Skip to main content
SpringerLink
Log in

Small nuclear RNAs from Drosophila KC-H cells; characterization and comparison with mammalian RNAs

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Small molecular weight nuclear RNAs were extracted from cultured Drosophila KC-H cells and characterized by their electrophoretic mobilities in 5–15% gradient acrylamide gels or in 10% acrylamide-7 M urea gels. Comparison between the electrophoretic profiles of these SnRNAs with those from human and mouse cells revealed striking similarities and allowed for assignation of band nomenclatures as established for mammalian cells. Comparison of mobilities in the two gel systems also permitted correspondence between the different nomenclatures established by various groups for this class of RNAs, as well as an approximate estimate of their molecular sizes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. AlonsoA., LouisCh., FlytzanisN. & SekerisC. E., 1978. FEBS Letts. 93: 351–356.

    Google Scholar 

  2. AvvedimentoV. E., VogeliG., YoshihikoY., MaizelJ. V., PastanI. & deCombruggheB., 1980. Cell 21: 689–696.

    Google Scholar 

  3. BesterA. J., KennedyD. S. & HeywoodS. M., 1975. Proc. Natl. Acad. Sci. USA 72: 1523–1527.

    Google Scholar 

  4. DeimelB., LouisCH. & SekerisC. E., 1977. FEBS Letts. 73: 80–84.

    Google Scholar 

  5. EliceiriG. L., 1974. Cell 3: 11–14.

    Google Scholar 

  6. EliceiriG. L., 1979. Nature 179: 80–81.

    Google Scholar 

  7. GoldsteinL. & KoC., 1974. Cell 2: 259–266.

    Google Scholar 

  8. GoldsteinL. & TrescottO. H., 1970. Proc. Natl. Acad. Sci. USA 67: 1367–1374

    Google Scholar 

  9. HaradaF. & IkawaY., 1979. Nucl. Acids Res. 7: 895–908.

    Google Scholar 

  10. HaradaF. & KatoW., 1980. Nucl. Acids Res. 8: 1273–1285.

    Google Scholar 

  11. Hellung-LarsenP. & FrederiksenS., 1972. Biochem. Biophys. Acta 262: 290–307.

    Google Scholar 

  12. HerlanG., EckertW., KaffenbergerW. & WunderlichF. 1979. Biochemistry 18: 1782–1788.

    Google Scholar 

  13. HowardE., 1978. Biochemistry 17: 3228–3236.

    Google Scholar 

  14. KnightJr.E. & DarnellJ. E., 1967. J. Mol. Biol. 28: 491–502.

    Google Scholar 

  15. KrauseM. O. & RinguetteM. J., 1977. Biochem. Biophys. Res. Comm. 76: 796–803.

    Google Scholar 

  16. Krause, M. O. & Ringuette, M. J. In: K. S. McCarty & G. M. Padilla (eds.) Genetic Expression in the cell cycle. Genetic Organization. Academic Press, N.Y. In press.

  17. LernerM. R. & SteitzJ. A., 1979. Proc. Nat. Acad. Sci. USA. 76: 5459–5499.

    Google Scholar 

  18. LernerM. R., BoyleJ. A., MountS. M., WolinS. L. & SteitzJ. A., 1980. Nature 283: 220–224.

    Google Scholar 

  19. LiuW. C., GodboutR., JayE., YuK. K-Y. & KrauseM. O., 1981. Can. J. Biochem. 59: 343–352.

    Google Scholar 

  20. MarzluffW. F., WhiteE. L., BenjaminR. & HuangR. C. C., 1975. Biochemistry 14: 3715–3724.

    Google Scholar 

  21. MillerT. E., HuangC. Y. & PogoO., 1978. J. Cell Biol. 76: 692–704.

    Google Scholar 

  22. Nguyen-HuuM. C., SippelA. A., HynesN. E., GronerB. & SchutzG., 1978. Proc. Natl. Acad. Sci. USA 75: 686–690.

    Google Scholar 

  23. NorthemannW., ScheurlanM., GrossV. & HeinrichP. C., 1977. Biochem. Biophys. Res. Comm. 76:1130–1137.

    Google Scholar 

  24. RajN. B. K., Ro-ChoiT.C. & BuschH. 1975. Biochemistry 14: 4380–4385.

    Google Scholar 

  25. Ringuette, M. J., Gordon, K. & Krause, M. O., 1982. Can. J. Biochem. Special Gene replication and expression issue. In press.

  26. RinguetteM. J., LiuW. C., JayE., YuK. K-Y. & KrauseM. O., 1980. Gene 8: 211–224.

    Google Scholar 

  27. Ro-ChoiT. S. & BuschH., 1974. In: H.Busch (ed.) The cell nucleus. Academic Press, N.Y., Vol III pp. 151–208.

    Google Scholar 

  28. RogersJ. & WallR., 1980. Proc. Natl. Acad. Sci. USA. 77: 1877–1879.

    Google Scholar 

  29. WalkerT. A., PaceN. H., FriksonR. I., FriksonT. & BahrF., 1974. Proc. Natl. Acad. Sci. USA 71: 3390–3394.

    Google Scholar 

  30. WeinbergR. A. & PenmanS., 1968. J. Mol. Biol. 38: 286–304.

    Google Scholar 

  31. WeinbergR. A. & PenmanS., 1969. Biochem. Biophys. Acta 190: 10–29.

    Google Scholar 

  32. ZieveG. & PenmanS., 1976. Cell 8: 19–31.

    Google Scholar 

  33. ZieveG., BeneckeB.-J. & PenmanS., 1977. Biochemistry 16: 4521–4525.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Molecular and Microbiology, Department of Biology, University of New Brunswick, E3 B 6E1, Fredericton, N.B., Canada

    L. Ireland, J. Szyszko & M. Krause

Authors
  1. L. Ireland
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Szyszko
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Krause
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ireland, L., Szyszko, J. & Krause, M. Small nuclear RNAs from Drosophila KC-H cells; characterization and comparison with mammalian RNAs. Mol Biol Rep 8, 97–101 (1982). https://doi.org/10.1007/BF00778511

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00778511

Keywords

Search

Navigation