Summary
Twelve patients with gliomas received3H-thymidine intraarterially in combination with an i.v. mitostatic agent (Velban: 0.1 mg/kg or 3 mg/M2 of Vincristine) prior to tumor biopsy. The labeling index (LI) of each tumor was compared with the mitotic index divided by the elapsed time from administration of the mitostatic agent to biopsy (MI/t). Most of the malignant gliomas that had an LI of 5–15%, also yielded an MI/t value of 4–5×10−3, whereas tumors with an LI of less than 5% generally showed very slight (0.1×10−3) mitotic accumulation. Use of a mitostatic agent is suggested to differentiate the proliferative activity of various gliomas.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aksiutina, M. S., Lipchina, L. P., Iablonovskla, L. I.: Proliferativnaia aktivnost' neuroglia l'nykh opukholei. Tsitologiia12, 483–496 (1970) (Russian)
Camplejohn, R. S., Aherne, W. A.: In vitro labeling of childhood cancers with tritiated thymidine. Br. J. Cancer29, 487–489 (1974)
Camplejohn, R. S., Bone, G., Aherne, W.: Cell proliferation in rectal carcinoma and rectal mucosa. A stathmokinetic study. Eur. J. Cancer9, 577–581 (1973)
Chigasaki, H.: Studies on the DNA synthesis function of glial cells by means of3H-thymidine microradioautography. Brain Nerve15, 767–781 (1963)
El Dareer, S. M., White, V. M., Chen, F. P., Mellett, L. B., Hill, D. L.: Distribution and metabolism of vincristine in mice, rats, dogs, and monkeys. Cancer Treat. Rep.61, 1269–1277 (1977)
Frei, E., Whang, J., Scoggins, R. B., Van Scott, E. J., Rall, D. P., Ben, M.: The stathmokinetic effect of vincristine. Cancer Res.24, 1918–1925 (1964)
Fukuma, S., Takemoto, S., Ueda, S., Ohmachi, J., Toyama, M., Kitamura, T., Yoshida, S., Maekawa, J., Nakajima, K., Fujita, T.: Autoradiographic studies of brain tumors using local labelling with3H-thymidine in vivo. Brain Nerve21, 1029–1035 (1969)
Hoshino, T., Barker, M., Wilson, C. B., Boldrey, E. B., Fewer, D.: Cell kinetics of human gliomas. J. Neurosurg.37 15–26 (1972)
Hoshino, T., Wilson, C. B.: Review of basic concepts of cell kinetics as applied to brain tumors. J. Neurosurg.42, 123–131 (1975a)
Hoshino, T., Wilson, C. B., Rosenblum, M. L., Barker, M.: Chemotherapeutic implication of growth fraction and cell cycle time in glioblastoma. J. Neurosurg.43, 127–135 (1975b)
Iversen, O. H.: Kinetics of cellular proliferation and cell loss in human carcinomas. A discussion of methods available for in vivo studies. Eur. J. Cancer3, 389–394 (1967)
Johnson, H. A., Haymaker, W. E., Rubini, J. R., Fliedner, T. M., Cronkite, E. P., Hughes, W. L.: A radioautographic study of a human brain and glioblastoma multiforme after the in vivo uptake of tritiated thymidine. Cancer13, 636–642 (1960)
Kleinfeld, R. G., Sisken, J. E.: Morphological kinetic aspects of mitotic arrest by and recovery from colcemide. J. Cell Biol.31, 369–379 (1966)
Kury, G., Carter, H. W.: Autoradiographic study of human nervous system tumors. Arch. Pathol.80, 38–42 (1965)
Meyer, J. S., Donaldson, R. C.: Growth kinetics of squamous cell carcinoma in man. A study of four squamous cell carcinomas using stathmokinetic effect of vinblastine in vivo. Arch. Pathol.87, 479–490 (1969).
Puck, T., Steffen, J.: Life cycle analysis of mammalian cells. I.A method for localizing metabolic events within the life cycle, and its application to the action of colcemide and sublethal doses of x-irradiation. Biophys. J.3, 379–397 (1963)
Refsum, S. B., Berdal, P.: A clinical experimental investigation of the rate of cell proliferation in human malignant tumours. Acta Otolaryngol. (Stockh.)67, 101–106 (1969)
Rubini, J. R., Cronkite, E. P., Bond, V. P., Fliedner, T. M.: The metabolism and fate of tritiated thymidine in man. J. Clin. Invest.39, 909–918 (1960)
Shepstone, B. J., Sealy, R., Greenstein, A., Rapley, L. F.: Potential doubling times of human squamous carcinomas as measured by vincristine. Acta Radiol.13, 153–166 (1974)
Steel, G. G.: Cell loss from experimental tumours. Cell Tissue Kinet.1, 193–207 (1968)
Tannock, I. F.: A comparison of the relative efficiencies of various metaphase arrest agents. Exp. Cell Res.47, 345–356 (1967)
Tym, R.: Distribution of cell doubling times in in vivo human cerebral tumors. Surg. Forum20, 445–447 (1969)
Wagner, H. P., Swindzinska, P. M., Hirt, A.: Variable duration of vincristine induced metaphase block in leukemic and normal bone marrow cells of children. Med. Pediatr. Oncol.3, 75–83 (1977)
Wright, N. A., Britton, D. C., Bone, G., Appleton, D. R.: An in vivo stathmokinetic study of cell proliferation in human gastric carcinoma and gastric mucosa. Cell Tissue Kinet.10, 429–436 (1977)
Author information
Authors and Affiliations
Additional information
This work was supported in part by grants CA-13525, CA-19992 from the National Cancer Institute and a gift from the Association for Brain Tumor Research
Rights and permissions
About this article
Cite this article
Hoshino, T., Wilson, C.B. & Muraoka, I. The stathmokinetic (Mitostatic) effect of vincristine and vinblastine on human gliomas. Acta Neuropathol 47, 21–25 (1979). https://doi.org/10.1007/BF00698268
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00698268