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Biosynthesis of phenolic compounds inVitis vinifera cell suspension cultures: Study on hydroxycinnamoyl CoA:ligase

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An Erratum to this article was published on 21 February 2014

Abstract

In cell suspensions cultures from grape berry pulp (Vitis vinifera cv. Gamay fréaux)hydroxycinnamoyl CoA ligase (CoAL) displayed maximum activity (100 %) forp-coumaric acid and then, in decreasing order, for ferulic acid (81.3 %) and caffeic acid (60.4 %). No activity was detected with sinapic and cinnamic acids. The changes in CoAL activity during the growth cycle of the culture displayed two peaks : the highest (6 h after subculturing) was linked with a strong increase in protein caused by dilution ; the second was weaker and occurred on the 7th day of culture.

Grape cell suspension accumulated mainly peonidin (Pn) and cyanidin (Cy) glucosides (Pn 3-glucoside, Cy 3-glucoside, Pn 3-acetylglucoside, Pn 3-caffeylglucoside, Pn 3-p-coumarylglucoside, and Cy 3-p-coumarylglucoside). Maximum accumulation of anthocyanins was associated with the exponential growth phase of the culture and might be the result of the substantial increase in CoAL activity resulting from the effect of dilution. The second enzyme activity peak was probably oriented towards the acylation of anthocyanins since the percentage of acylated forms increased with time after subculturing.

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Abbreviations

CoAL:

hydroxycinnamoyl-CoA: ligase

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Authors and Affiliations

  1. Laboratoire de Physiologie Végétale Appliquée, Université des Sciences et Techniques du Languedoc, F 34060, Montpellier Cedex, France

    Samia Lofty, Annie Fleuriet, Teresa Ramos & Jean-Jacques Macheix

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  1. Samia Lofty
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  2. Annie Fleuriet
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  3. Teresa Ramos
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  4. Jean-Jacques Macheix
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Additional information

Communicated by W. Barz

An erratum to this article is available at http://dx.doi.org/10.1007/s00299-014-1584-9.

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Lofty, S., Fleuriet, A., Ramos, T. et al. Biosynthesis of phenolic compounds inVitis vinifera cell suspension cultures: Study on hydroxycinnamoyl CoA:ligase. Plant Cell Reports 8, 93–96 (1989). https://doi.org/10.1007/BF00716847

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