Abstract
Autotrophic cultures of the facultative chemolithotrophAlcaligenes eutrophus have been found to excrete glycollate. This excretion was greatly stimulated by the incorporation of up to 20% (v/v) oxygen in the hydrogen used for gassing. The stimulatory effect of oxygen was prevented by the addition of 10% (v/v) CO2 to the gassing mixture. Glycollate excretion only in the presence of oxygen was increased by the addition of 2-pyridyl-hydroxymethane sulphonic acid (HPMS), an inhibitor of glycollate oxidation, indicating that glycollate formation itself was stimulated by oxygen. No glycollate excretion by cultures grown heterotrophically on pyruvate was detected, either in the absence or presence of HPMS, under heterotrophic or autotrophic conditions.
Extracts from autotrophic cells showed phosphoglycollate phosphatase and glycollate oxidoreductase activities, which were considerably lower in extracts prepared from pyruvate- or fructose-grown (heterotrophic) cells. The increase in activity of both enzymes upon cell transfer from heterotrophic to autotrophic growth was prevented by chloramphenicol and resembled the induction ofd0ribulose 1,5-diphosphate carboxylase under the same conditions.
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Abbreviations
- DTE:
-
dithioerythritol
- EDTA:
-
ethylenediamine tetraacetate
- HPMS:
-
2-pyridyl-hydroxymethane sulphonie acid
- RuDP:
-
d-ribulose 1,5-diphosphate
References
Anderson, L., Fuller, R. C.: Photosynthesis inRhodospirillum rubrum. II. Photoheterotrophic carbon dioxide fixation. Plant Physiol.42, 491–496 (1967)
Andrews, T. J., Lorimer, G. H., Tolbert, N.E.: Incorporation of molecular oxygen into glycine and serine during photorespiration in spinach leaves. Biochemistry10, 4777–4782 (1971)
Asami, S., Akazawa, T.: Oxidative formation of glycolic acid in photosynthesizing cells ofChromatium. Plant Cell Physiol.15, 571–576 (1974)
Bassham, J. A., Kirk, M.: Sequence of formation of phospho-glycolate and glycolate in photosynthesizingChlorella pyrenoidosa. Plant Physiol.52, 407–411 (1973)
Bowes, G., Ogren, W. L., Hageman, R. H.: Phosphoglycolate production catalyzed by ribulose diphosphate carboxylase. Biochem. biophys. Res. Commun.45, 716–722 (1971)
Bowien, B., Mayer, F., Codd, G. A., Schlegel, H. G.: Purification, some properties, and quaternary structure of thed-ribulose 1,5-diphosphate carboxylase ofAlcaligenes eutrophus. Arch. Microbiol.110, 157–166 (1976)
Calkins, V. P.: Microdetermination of glycolic and oxalic acids. Ind. Engng. Chem. Anal. Ed.15, 762–763 (1943)
Cheng, K. H., Miller, A. G., Colman, B.: An investigation of glycolate excretion in two species of blue-green algae. Planta (Berl.)103, 110–116 (1972)
Codd, G. A.: Carbon dioxide metabolism in synchronously dividing cultures ofEuglena gracilis. Ph. D. Thesis, University of Bradford (1970)
Codd, G. A., Bowien, B., Schlegel, H. G.: Glycollate formation and ribulose 1,5-diphosphate carboxylase/oxygenase in a facultative chemolitho-autotrophic bacterium. Proc. Soc. gen. Microbiol.3, 8 (1975)
Codd, G. A., Schmid, G. H.: Serological characterization of the glycolate-oxidising enzymes from tobacco,Euglena gracilis and a yellow mutant ofChlorella vulgaris Plant Physiol.50, 769–773 (1972)
Codd, G. A., Smith, B. M.: Glycollate formation and excretion by the purple photosynthetic bacteriumRhodospirillum rubrum. FEBS Letters48, 105–108 (1974)
Codd, G. A., Turnbull, F.: Enzymes of glycollate formation and oxidation in two members of the Rhodospirillaceae (purple non-sulphur bacteria) Arch. Microbiol.104, 155–158 (1975)
Cohen Y., Kuenen, J. G.: Growth yields and excretion products ofThiobacillus neapolitanus grown in the chemostat. Abstr. Ann. Meeting ASM, I 56, p. 120 (1976)
Döhler, G., Braun, F.: Untersuchung der Beziehung zwischen extracellulärer Glykolsäure-Ausscheidung und der photosynthetischen CO2-Aufnahme bei der BlaualgeAnacystis nidulans. Planta (Berl.)98, 357–361 (1971)
Eberhardt, U.: Über das Wasserstoff aktivierende System vonHydrogenomonas H 16. II. Abnahme der Aktivität bei heterotrophem Wachstum. Arch. Mikrobiol.54, 115–124 (1966)
Eichenbusch, J. D., Beck, E.: Evidence for involvement of two types of reaction in glycolate formation during photosynthesis in isolated spinach chloroplasts. FEBS Letters31, 225–228 (1973)
Gottschalk, G., Eberhardt, U., Schlegel, H. G.: Verwertung von Fructose durchHydrogenomonas H 16 (I). Arch. Mikrobiol.48, 95–108 (1964)
Han, T.-W., Eley, J. H.: Glycolate excretion byAnacystis nidulans: Effect of bicarbonate concentration, oxygen concentration and light intensity. Plant Cell Physiol.14, 285–291 (1973)
Hirsch, P., Georgiev, G., Schlegel, H. G.: CO2-Fixierung durch Knallgasbakterien. III. Autotrophe und organotrophe CO2-Fixierung. Arch. Mikrobiol.46, 79–95 (1963)
McFadden, B. A., Tabita, F. R.:d-Ribulose-1,5-diphosphate carboxylase and the evolution of autotrophy. Biosystems6, 93–112 (1974)
Lowry, O. H., Rosebrough, N. J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951)
Schlegel, H. G., Kaltwasser, H., Gottschalk, G.: Ein Submersverfahren zur Kultur wasserstoffoxydierender Bakterien: Wachstumsphysiologische Untersuchungen. Arch. Mikrobiol.38, 209–222 (1961)
Tolbert, N. E.: Photorespiration. In: Algal physiology and biochemistry (W. D. P. Stewart, ed.), pp. 474–504. Oxford: Blackwell 1974
Zelitch, I.: Photosynthesis, photorespiration and plant productivity, pp. 89–122. London-New York: Academic Press 1971
Zelitch, I.: Plant productivity and the control of photorespiration. Proc. nat. Acad. Sci. (Wash.)70, 579–584 (1973)
Zelitch, I.: Pathways of carbon fixation in green plants. Ann. Rev. Biochem.44, 123–145 (1975)
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Codd, G.A., Bowien, B. & Schlegel, H.G. Glycollate production and excretion byAlcaligenes eutrophus . Arch. Microbiol. 110, 167–171 (1976). https://doi.org/10.1007/BF00690224
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DOI: https://doi.org/10.1007/BF00690224