Summary
The temperature dependence of egg laying was examined in winter-caughtAplysia. Cold-waterAplysia californica and warm-waterA. brasiliana were individually housed in the same large aquarium for 16 days at 15°C, and then for 16 days at 20°C. Initially, the majority of theA. californica were not reproductively mature (as determined by injections of atrial gland extracts) whereas all of theA. brasiliana were reproductively mature. When the temperature was increased from 15 to 20°C, both species showed a marked increase in the frequency of egg laying. At both temperatures,A. brasiliana laid eggs more frequently but produced smaller egg masses thanA. californica. We conclude that increased egg laying inA. californica was attibutable both to facilitation of oogenesis in previously reproductively immature animals and to increased activity of the bag cells which release an egg-laying hormone. Increased egg laying inA. brasiliana was attributable primarily to increased bag cell activity.
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References
Arch S (1972) Biosynthesis of the egg-laying hormone (ELH) in the bag cell neurons ofAplysia californica. J Gen Physiol 60:102–119
Arch S, Smock T, Gurvis R, McCarthy C (1978) Atrial gland induction of the egg-laying response inAplysia californica. J Comp Physiol 128:67–70
Arch S, Lupatkin J, Smock T, Beard M (1980) Evidence for an exocrine function of theAplysia atrial gland. J Comp Physiol 151:131–137
Audesirk TE (1979) A field study of growth and reproduction inAplysia californica. Biol Bull 157:407–421
Berry RW (1982) Seasonal modulation of synthesis of the neurosecretory egg-laying hormone ofAplysia. J Neurobiol 13:327–335
Berry RW (1984) Environmental temperature modulates the rate of synthesis of egg-laying hormone inAplysia. J Comp Physiol B 154:545–548
Blankenship JE (1980) Physiological properties of peptidesecreting neuroendocrine cells in the marine mollusc,Aplysia. In: Barker JJ, Smith TG (eds) Role of peptides in neuronal function. Marcel Dekker, New York, pp 159–187
Blankenship JE, Rock MK, Schlesinger DH (1982) Structure and function of peptides from a neuroendocrine system controlling egg-laying behavior inAplysia. In: Haber B, Perez-Polo JR, Coulter JD, (eds) Proteins of the nervous system: structure and function. Alan R. Liss, Inc., New York, pp 159–177
Carefoot TH (1967) Growth and nutrition ofAplysia punctata feeding on a variety of marine algae. J Mar Biol Assoc (UK) 47:565–589
Carefoot TH (1970) A comparison of absorption and utilization of food energy in two species of tropicalAplysia. J Exp Mar Biol Ecol 4:47–62
Carpenter DO (1967) Temperature effects on pacemaker generation, membrane potential, and critical firing threshold inAplysia neurons. J Gen Physiol 50:1469–1484
Coggeshall RE (1970) A cytologic analysis of the bag cell control of egg laying inAplysia. J Morphol 132:461–485
Dudek FE, Soutar B, Tobe SS (1980) Reproduction inAplysia californica: correlation between egg laying in vivo and egg release in vitro. Can J Zool 58:2163–2166
Feinstein R, Pinsker H, Schmale M, Gooden BA (1977) Bradycardial response inAplysia exposed to air. J Comp Physiol 122:311–324
Ferguson GP, Parsons DW, ter Maat A, Pinsker H (1984) Bag cell activity and egg laying inAplysia. Neurosci Abstr 10:150
Gev S, Archituv Y, Susswein AJ (1984) Seasonal determinants of the life cycle in two species ofAplysia found in shallow waters along the Mediterranean coast of Israel. J Exp Mar Biol Ecol 74:67–83
Heller E, Kaczmarek L, Hunkapillar M, Hood L, Strumwasser F (1980) Purification and primary structure of two neuroactive peptides that cause bag cell afterdischarge and egg-laying inAplysia. Proc Natl Acad Sci (USA) 77:2328–2332
Kandel ER (1979) Behavioral Biology ofAplysia. Freeman Publ Co, San Franciso
Kupfermann I (1967) Stimulation of egg-laying: possible neuroendocrine function of bag cells of abdominal ganglion ofAplysia californica. Nature 216:814–815
Kupfermann I (1970) Stimulation of egg laying by extracts of neuroendocrine cells (bag cells) of abdominal ganglion ofAplysia. J Neurophysiol 33:877–881
Kupfermann I, Kandel ER (1970) Electrophysiological properties and functional interconnections of two symmetrical neurosecretory clusters (bag cells) in abdominal ganglion ofAplysia. J Neurophysiol 33:865–876
Marchiafava PL (1970) The effect of temperature change on membrane potential and conductance inAplysia giant nerve cell. Comp Biochem Physiol 34:847–852
Moffett S, Wachtel H (1976) Correlations between temperature effects on behavior inAplysia and firing patterns of identified neurons. Mar Behav Physiol 4:61–74
Murray RW (1966) The effect of temperature on the membrane properties of neurons in the visceral ganglion ofAplysia. Comp Biochem Physiol 18:291–303
Nagle GT, Painter SD, Blankenship JE (1982) Effects of atrial gland peptides on bag cells and ovotestis in intactAplysia. Soc Neurosci Abstr 8:987
Peretz B, Adkins L (1982) An index of age when birthdate is unknown inAplysia californica: shell size and growth in long-term maricultured animals. Biol Bull 162:333–344
Pinsker HM (1977)Aplysia bursting neurons as endogenous oscillators. I. Phase-response curves for pulsed inhibitory synaptic input. J Neurophysiol 40:527–556
Pinsker HM, Dudek FE (1977) Bag cell control of egg-laying in freely behavingAplysia. Science 197:490–493
Pinsker H, Feinstein R, Sawada M, Coggeshall R (1976) Anatomical basis for an apparent paradox concerning conduction velocities of two identified axons inAplysia. J Neurobiol 7:241–253
Schlesinger DH, Babirak SP, Blankenship JE (1981) Primary structure of an egg laying peptide from the atrial gland ofAplysia californica. In: Schlesinger DH (ed) Symposium on Neurohypophyseal Peptide Hormones and other Biologically Active Peptides. Elsevier North-Holland, New York, pp 137–150
Siegel S (1956) Nonparametric statistics. McGraw Hill, New York
Smith ST, Carefoot TH (1967) Induced maturation of gonads inAplysia punctata Cuvier. Nature 215:652–653
Stuart DK, Chiu AU, Strumwasser F (1980) Neurosecretion of egg-laying hormone and other peptides from electrically active bag cell neurons ofAplysia. J Neurophysiol 43:488–498
Strumwasser F, Jacklet JW, Alvarez RB (1969) A seasonal rhythm in the neural extract induction of egg-laying inAplysia. Comp Biochem Physiol 29:197–206
von der Porten K, Parsons DW, Rothman BS, Pinsker HM (1982) Swimming inAplysia brasiliana: analysis of behavior and neuronal pathways. Behav Neural Biol 36:1–23
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Pinsker, H.M., Parsons, D.W. Temperature dependence of egg laying inAplysia brasiliana andA. californica . J Comp Physiol B 156, 21–27 (1985). https://doi.org/10.1007/BF00692922
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DOI: https://doi.org/10.1007/BF00692922