Summary
Simultaneous measurements of transepithelial potential difference (PD) and net water flux were made in the stripped seawater eel intestine, and the effects of removal of Ca2+ and replacement of Cl− with other anions on these two parameters were examined. Removal of Ca2+ from normal (NaCl) Ringer solution on both mucosal and serosal sides reduced the serosa-negative PD and the net water flux. Since SO 2−4 binds Ca2+ strongly, the effects of substitution of SO 2−4 for Cl− could be due to deficiency in both Cl− and Ca2+. Among five anions used in this study, CH3SO −4 (with low affinity to Ca2+) seems to be the most suitable substitute for Cl−. When both mucosal and serosal Cl− were replaced with CH3SO −4 , both the PD and the net water flux decreased to approximately zero. When mucosal Cl− was replaced progressively with other anions, the serosa-negative PD and the net water flux decreased in association with the decrease in Cl− concentration, and a linear regression was observed between the decrease in the net water flux and that in the PD. These results indicate that Na+ and water transport depend closely on Cl− transport.
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References
Ando, M.: Intestinal water transport and chloride pump in relation to sea-water adaptation of the eel,Anguilla japonica. Comp. Biochem. Physiol.52A, 229–233 (1975)
Ando, M., Kobayashi, M.: Effects of stripping of the outer layers of the eel intestine on salt and water transport. Comp. Biochem. Physiol.61A, 497–501 (1978)
Ando, M., Utida, S., Nagahama, H.: Active transport of chloride in eel intestine with special reference to sea water adaptation. Comp. Biochem. Physiol.51A, 27–32 (1975)
Bolufer, J., Larralde, J.: Different response of the bird and mammal intestinal active transport to calcium. Comp. Biochem. Physiol.58A, 75–79 (1977)
Christoffersen, G.R.J., Skibsted, L.H.: Calcium ion activity in physiological salt solutions: Influence of anions substituted for chloride. Comp. Biochem. Physiol.52A, 317–322 (1975)
Field, M., Karnaky Jr., K.J., Smith, P.L., Bolton J.E.: Ion transport across the isolated intestinal mucosa of the winter flounder,Pseudopleuronectes americanus. J. Membr. Biol.41, 265–293 (1978)
Frizzell, R.A., Schultz, S.G.: Ionic conductance of extracellular shunt pathway in rabbit ileum. Influence of shunt on transmural sodium transport and electrical potential differences. J. Gen. Physiol.59, 318–346 (1972)
Frizzell, R.A., Nellans, H.N., Rose, R.C., Markscheid-Kaspi, L., Schultz, S.G.: Intracellular Cl concentrations and influxes across the brush border of rabbit ileum. Am. J. Physiol.224, 328–337 (1973)
House, C.R.: Water transport in cells and tissues. London: Edward Arnold 1974
Huang, K.C., Chen, T.S.T.: Ion transport across intestinal mucosa of winter flounder,Pseudopleuronectes americanus. Am J. Physiol.220, 1734–1738 (1971)
LeFevre, M.E., Reisman, L.: Structure, function, and long-term maintenance of the isolated turtle colon. J. Exp. Zool.203, 443–454 (1978)
Skadhauge, E.: The mechanism of salt and water absorption in the intestine of the eel (Anguilla anguilla) adapted to waters of various salinities. J. Physiol.204, 135–158 (1969)
Skadhauge, E.: Coupling of transmural flows of NaCl and water in the intestine of the eel. J. Exp. Biol.60, 535–546 (1974)
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Ando, M. Chloride-dependent sodium and water transport in the seawater eel intestine. J Comp Physiol B 138, 87–91 (1980). https://doi.org/10.1007/BF00688739
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DOI: https://doi.org/10.1007/BF00688739