Summary
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1.
Concentrations of AMP, ADP and ATP are determined in total tissue and in posterior adductor muscle, hepatopancreas, mantle and residue ofMytilus edulis, in order to calculate the energy charge. This parameter was the highest in posterior adductor muscle (0.91) and the lowest in the hepatopancreas (0.69).
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2.
During exposure to air minimum levels of about 60% of the normal ATP content were determined in total mussel and the various tissues. The decrease in ATP could be accounted for by the increase in concentrations of AMP plus ADP and therefore the total adenylate pool was hardly affected.
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3.
The energy charge decreased in total animal during the initial days of anoxia, but raised again at the 7th day to a value equal to that in dead animals. The shift after 3 days of anoxia was highest in the posterior adductor muscle (0.24) and lowest in the hepatopancreas (0.14).
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4.
The energy charge was nearly the same in both “fed” and “starved” mussels and changed within the same range (0.85–0.6) during anaerobiosis. In “starved” animals, however, the minimum value was reached at an earlier stage.
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5.
Within this range key enzymes are highly sensitive to regulation by the energy charge according to the theory of Atkinson (1968).
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Abbreviations
- AMP, ADP, ATP :
-
adenosine-5′-mono-, di-, triphosphate
- NADH :
-
β-nicotinamide-adenine dinucleotide (reduced)
- TCA :
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trichloroacetic acid
- PCA :
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perchloric acid
- S.D. :
-
standard deviation
- PEP :
-
phosphoenolpyruvate
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Wijsman, T.C.M. Adenosine phosphates and energy charge in different tissues ofMytilus edulis L. under aerobic and anaerobic conditions. J Comp Physiol B 107, 129–140 (1976). https://doi.org/10.1007/BF00691220
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DOI: https://doi.org/10.1007/BF00691220