: 733–735

    Google Scholar 

  • Jacob ST, Rose KM, Munro HN (1976b) Response of poly(adenylic acid)polymerase in rat liver nuclei and mitochondria to starvation and re-feeding with amino acids. Biochem J 158: 161–167

    Google Scholar 

  • Kurl RN, Verney E, Sidransky H (1987) Tryptophan-binding sites on nuclear envelopes of rat liver. Nutr Rep Int 36: 669–677

    Google Scholar 

  • Kurl RN, Holmes SC, Verney E, Sidransky H (1988a) Poly(A)polymerase activity in murine serum. Elevation in animals with proliferative changes. J Natl Cancer Inst 80: 1060–1065

    Google Scholar 

  • Kurl RN, Holmes SC, Verney E, Sidransky H (1988b) Nuclear envelope glycoprotein with poly(A)polymerase activity of rat liver: Isolation, characterization, and immunohistochemical localization. Biochemistry 27: 8974–8980

    Google Scholar 

  • Kurl RN, Verney E, Sidransky H (1988c) Identification and immunohistochemical localization of a tryptophan-binding protein in nuclear envelopes of rat liver. Arch Biochem Biophys 265: 286–293

    Google Scholar 

  • Kurl RN, Barsoum AL, Sidransky H (1992) Association of poly(A)polymerase with tryptophan receptor in rat hepatic nuclei. J Nutr Biochem 3: 366–372

    Google Scholar 

  • Lowry OH, Rosebrough MR, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193: 265–275

    Google Scholar 

  • Matts RL, Siegel FL (1979) Regulation of hepatic poly(A) endonuclease by corticosterone and amino acids. J Biol Chem 254: 11228–11233

    Google Scholar 

  • Murty CN, Verney E, Sidransky H (1977) The effect of tryptophan on nucleocytoplasmic translocation of RNA in rat liver. Biochim Biophys Acta 474: 117–128

    Google Scholar 

  • Murty CN, Verney E, Sidransky H (1979) In vivo and in vitro studies on the effect of tryptophan on translocation of RNA from nuclei of rat liver. Biochem Med 22: 98–109

    Google Scholar 

  • Murty CN, Verney E, Sidransky H (1980) Effect of tryptophan on nuclear envelope nucleoside triphosphatase activity in rat liver. Proc Soc Exp Biol Med 163: 155–161

    Google Scholar 

  • Murty CN, Hornseth R, Verney E, Sidransky H (1983) Effect of tryptophan on enzymes and proteins of hepatic nuclear envelopes of rats. Lab Invest 48: 256–262

    Google Scholar 

  • Rickwood D, Klemperer HG (1970) Decreased ribonucleic acid synthesis on isolated rat liver nuclei during starvation. Biochem J 120: 381–384

    Google Scholar 

  • Sidransky H (1985) Tryptophan: Unique actions by an essential amino acid. In: Sidransky H (ed) Nutritional pathology. Pathobiology of dietary imbalances. Marcel Dekker, New York, pp 1–62

    Google Scholar 

  • Sidransky H, Sarma DSR, Bongiorno M, Verney E (1968) Effect of dietary tryptophan on hepatic polyribosomes and protein synthesis in fasted mice. J Biol Chem 243: 1123–1132

    Google Scholar 

  • Sidransky H, Verney E, Kurl R (1990) Comparison of effects of L-tryptophan and a tryptophan analog, D,L-β-(1-naphthyl)alanine, on processes relating to hepatic protein synthesis in rats. J Nutr 120: 1157–1162

    Google Scholar 

  • Sox HC, Hoagland MB (1966) Functional alterations in rat liver polysomes associated with starvation and refeeding. J Mol Biol 20: 113–121

    Google Scholar 

  • Staehelin T, Verney E, Sidransky H (1967) The influence of nutritional change on polyribosomes of the liver. Biochim Biophys Acta 145: 105–119

    Google Scholar 

  • Vesely J, Cihak A (1970) Enhanced DNA-dependent RNA polymerase and RNA synthesis in rat liver nuclei after administration of L-tryptophan. Biochem Biophys Acta 204: 614–616

    Google Scholar 

  • Wilson SH, Hoagland MB (1967) Physiology of rat-liver polysomes. The stability of messenger ribonucleic acid and ribosomes. Biochem J 103: 556–566

    Google Scholar 

  • Wunner WH, Bell J, Munro HN (1966) The effect of feeding with a tryptophan-free amino acid mixture on rat liver polysomes and ribosomal ribonucleic acid. Biochem J 101: 417–428

    Google Scholar 

Download references

Author information

Author notes

  1. R. N. Kurl

    Present address: Program in Clinical Pharmacology, Brown University, 02912, Providence, RI, USA

Authors and Affiliations

  1. Department of Pathology, The George Washington University Medical Center, 2300 Eye Street, NW, 20037, Washington, DC, USA

    R. N. Kurl, E. Verney & H. Sidransky

Authors
  1. R. N. Kurl
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. Verney
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Sidransky
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This investigation was supported by USPHS Research Grants DK-27339 from the National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases and CA-41832 from the National Cancer Institute.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kurl, R.N., Verney, E. & Sidransky, H. Effect of tryptophan on rat hepatic nuclear poly(A)polymerase activity. Amino Acids 5, 263–271 (1993). https://doi.org/10.1007/BF00805989

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00805989

Keywords

Search

Navigation