Summary
The effect of the carbonic anhydrase inhibitor Acetazolamide on the response of worker honeybee antennal CO2-receptors to CO2 and N2O was tested by extracellular recordings of spike activity from single cells.
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1.
Within 15 minutes after oral application of Acetazolamide the spike frequency of the response to test stimuli of constant CO2-concentration decreases to a lower but constant value.
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2.
Stimulus-response curves measured after subsequent applications of increasing Acetazolamide doses are shifted towards higher CO2-concentrations to an extent which is related to the Acetazolamide dose.
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3.
The effect of Acetazolamide on the phasic peak of the CO2-response is more pronounced than the effect on the plateau.
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4.
The response to N2O, which is antagonistic to the CO2-response, is not affected by the presence of Acetazolamide.
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5.
When a CO2-stimulus of high concentration is applied in the presence of a very high Acetazolamide concentration, a response is observed which indicates that CO2 also can cause an effect of the same kind as N2O.
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6.
It is concluded that the effect of Acetazolamide results from its inhibitory action on a molecular acceptor with a CO2-binding site similar to that in carbonic anhydrase.
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7.
It is suggested that the responses to N2O and Xe and also the antagonistic component of the response to high concentrations of CO2 result from the action of those gases on a secondary step in the chain of sensory transduction.
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Stange, G. The influence of a carbonic anhydrase inhibitor on the function of the honeybee antennal CO2-receptors. J. Comp. Physiol. 91, 147–159 (1974). https://doi.org/10.1007/BF00694270
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DOI: https://doi.org/10.1007/BF00694270