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Adenylate cyclase in the microvessels of the rat brain

A histochemical study with light and electron microscopy

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Summary

The presence of adenylate cyclase (AC) in the microvessels of the rat brain was studied by a new histochemical method for light and electron microscopy. The method is based on the precipitation of strontium and the subsequent conversion of the formed strontium salt into lead phosphate. Isoproterenol and 5-guanylylimidodiphosphate were used as enzyme activators. In the light microscope, the final reaction product was detected in the choroid plexus as well as in the walls of the microvessels in the brain parenchyma. In the electron microscope, both the luminal and abluminal endothelial membrane as well as the basal lamina of the parenchymal microvessels displayed reaction product. The observations demonstrate that isoproterenol-stimulated AC is located in the endothelium of the rat brain microvessels and suggest that the enzyme may play a role in the receptor-mediated regulation of endothelial functions.

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Authors and Affiliations

  1. Dept. of Neuropathology, Medical Research Center, Polish Academy of Sciences, 3 Dworkowa Str., PL-00-784, Warsaw, Poland

    G. Szumańska

  2. Dept. of Anatomy, Eye Research Laboratory, University of Helsinki, Siltavuorenpenger 20 A, SF-00170, Helsinki 17, Finland

    A. Palkama, J. I. Lehtosalo & H. Uusitalo

Authors
  1. G. Szumańska
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  2. A. Palkama
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  3. J. I. Lehtosalo
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  4. H. Uusitalo
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Supported by a grant from the Finnish Academy

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Szumańska, G., Palkama, A., Lehtosalo, J.I. et al. Adenylate cyclase in the microvessels of the rat brain. Acta Neuropathol 62, 219–224 (1984). https://doi.org/10.1007/BF00691855

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  • DOI: https://doi.org/10.1007/BF00691855

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