Summary
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1.
The activity of choline acetyltransferase (ChAT) has been determined in different parts of the swimbladder of two physostome species, the rainbow trout,Salmo gairdneri, and the eel,Anguilla anguilla, and three physoclist species, the Atlantic cod,Gadus morhua, the perch,Perca fluviatilis and the goldsinny wrasse,Ctenolabrus rupestris.
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2.
High activities of ChAT were found in the gas gland of the physoclist species. Preganglionic denervation (in the cod) only partly reduces this activity. It is concluded that the gas gland has a postganglionic cholinergic innervation.
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3.
The secretory mucosa outside the gas gland shows intermediate ChAT activity in the wrasse and low activity in the cod and the perch.
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4.
Very low activities of ChAT were found in the oval edge of the cod and the perch, indicating little if any cholinergic innervation of this area. The resorbent area of the wrasse swimbladder shows an intermediate activity.
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5.
In the eel the secretory mucosa shows low ChAT activity, indicating sparse innervation of the secretory cells. The resorbent mucosa contains a much higher activity.
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6.
The rainbow trout has an evenly distributed intermediate ChAT activity in the swimbladder.
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Abbreviations
- ChAT :
-
choline acetyltransferase
References
Abrahamsson T, Nilsson S (1975) Effects of nerve sectioning and drugs on the catecholamine content in the spleen of the cod,Gadus morhua. Comp Biochem Physiol [C] 51:231–233
Augustinsson K-B, Fänge R (1951) Innervation and acetylcholine splitting activity of the airbladder of fishes. Acta Physiol Scand 22:224–230
Barajas L, Wang P (1975) Demonstration of acetylcholinesterase in adrenergic nerves of renal glomerular arterioles. J Ultrastruct Res 53:244–253
Bohr C (1894) The influence of section of the vagus nerve on disengagement of gases in the air-bladder of fishes. J Physiol 15:494–500
Burnstock G (1979) The ultrastructure of autonomic cholinergic nerves and junctions. Prog Brain Res 49:3–21
Deineka D (1905) Zur Frage über den Bau der Schwimmblase. Z Wiss Zool 78:149–164
Dreser H (1892) Notiz über eine Wirkung des Pilocarpins. Arch Exp Pathol Pharmakol 30:159–160
Ehinger B, Falck B, Persson H, Rosengren A-M, Rosengren E (1966) Choline acetylase activity in the normal and denervated cat iris. Life Sci 5:481–483
Ekström J, Elmér M (1977) Choline acetyltransferase activity in the denervated urinary bladder of the rat. Acta Physiol Scand 101:58–62
Eränkö O, Rechardt L, Eränkö L, Cunningham A (1970) Light and electron microscopic histochemical observations on cholinesterase-containing sympathetic nerve fibres in the pineal body of the rat. Histochem J 2:479–489
Fonnum (1969) Radiochemical micro assay for determination of choline acetyltransferase and acetylcholinesterase activities. Biochem J 115:465–472
Fänge R (1953) The mechanism of gas transport in the euphysoclist swimbladder. Acta Physiol Scand 30:1–133
Fänge R, Holmgren S, Nilsson S (1976) Autonomic nerve control of the swimbladder of the goldsinny wrasse,Ctenolabrus rupestris. Acta Physiol Scand 97:292–303
Hebb CO, Ratkovic D (1962) Choline acetylase in the placenta of man and other species. J Physiol (Lond) 163:307–313
Jacobs W (1930) Untersuchungen zur Physiologie der Schwimmblase. I. Über Gassekretion in der Schwimmblase von Physoclisten. Z Vergl Physiol 11:565–629
Lund DD, Schmid PG, Roskoski R (1979) Choline acetyltransferase activity in rat guinea pig heart following vagotomy. Am J Physiol 236:H620-H623
McLean JR, Nilsson S (1981) A histochemical study of the gas gland innervation in the Atlantic cod,Gadus morhua. Acta Zool 62:187–194
Nilsson S (1971) Adrenergic innervation and drug responses of the oval sphincter in the swimbladder of the cod (Gadus morhua). Acta Physiol Scand 83:446–453
Nilsson S (1976) Fluorescent histochemistry and cholinesterase staining of sympathetic ganglia in a teleost,Gadus morhua. Acta Zool (Stockh) 57:69–77
Roskoski R, McDonald RI, Roskoski LM, Marwin MJ, Hermsmeyer K (1977) Choline acetyltransferase activity in heart: evidence for neuronal and not myocardial origin. Am J Physiol 233:H642-H646
Ross LG (1978) The innervation of the resorptive structures in the swimbladder of a physoclist fishPollachius virens (L.). Comp Biochem Physiol [C] 61:385–388
Saupe M (1940) Anatomie und Histologie der Schwimmblase des Flußbarsches (Perca fluviatilis) mit besonderer Berücksichtigung des Ovals. Z Zellforsch Mikrosk Anat 30:1–35
Steen JB (1963) The physiology of the swimbladder of the eel,Anguilla vulgaris. III. The mechanism of gas secretion. Acta Physiol Scand 59:221–241
Stray-Pedersen S (1970) Vascular responses induced by drugs and by vagal stimulation in the swimbladder of the eel,Anguilla vulgaris. Comp Gen Pharmacol 1:358–364
Tuček S (1974) Transport and changes in activity of choline acetyltransferase in the peripheral stump of an interrupted nerve. Brain Res 82:249–261
Welsch F (1977) The cholinergic system in tissues without innervation. Choline acetyltransferase, choline and acetylcholine in the placenta of the rhesus monkey (Macaca mulatta). Biochem Pharmacol 26:1281–1286
Winberg M, Holmgren S, Nilsson S (1981) Effects of denervation and 6-hydroxydopamine on the activity of choline acetyltransferase in the spleen of the cod,Gadus morhua. Comp Biochem Physiol [C] 69:141–143
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Fänge, R., Holmgren, S. Choline acetyltransferase activity in the fish swimbladder. J Comp Physiol B 146, 57–61 (1982). https://doi.org/10.1007/BF00688717
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DOI: https://doi.org/10.1007/BF00688717