Summary
A single intravenous injection of 30 mg of N-ethyl-N-nitrosourea per kg of body weight was administered to pregnant BD-IX rats on the 15th day of gestation. The neural tumours which developed in 97.5% of the offspring were gliomas and schwannomas. The demonstration of acid phosphatase in the neoplastic and reacting cells of these tumours was carried out at fine structural level. The enzyme activity was localised in lysosomes and in the innermost cisternae of the Golgi complexes. High acid phosphatase activity was related not only to degeneration, necrosis and phagocytosis but also to cellular differentiation and anaplasia.
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References
Barron, K. D.: Enzyme histochemistry of the central nervous system. In: J. Minckler (ed.): Pathology of the nervous system, pp. 177–227. New York: McGraw-Hill 1968
Druckrey, H., Ivankovic, S., Preussmann, R.: Selektive Erzeugung von Hirntumoren bei Ratten durch methylnitrosoharnstoff. Naturwissenschaften51, 144 (1964)
Druckrey, H., Ivankovic, S., Preussmann, R.: Selektive Erzeugung maligner Tumoren in Gehirn und Rückenmark von Ratten durch N-Methyl-N-nitrosoharnstoff. Z. Krebsforsch.66, 389–408 (1965)
Druckrey, H., Ivankovic, S., Preussmann, R.: Teratogenic and carcinogenic effects in the offspring after single injection of ethylnitrosourea to pregnant rats. Nature (Lond.)210, 1378–1379 (1966)
Druckrey, H., Ivankovic, S., Preussmann, R., Zülch, K. J., Mennel, H. D.: Selective induction of malignant tumors of the nervous system by resorptive carcinogens. In: W. M. Kirsch, E. Grossi-Paoletti, and P. Paoletti (eds.): The experimental biology of brain tumors, pp. 85–147. Springfield, Ill.: Ch. C. Thomas 1972
Druckrey, H., Landschütz, C., Ivankovic, S.: Transplacentare Erzeugung maligner Tumoren des Nervensystems. II. Äthyl-nitrosoharnstoff an 10 genetisch definierten Rattenstämmen. Z. Krebsforsch.73, 371–386 (1970)
Fabiani, A., Schiffer, D., Paoletti, P., Grossi-Paoletti, E.: Histochemical evaluation of hydrolytic enzymes in tumors induced in the rat by nitrosourea derivatives. Acta neuropath. (Berl.)15, 272–278 (1970)
Goldfischer, S., Essner, E., Novikoff, A. B.: The localization of phosphatase activities at the level of ultrastructure. J. Histochem. Cytochem.12, 72–95 (1964)
Gomori, G.: Microtechnical demonstration of phosphatase in tissue sections. Proc. Soc. exp. Biol. (N. Y.)42, 23–26 (1939)
Gomori, G.: Microscopic histochemistry. Principles and practice. Chicago: The University of Chicago Press 1952
Holt, S. J.: Personal communication (1969)
Ivankovic, S., Druckrey, H.: Transplacentare Erzeugung maligner Tumoren des Nervensystems. I. Äthyl-nitrosoharnstoff (ÄNH) an BD IX-Ratten. Z. Krebsforsch.71, 320–360 (1968)
Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol.27 137 A (1965)
Lantos, P. L.: The fine structure and enzyme cytochemistry of tumours induced by N-ethyl-N-nitrosourea in the nervous system of rats. Ph. D. Thesis, University of London 1973
Leduc, E. H., Bernhard, W., Holt, S. J., Tranzer, J. P.: Ultrathin frozen sections. II. Demonstration of enzymic activity. J. Cell Biol.34, 773–785 (1967)
Lolova, I., Ivanova, A., Bojinov, S.: Histochemical investigation of nonspecific acid phosphatase in cerebral tumors. J. Neurosurg.34, 730–740 (1971)
Meyer, P.: Histochemistry of the developing human brain. Acta neurol. scand.39, 123–138 (1963)
Millonig, G.: Advantages of a phosphate buffer for osmium tetroxide solutions in fixation. J. appl. Physics.32, 1637 (1961)
Novikoff, A. B.: Lysosomes in the physiology and pathology of cells: Contributions of staining methods. In: A. V. S. de Reuck and M. P. Cameron (eds.): Ciba Symp. on Lysosomes, pp. 36–77. London: J. and A. Churchill Ltd. 1963
Novikoff, A. B.: Enzyme localization and ultrastructure of neuron. Lysosomes in nerve cells. In: H. Hydén (ed.): The neuron, pp. 255–318. Amsterdam: Elsevier 1967
Novikoff, A. B., Essner, E.: Pathological changes in cytoplasmic organelles. Fed. Proc.21, 1130–1142 (1962)
Novikoff, A. B., Holtzman, E.: Lysosomes. In: Cells and organelles, pp. 121–135. New York: Holt, Rinehart and Winston, Inc. 1970
Osske, G., Schreiber, D., Schneider, J., Jänisch, W.: Enzymhistochemische Untersuchungen an experimentellen Glioblastomen des Kaninchens. Europ. J. Cancer5, 525–531 (1969a)
Osske, G., Warzok, R.: Enzymhistochemische Befunde an einem wiederholt transplantierten Nerventumor der Ratte. Exp. Path.6, 145–150 (1972)
Osske, G., Warzok, R., Jänisch, W.: Enzymhistochemische Untersuchungen an experimentellen Hirntumoren der Ratte. Exp. Path.3, 280–288 (1969b)
Schiffer, D.: Histochemistry of hydrolytic enzymes in cerebral tumors. The relationship between the different cytotypes and cell regression. J. Neurosurg.34, 456–457 (1971)
Schiffer, D., Fabiani, A., Monticone, G. F., Gabella, G.: Histochemical study of acid phosphatase activity in cerebral tumors. Acta neuropath. (Berl.)5, 16–25 (1965)
Stavrou, D.: Zur Morphologie und Histochemie experimentell induzierter Hirntumoren beim Kaninchen. Z. Krebsforsch.73, 98–109 (1969)
Stavrou, D.: Beitrag zur Morphologie und Enzymhistochemie experimenteller Tumoren des Zentralnervensystems der Ratte. II. Enzymhistochemische Befunde. Acta neuropath. (Berl.)15, 231–239 (1970)
Swenberg, J. A., Koestner, A.: Histochemical studies on selected enzymes of experimental neuroectodermal tumors. Progr. exp. Tumor Res. (Basel)17, 328–345 (1972)
Wechsler, W.: Old and new concepts of oncogenesis in the nervous system of man and animals. Progr. exp. Tumor Res. (Basel)17, 219–278 (1972)
Wechsler, W., Kleihues, P., Matsumoto, S., Zülch, K. J., Ivankovic, S., Preussmann, R., Druckrey, H.: Pathology of experimental neurogenic tumors chemically induced during prenatal and postnatal life. Ann. N. Y. Acad. Sci.159, 360–408 (1969)
Wender, M., Kozik, M., Wojciechowski, T.: Enzymhistochemische Untersuchungen zur Entwicklung der Neuroglia des menschlichen Gehirns. Acta histochem. (jena)36, 32–43 (1970)
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Lantos, P.L. Fine structural localisation of acid phosphatase in neural tumours induced by N-ethyl-N-nitrosourea in rats. Acta Neuropathol 29, 45–55 (1974). https://doi.org/10.1007/BF00684390
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DOI: https://doi.org/10.1007/BF00684390