Summary
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1.
Carbon dioxide dissociation curves were determined at 15°C on the oxygenated and deoxygenated sera of nine specimens of the shore crabCarcinus maenas, at various stages of the molting cycle.
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2.
These curves vary in shape and position, being steeper at intermolt and premolt stages when the blood protein concentration is high, than at postmolt stages, characterized by low blood protein concentration.
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3.
The buffering power of non-bicarbonate buffers, defined as the in vitro slope of the [HCO −3 ]vs. pH line at variable\(P_{CO_2 } \) and constant base, varies linearly with the protein concentration and the oxygen capacity.
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4.
A small but typical haldane effect is present at alkaline pH,\(C_{CO_2 } \) and pH being higher for a given\(P_{CO_2 } \) in the deoxygenated serum.
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References
Albers, C., Pleschka, K.: Effect of temperature on CO2 transport in elasmobranch blood. Resp. Physiol.2, 261–273 (1967)
Barlow, J., Ridgway, G.J.: Changes in serum protein during the molt and reproductive cycles of the American lobster (Homarus americanus). J. Fish. Res. Bd, Canada26, 2101–2109 (1969)
Burton, R.F.: The role of buffers in body fluids: mathematical analysis. Resp. Physiol.18, 34–42 (1973)
Busselen, P.: Effect of moulting cycle and nutritional conditions on hemolymph proteins inCarcinus maenas. Comp. Biochem. Physiol.37, 73–83 (1970)
Christiansen, J., Douglas, C.C., Haldane, J.S.: The absorption and dissociation of carbon dioxide by human blood. J. Physiol. (Lond.)48, 244–277 (1914)
Davenport, H.W.: The ABC of acid-base chemistry, 4th ed. University of Chicago Press 1958
Drach, P.: Mue et cycle d'intermue chez les Crustacés Décapodes. Ann. Inst. Océanogr. Monaco, N.S.19, 103–391 (1939)
Drach, P., Tchernigovtzeff, C.: Sur la méthode de détermination des stades d'intermue et son application générale aux Crustacés. Vie et Milieu18A, 595–607 (1967)
Drach, P., Teissier, G.: Mue et protidémie chez les Crabes. C.R. Soc. Biol. (Paris)131, 1199–1201 (1939)
Drilhon, A.: Etude biochimique de la mue chez les Crustacés Brachyoures (Maïa squinado). Ann. Physiol. Physicochim. biol.11, 301–326 (1935a)
Drilhon, A.: La réserve alcaline, le pH et la mue des Crustacés Brachyoures. C.R. Acad. Sci. (Paris)200, 1150–1152 (1935b)
Evans, P.D.: The free aminoacid pool of the hemocytes ofCarcinus maenas. J. exp. Biol.56, 501–507 (1972)
Florkin, M.: Sur l'activité anhydrasique du milieu intérieur des Invertébrés. Arch. intern. Physiol.40, 283–290 (1935)
Greenaway, P.: Total body calcium and haemolymph calcium concentrations in the crayfishAustropotamobius pallipes (Lereboullet). J. exp. Biol.61, 19–26 (1974)
Johansen, K., Lenfant, C., Mecklenburg, T.A.: Respiration in the crab,Cancer magister. Z. vergl. Physiol.70, 1–19 (1970)
Levin, R., Brauer, R.W.: The biuret reaction for the determination of protein. An improved reagent and its application. J. Lab. clin. Med.38, 474–480 (1951)
Manwell, C.: Comparative physiology: blood pigments. Ann. Rev. Physiol.22, 191–244 (1960)
Parsons, T.R., Parsons, W.: Observations on the transport of carbon dioxide in the blood of some marine invertebrates. J. gen. Physiol.6, 153–156 (1923)
Piiper, J., Baumgarten-Schumann, D.: Transport of O2 and CO2 by water and blood in gas exchange of the dogfish (Scyliorhinus stellaris). Resp. Physiol.5, 326–337 (1968)
Piiper, J., Dejours, P., Haab, P., Rahn, H.: Concepts and basic quantities in gas exchange physiology. Resp. Physiol.13, 292–304 (1971)
Redmond, J.R.: The respiratory function of hemocyanin in Crustacea. J. cell. comp. Physiol.46, 209–247 (1955)
Robertson, J.D.: Some features of the calcium metabolism of the shore crab (Carcinus maenas Pennant). Proc. roy. Soc. B124, 162–182 (1937)
Roughton, F.J.W., Scholander, P.F.: Microgasometric estimation of blood gases. I. Oxygen. J. biol. Chem.148, 541–550 (1943)
Siggaard-Andersen, O.: The acid-base status of blood. Scand. J. clin. lab. Invest15, Suppl. 10, 1–134 (1963)
Slyke, D.D. van: On the measurement of buffer value and on the relationship of buffer value to the dissociation constant of the buffer and the concentration and reaction of the buffer. J. biol. Chem.52, 525–570 (1922)
Travis, D.F.: The moulting cycle of the spiny lobsterPanulirus argus Latreille. III. Physiological changes which occur in the blood and urine during the normal molting cycle. Biol. Bull. mar. biol. Lab., Woods Hole109, 484–503 (1955)
Truchot, J.P.: Action de l'hypercapnie sur l'état acide-base du sang chez le crabeCarcinus maenas. C.R. Acad. Sci. (Paris)272, 984–987 (1971)
Truchot, J.P.: Temperature and acid-base regulation in the shore crabCarcinus maenas (L.). Resp. Physiol.17, 11–20 (1973a)
Truchot, J.P.: Action spécifique du dioxyde de carbone sur l'affinité pour l'oxygène de l'hémocyanine deCarcinus maenas (L.) (Crustacé Décapode Brachyoure). C.R. Acad. Sci. (Paris)276, 2965–2968 (1973b)
Truchot, J.P.: Fixation et transport de l'oxygène par le sang deCarcinus maenas: variations en rapport avec diverses conditions de température et de salinité. Neth. J. Sea Res.7, 482–495 (1973c)
Truchot, J.P.: Le transport des gas respiratoires (oxygène et dioxyde de carbone) par le sang du crabeCarcinus maenas (L.). Thèse de Doctorat es Sciences, Université de Paris VI, No CNRS AO 9817 (1974)
Truchot, J.P.: Blood acid-base changes during experimental emersion and reimmersion of the shore crabCarcinus maenas (L.). Resp. Physiol.23, 351–360 (1975a)
Truchot, J.P.: Action de l'hypercapnie sur l'état acide-base du sang chez le crabeCarcinus maenas (L.) (Crustacé Décapode). C.R. Acad. Sci. (Paris)280, 311–314 (1975b)
Truchot, J.P.: Changements de l'état acide-base du sang en fonction de l'oxygénation de l'eau chez le crabeCarcinus maenas. J. Physiol. (Paris)70, 583–592 (1975c)
Truchot, J.P.: Carbon dioxide combining properties of the blood of the shore crabCarcinus maenas (L.): carbon dioxide solubility coefficient and carbonic acid dissociation constants. J. exp. Biol.64, 45–57 (1976)
Uglow, R.F.: Haemolymph protein concentrations in portunid crabs. I. Studies on adultCarcinus maenas. Comp. Biochem. Physiol.30, 1083–1090 (1969a)
Uglow, R.F.: Haemolymph protein concentrations in portunid crabs. II. The effects of imposed fasting onCarcinus maenas. Comp. Biochem. Physiol.31, 959–967 (1969b)
Wolvekamp, H.P., Kruyt, W.: Experiments on the carbon dioxide transport by the blood of the edible snail (Helix pomatia), the common crab (Cancer pagurus) and the common lobster (Homarus vulgaris). Arch. néerl. Physiol.28, 620–629 (1948)
Wolvekamp, H.P., Waterman, T.H.: Respiration. In: The physiology of Crustacea, Vol. I (Waterman, T.H., ed.), pp. 35–100, New York and London: Academic Press 1960
Wyman, J.: Heme proteins. Advanc. Protein Chem.4, 407–531 (1948)
Zuckerkandl, E.: Hémocyanine et cuivre chez un Crustacé Décapode dans leurs rapports avec le cycle d'intermue. Ann. Inst. Océanogr. Monaco38, 1–122 (1960)
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Truchot, J.P. Carbon dioxide combining properties of the blood of the shore crab,Carcinus maenas (L.): CO2-dissociation curves and Haldane effect. J Comp Physiol B 112, 283–293 (1976). https://doi.org/10.1007/BF00692299
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DOI: https://doi.org/10.1007/BF00692299