Skip to main content
Log in

Fatty acid pattern of haemolymph lipids inSpodoptera littoralis Boisduval larvae

  • Published:
Journal of comparative physiology Aims and scope Submit manuscript

Summary

  1. 1.

    Gas-liquid chromatographic analyses of haemolymph lipids inSpodoptera littoralis Boisduval larvae showed the presence of C12:0, C14br, C14:0, C15:0, C15:1, C16:0, C16:1, C17:0, C18:0, C18:1, C18:2, and C18:3 fatty acids.

  2. 2.

    In all studied instars, palmitic acid was the predominant composing about 46–56 per cent of the total, while oleic acid was the major unsaturated fatty acid comprising about 17–19 per cent of all acids.

  3. 3.

    The relative amount of monounsaturated acids was about two and half times more than the polyunsaturated group in the third, fourth, and sixth instars and about four times more in the fifth instar.

  4. 4.

    Relative per cent of linolenic acid was higher in the sixth instar than that of the other studied instars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Babcock, K. L., Rutschky, C. W.: Lipids in insect eggs: A review with new evidence from the milk-weed bug, Oncopeltus fasciatus. Ann. entomol. Soc. Amer.54, 156–164 (1961)

    Google Scholar 

  • Beenakhers, A. M. Th., Gilbert, L. I.: The fatty acid composition of fat body and haemolymph lipids in Hyalophora cecropia and its relation to lipid release. J. Insect Physiol.14, 481–494 (1968)

    Google Scholar 

  • Boctor, I. Z., Salem, S. I.: Free amino acids of the haemolymph of the cotton leaf-worm,Spodoptera littoralis Boisduval larvae (Lepidoptera: Noctuidae). Comp. Biochem. Physiol.45 B, 785–790 (1973)

    Google Scholar 

  • El-Ibrashy, M. T., Chenouda, M. S.: Cholinesterase activity inSpodoptera littoralis Boisd., in relation to development and metamorphosis. Z. angew. Ent.65, 146–156 (1970)

    Google Scholar 

  • Folch, J., Lees, M., Sloane-Stanley, G. H.: A simple method for isolation and purification of total lipids from animal tissues. J. biol. Chem.226, 497–509 (1957)

    Google Scholar 

  • Gilbert, L. I.: Lipid metabolism and function in insects. In: Advances in insect physiology (J. W. L. Beament, J. E. Treherne, and V. B. Wigglesworth, eds.), vol. 4, pp. 69–211. New York-London: Academic Press 1967

    Google Scholar 

  • Gilbert, L. I., Chino, H., Domroese, K. A.: Lipolytic activity of insect tissues and its significance in lipid transport. J. Insect Physiol.11, 1057–1070 (1965)

    Google Scholar 

  • Gilmour, D.: The biochemistry of insects. New York: Academic Press (1961)

    Google Scholar 

  • Horning, E. C., Ahrens, E. H., Lipsky, S. R., Mattson, F. H., Mead, J. F., Turner, D. A., Goldwater, W. H.: Quantitative analysis of fatty acids by gas-liquid chromatography. J. Lipid Res.5, 20–27 (1964)

    Google Scholar 

  • Luddy, F. E., Barford, R. A., Riemenschneider, R. W.: Direct conversion of lipid components to their fatty acid methyl esters. J. Amer. Oil Chemists' Soc.37, 447–451 (1960)

    Google Scholar 

  • Nelson, D. R., Terranova, A. C., Sukkestad, D. R.: Fatty acid composition of the glyceride and free fatty acid fractions of the fat body and haemolymph of the cockroach, Periplaneta americana (L.). Comp. Biochem. Physiol.20, 907–917 (1967)

    Google Scholar 

  • Nicolaysen, R., Nygaard, A. P.: The determination of triglycerides and phospholipids. Scand. J. clin. Lab. Invest.15, 79–82 (1963)

    Google Scholar 

  • Niemierko, W.: Some aspects of lipid metabolism in insects. Fourth Int. Congr. Biochem.12, 185–197 (1959)

    Google Scholar 

  • Pearincott, J. V.: Changes in lipid content during growth and metamorphosis of the house fly, Musca domestica Linnaeus. J. cell. comp. Physiol.55, 167–174 (1960)

    Google Scholar 

  • Richeson, J. S., Nation, J. L., Wilkinson, R. C.: Fatty acid composition of Ips calligraphus (Coleoptera: Scolytidae) during postembryonic development. Ann. entomol. Soc. Amer.64, 251–254 (1971)

    Google Scholar 

  • Thompson, S. N., Barlow, J. S.: Presence and synthesis of a 20 carbon monounsaturated fatty acid, 9-eicosenoic acid, and other fatty acids, in Galleria mellonella (Lepidoptera: Pyralidae) Ann. entomol. Soc. Amer.65, 1020–1023 (1972)

    Google Scholar 

  • Wang, C. M., Patton, R. L.: Lipids in the haemolymph of the cricket, Acheta domesticus. J. Insect Physiol.15, 851–860 (1969)

    Google Scholar 

  • Yendol, W. G.: Fatty acid composition of Galleria larvae, haemolymph, and diet (Lepidoptera: Galleriidae). Ann. entomol. Soc. Amer.63, 339–341 (1970)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Boctor, I.Z., Jwanny, E.W. Fatty acid pattern of haemolymph lipids inSpodoptera littoralis Boisduval larvae. J Comp Physiol B 93, 151–155 (1974). https://doi.org/10.1007/BF00696269

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00696269

Keywords

Navigation