Summary
With cells which have been grown at 20°C, the circadian rhythm of bioluminescence inGonyaulax polyedra disappears at a critical temperature, which is about 12°C. The transition from the rhythmic to the arrhythmic state is very sharp with temperature: the two states are separated by only 1–2°C. Following a return to a higher temperature (20°C) under otherwise constant conditions, the rhythm resumes with its new phase defined by the time of the cool to warm transition. Loss of rhythmicity also occurs in constant bright light, with a similar resumption and phase determination upon transfer to darkness. The experiments described here show that the effects of light and low temperature are additive: rhythmicity is lost under combined low temperature and light intensity treatments which are ineffective individually.
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Abbreviations
- CT:
-
circadian time
- ft-c:
-
footcandle
- LD 12:12:
-
12 h light/12 h dark cycle
References
Ball, N.G., Dyke, I.J.: The effects of decapitation, lack of oxygen, and low temperature on the endogenous 24 h rhythm in the growth-rate ofAvena coleoptile. J. Exp. Bot.8, 323–338 (1957)
Bruce, V.G.: Environmental entrainment of circadian rhythms. Cold Spring Harbor Symp. Quant. Biol.25, 29–48 (1960)
Bünning, E.: The physiological clock, 3rd English ed., pp. 258. Berlin-Heidelberg-New York: Springer 1973
Eckert, R.: Excitation and luminescence inNoctiluca miliaris. In: Bioluminescence in progress (eds. F.H. Johnson, Y. Haneda), pp. 269–300. Princeton: Princeton Univ. Press 1966
Fogel, M., Hastings, J.W.: A substrate binding molecule in theGonyaulax bioluminescence reaction. Arch. Biochem. Biophys.142, 310–321 (1971)
Hastings, J.W.: Biochemical aspects of rhythms: Phase shifting by chemicals. Cold Spring Harbor Symp. Quant. Biol.25, 131–144 (1960)
Hastings, J.W.: The role of light in persistent daily rhythms. In: Photophysiology: Action of light on living materials, Vol. 1 (ed. A.C. Giese), pp. 333–361. New York: Academic Press 1964
Hastings, J.W.: Are circadian rhythms conditional? Int. J. Chronobiol.1, 329 (1973)
Hastings, J.W., Bode, V.C.: Biochemistry of rhythmic systems. Ann. New York Acad. Sci.98, 876–889 (1962)
Hastings, J.W., Sweeney, B.M.: On the mechanism of temperature independence in a biological clock. Proc. Nat. Acad. Sci.43, 804–811 (1957)
Hastings, J.W., Sweeney, B.M.: A persistent diurnal rhythm of luminescence inGonyaulax polyedra. Biol. Bull.115, 440–458 (1958)
Hesse, M.: Der Einfluß niedriger Temperatur auf die endogene Rhythmik der Reproduktionsfähigkeit vonChlorella. Z. Pflanzenphysiol.71, 428–436 (1974)
Johnsson, A., Karlsson, H.G.: A feedback model for biological rhythms. I. Mathematical description and basic properties of the model. J. Thoer. Biol.36, 153–174 (1972)
McDaniel, M., Sulzman, F.M., Hastings, J.W.: Heavy water slows theGonyaulax clock: A test of the hypothesis that D2O affects circadian oscillations by diminishing the apparent temperature. Proc. Nat. Acad. Sci.71, 4389–4391 (1974)
McMurry, L.M.: Studies on properties and biochemistry of circadian rhythms in the bioluminescent dinoflagellate,Gonyaulax polyedra. Ph.D. thesis, Harvard University, Cambridge, Massachusetts 1971
Njus, D.: The control of bioluminescence inGonyaulax polyedra. Ph.D. thesis, Harvard University, Cambridge, Massachusetts 1975
Njus, D., Gooch, V.D., Mergenhagen, D., Sulzman, F., Hastings, J.W.: Membranes and molecules in circadian systems. Fed. Proc.35, 2353–2357 (1976)
Njus, D., Sulzman, F.M., Hastings, J.W.: Membrane model for the circadian clock. Nature248, 116–120 (1974)
Pittendrigh, C.S.: On temperature independence in the clock-system controlling emergence time inDrosophila. Proc. Nat. Acad. Sci.40, 1018–1029 (1954)
Pittendrigh, C.S.: Circadian rhythms and the circadian organization of living systems. Cold Spring Harbor Symp. Quant. Biol.25, 159–184 (1960)
Pittendrigh, C.S.: On the mechanism of the entrainment of a circadian rhythm by light cycles. In: Circadian clocks (ed. J. Aschoff), pp. 277–297. Amsterdam: North-Holland 1965
Pittendrigh, C.S.: Circadian oscillations in cells and the circadian organization of multicellular systems. In: The neurosciences: Third study program (eds. F.O. Schmitt, F.G. Worden), pp. 437–458. Cambridge, Mass.: M.I.T. Press 1974
Pittendrigh, C.S.: Circadian clocks: What are they? In: The molecular basis of circadian rhythms (eds. J.W. Hastings, H.-G. Schweiger), pp. 11–48. Berlin: Abakon Verlagsgesellschaft 1976
Roberts, S.K. deF.: Circadian activity rhythms in cockroaches. II. Entrainment and phase shifting. J. Cell. Comp. Physiol.59, 175–186 (1962)
Sweeney, B.M., Hastings, J.W.: Characteristics of the diurnal rhythm of luminescence inGonyaulax polyedra. J. Cell. Comp. Physiol.49, 115–128 (1957)
Sweeney, B.M., Hastings, J.W.: Effects of temperature upon diurnal rhythms. Cold Spring Harbor Symp. Quant. Biol.25, 87–104 (1960)
Wagner, E.: Der Einfluß niedriger Temperatur auf die Phasenlage der endogen-tagesperiodischen Blattbewegungen vonPhaseolus multiflorus. Z. Bot.51, 179–204 (1963)
Winfree, A.T.: Integrated view of resetting a circadian clock. J. Theoret. Biol.28, 327–374 (1970)
Winfree, A.T.: Corkscrews and singularities in fruitflies: Resetting behavior of the circadian eclosion rhythm. In: Biochronometry (ed. M. Menaker), pp. 81–109. Washington: National Academy of Sciences 1971
Winfree, A.T.: On phase resetting in multicellular clockshops. In: The molecular basis of circadian rhythms (eds. J.W. Hastings, H.-G. Schweiger), pp. 109–129. Berlin: Abakon Verlagsgesellschaft 1976
Zimmerman, W.F.: On the absence of circadian rhythmicity inDrosophila pseudoobscura pupae. Biol. Bull.136, 494–500 (1969)
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NIH Predoctoral Trainee in Biophysics, 2 T01 GM00782-16.
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Njus, D., McMurry, L. & Hastings, J.W. Conditionality of circadian rhythmicity: Synergistic action of light and temperature. J Comp Physiol B 117, 335–344 (1977). https://doi.org/10.1007/BF00691559
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DOI: https://doi.org/10.1007/BF00691559