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Cellular volume regulation in salinity stressed molluscs: The response ofNoetia ponderosa (Arcidae) red blood cells to osmotic variation

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Summary

Salinity stressed marine molluscs volumeregulate utilizing intracellular free amino acids. However, the actual changes in cell volume associated with free amino acid regulation are usually nerver measured or only indirectly measured since volume regulation has only been studied in whole animals or isolated tissues. The blood cells ofNoetia ponderosa (Mollusca: Arcidae) were used to specify the relationship between cell size changes and free amino acid permeability during volume regulation. Over the nonlethal salinity range,N. ponderosa is an osmotic conformer that volume regulates using free amino acids. Furthermore, when changes in blood cell size were measured with hematocrits, isolatedN. ponderosa blood cells placed into hypoosmotic seawater, volume regulated with a free amino acid efflux. Thus, the isolatedN. ponderosa blood cells provide a suitable preparation for studying the control of membrane amino acid permeability during volume regulation.

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Abbreviations

FAA :

free amino acids

ASW :

artificial seawater

NPS :

ninhydrin positive substances

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Author notes

  1. Lynn M. Amende

    Present address: National Institute for Neurological and Communicative Disorders and Stroke, National Institutes of Health, Building 36 Room 4D20, 20205, Bethesda, Maryland, USA

Authors and Affiliations

  1. Department of Zoology, University of Maryland, 20742, College Park, Maryland, USA

    Lynn M. Amende & Sidney K. Pierce

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  1. Lynn M. Amende
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  2. Sidney K. Pierce
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Amende, L.M., Pierce, S.K. Cellular volume regulation in salinity stressed molluscs: The response ofNoetia ponderosa (Arcidae) red blood cells to osmotic variation. J Comp Physiol B 138, 283–289 (1980). https://doi.org/10.1007/BF00691562

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