Summary
Relatively pure mitochondrial-free Sarcoplasmic reticulum (SR) was isolated from crab and cockroach skeletal muscle, and the SR calcium uptake was studied in control and insecticide-treated conditions by incubation in Ca45 media followed by millipore filtration and counting by liquid scintillation methods. All insecticides used (parathion, DDT, γBHC and tri-cresyl phosphate) at 1 mM concentrations caused a slight fall in the intrinsic Ca2+ content of the SR, but this Ca2+ loss was insufficient to explain the contracture induction effect of these agents.
All insecticides used (at 1 mM) caused a massive inhibition of SR Ca2+ uptake, and all were able to release Ca2+ previously bound to the SR during incubation in the presence of ATP. It is concluded that the contracture-induction effect of these insecticides is due to their inhibition of SR Ca2+ uptake, resulting in a massive rise in myoplasmic free Ca2+.
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Huddart, H., Greenwood, M. & Williams, A.J. The effect of some organophosphorous and organochlorine compounds on calcium uptake by Sarcoplasmic reticulum isolated from insect and crustacean skeletal muscle. J Comp Physiol B 93, 139–150 (1974). https://doi.org/10.1007/BF00696268
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DOI: https://doi.org/10.1007/BF00696268