Abstract
Incubation of intact liver mitochondria and of mitochondria fragmented by ultrasound for 30 min at 37°C in isolation medium with cholesterol in a concentration of 2×10−3 M leads to the activation of hexokinase, malate dehydrogenase, and glutamate dehydrogenase and inhibits the activity of β-hydroxybutyrate, glycerophosphate, and lactate dehydrogenases. The effect is greater on enzymes of the intact mitochondria.
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Literature Cited
I. V. Ambrozas and P. S. Khomulo, Kardiologiya, No. 8, 39 (1970).
V. Kh. Anestiadi and S. P. Russu, Enzymes of the Arteries and Atherosclerosis [in Russian], Kishinev (1973).
A. N. Klimov, in: Structure, Biosynthesis, and Conversion of Lipids in the Animal and Human Organism [in Russian], Leningrad (1972), p. 72.
M. N. Kondrashova, in: Mitochondria. Structure and Functions under Normal and Pathological Conditions [in Russian], Moscow (1971), p. 25.
M. G. Kritsman and M. V. Bavina, Kardiologiya, No. 9, 5 (1970).
M. N. Pertseva, in: Enzymes in the Evolution of Animals [in Russian], Leningrad (1969), p. 94.
A. Policard and M. Bessis, Elements in Cell Pathology [Russian translation], Moscow (1970).
M. Rashev and D. G. Dobrev, Vutr. Bol.,9, 56 (1970).
G. Beisencherz, T. Bücher, and K. Garbade, Methods Enzymol.,1, 391 (1955).
G. Hogeboom and W. C. Schneider, J. Biol. Chem.,204, 233 (1953).
A. Kornberg, J. Biol. Chem.,1, 441 (1955).
P. Koundakjian and H. M. Snoswell, Biochem. J.,119, 49 (1970).
A. L. Lehninger, H. C. Sudduth, and J. B. Wise, J. Biol. Chem.,235, 2450 (1960).
S. Ochoa, Methods Enzymol.,1, 735 (1955).
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Sidorenkov, I.V., Gil'miyarova, F.N., Radomskaya, V.M. et al. Effect of cholesterol on activity of some mitochondrial enzymes of the liver in vitro. Bull Exp Biol Med 78, 1271–1273 (1974). https://doi.org/10.1007/BF00804355
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DOI: https://doi.org/10.1007/BF00804355